Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MP622_RS11030 Genome accession   NZ_CP094643
Coordinates   2341379..2342497 (+) Length   372 a.a.
NCBI ID   WP_044739263.1    Uniprot ID   A0A5N4WAN1
Organism   Acinetobacter sp. PK01     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2336379..2347497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MP622_RS11010 (MP622_11010) - 2336611..2337636 (+) 1026 WP_016165371.1 class I SAM-dependent methyltransferase -
  MP622_RS11015 (MP622_11015) - 2337646..2339109 (+) 1464 WP_243790978.1 amino acid permease -
  MP622_RS11020 (MP622_11020) - 2339502..2340188 (-) 687 WP_016165373.1 YggS family pyridoxal phosphate-dependent enzyme -
  MP622_RS11025 (MP622_11025) pilT 2340312..2341349 (+) 1038 WP_016165374.1 type IV pilus twitching motility protein PilT Machinery gene
  MP622_RS11030 (MP622_11030) pilU 2341379..2342497 (+) 1119 WP_044739263.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MP622_RS11035 (MP622_11035) omp38 2342640..2343656 (-) 1017 WP_044739262.1 outer membrane protein Omp38 -
  MP622_RS11040 (MP622_11040) - 2344034..2344552 (+) 519 WP_016165377.1 GspH/FimT family pseudopilin -
  MP622_RS11045 (MP622_11045) - 2344654..2345829 (+) 1176 WP_243790980.1 thiolase family protein -
  MP622_RS11050 (MP622_11050) - 2345972..2346418 (+) 447 WP_016165379.1 VOC family protein -
  MP622_RS11055 (MP622_11055) - 2346521..2347138 (+) 618 WP_016165380.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41843.18 Da        Isoelectric Point: 6.8365

>NTDB_id=670317 MP622_RS11030 WP_044739263.1 2341379..2342497(+) (pilU) [Acinetobacter sp. PK01]
MDFNDLLNLMIQQKASDLFVTAEVEPSMKINGQIVPVAKTKLTGEIVGQLLNSIMSDKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDQPGMVLRRIETKIPTMDELKLPPVLKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHGQLFMDLSLNLKAMVAQQLIPTIDGNSRRAAIEILINSPLISDLIRKGDIHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGADRLLNASSHITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=670317 MP622_RS11030 WP_044739263.1 2341379..2342497(+) (pilU) [Acinetobacter sp. PK01]
ATGGACTTTAACGACTTACTCAATCTCATGATTCAACAAAAAGCATCTGACCTTTTTGTCACAGCTGAAGTTGAACCATC
TATGAAAATTAATGGTCAAATTGTTCCTGTTGCGAAAACCAAGTTAACAGGTGAAATTGTTGGTCAACTCTTAAATTCAA
TTATGAGTGATAAACAACGTAAAGAATTTGCAGATACACGTGAATGTAACTTTGCGATTATGAATCGTGAAAAAACAGCA
CGTTTCCGTGTCAGTGCATTTCAACAACGTGACCAACCTGGCATGGTTCTACGTCGCATTGAAACTAAAATTCCAACCAT
GGATGAACTCAAGCTTCCTCCAGTGTTGAAAGACTTAGCCATGACCAAACGTGGCATCATTATTTTTGTGGGTGCAACAG
GTACTGGTAAATCTACTTCCTTGGCCTCAATTATTGGATATCGTAATCAAAATTCTAAAGGTCATATCATTACCATTGAA
GATCCGATCGAATTTATTCACGAACATGCAGGCTGTATTATCACTCAACGTGAAGTAGGCATCGATACCGACTCCTTTGA
AATTGCGTTGAAAAATACCTTACGTCAAGCACCCGATGTTATTCTGATTGGTGAGATTCGTTCACGCGAGACCATGGATT
ACGCAATTGCTTTCGCAGAAACAGGCCATTTGGTTTTTGCAACGCTACATGCTAACAATGCCAACCAAGCGATTGATCGT
ATCATTCACTTCTTTGAAGCAGACCGTCATGGACAATTATTCATGGACTTGTCCTTAAATTTGAAAGCCATGGTCGCTCA
GCAACTTATTCCAACGATTGATGGTAATTCACGCCGTGCTGCAATTGAGATTTTGATTAATTCACCATTAATTTCCGACT
TAATCCGGAAAGGCGATATTCACGAAATTAAAGATTTAATGAAGCGTTCGCGTGAACTCGGTATGCAAACCTTTGACCAA
GCACTTTACGATTTATATAAAGCCAAGCAGATCACTTATAAAGATGCGCTGAAACATGCCGATTCACCAAATGATTTACG
CCTACAAATCAAACTCTCTGAGGAAGGTGCTGATCGTTTATTGAATGCAAGCTCGCATATTACTTTTGATGGTCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5N4WAN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.19

100

0.774
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.731

0.677
  pilU Vibrio cholerae strain A1552

55.84

94.355

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Pseudomonas aeruginosa PAK

40.173

93.011

0.374
  pilT Acinetobacter baumannii strain A118

39.65

92.204

0.366
  pilT Acinetobacter baumannii D1279779

39.65

92.204

0.366
  pilT Acinetobacter nosocomialis M2

39.65

92.204

0.366
  pilT Legionella pneumophila strain ERS1305867

40.476

90.323

0.366
  pilT Legionella pneumophila strain Lp02

40.476

90.323

0.366
  pilT Acinetobacter baylyi ADP1

39.359

92.204

0.363
  pilT Neisseria meningitidis 8013

39.706

91.398

0.363
  pilT Neisseria gonorrhoeae MS11

39.706

91.398

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

38.177

94.355

0.36
  pilT Vibrio cholerae strain A1552

38.177

94.355

0.36
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36