Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MP622_RS11025 Genome accession   NZ_CP094643
Coordinates   2340312..2341349 (+) Length   345 a.a.
NCBI ID   WP_016165374.1    Uniprot ID   R9B9Y2
Organism   Acinetobacter sp. PK01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2335312..2346349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MP622_RS11010 (MP622_11010) - 2336611..2337636 (+) 1026 WP_016165371.1 class I SAM-dependent methyltransferase -
  MP622_RS11015 (MP622_11015) - 2337646..2339109 (+) 1464 WP_243790978.1 amino acid permease -
  MP622_RS11020 (MP622_11020) - 2339502..2340188 (-) 687 WP_016165373.1 YggS family pyridoxal phosphate-dependent enzyme -
  MP622_RS11025 (MP622_11025) pilT 2340312..2341349 (+) 1038 WP_016165374.1 type IV pilus twitching motility protein PilT Machinery gene
  MP622_RS11030 (MP622_11030) pilU 2341379..2342497 (+) 1119 WP_044739263.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MP622_RS11035 (MP622_11035) omp38 2342640..2343656 (-) 1017 WP_044739262.1 outer membrane protein Omp38 -
  MP622_RS11040 (MP622_11040) - 2344034..2344552 (+) 519 WP_016165377.1 GspH/FimT family pseudopilin -
  MP622_RS11045 (MP622_11045) - 2344654..2345829 (+) 1176 WP_243790980.1 thiolase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38313.94 Da        Isoelectric Point: 6.6653

>NTDB_id=670316 MP622_RS11025 WP_016165374.1 2340312..2341349(+) (pilT) [Acinetobacter sp. PK01]
MDITELLAFSAKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEEQLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGLGNIFKEICDYPRGIVLVTGPTGSGKSTTLAAMLDYINDRRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMSTLDQSL
KTLVSKGIISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=670316 MP622_RS11025 WP_016165374.1 2340312..2341349(+) (pilT) [Acinetobacter sp. PK01]
ATGGACATTACAGAATTATTGGCTTTTTCTGCAAAAAACGGGGCGTCTGACTTACACTTATCTGCGGGTATGCCACCAAT
GATTCGCGTTGATGGCGAAGTACGTCGTATTAACTTACCAGCTTTAGAACATAAAGAAGTGCACAAGTTAGTGTACGACA
TTATGAATGATAAGCAACGTCGTGATTATGAAGAACAATTGGAAACTGACTTCTCTTTTGAAGTACCTGGCGTTGCCCGT
TTCCGTGTAAACGCATTTAATCAAAACCGCGGTGCTGGTGCCGTATTCCGTACCATTCCATCCAAAGTTCTCACCATGGA
TGACCTTGGTCTAGGCAATATTTTTAAAGAAATTTGTGACTACCCACGTGGCATCGTATTAGTTACAGGACCAACTGGTT
CTGGTAAATCCACCACCTTAGCCGCGATGCTAGATTATATTAACGATCGACGTTATGACCATATTTTAACGGTCGAAGAT
CCAATCGAATTTGTACACCAATCGAAAAAATGTCTAATTAACCAACGTGAAGTACATCGTGACACGCATGGTTTTAATGA
AGCATTACGTTCTGCACTGCGTGAAGACCCTGACATTATTTTGGTTGGTGAGATGCGTGACCTTGAAACTATTCGCCTTG
CCTTAACTGCGGCGGAAACGGGTCACTTGGTTTTCGGTACACTACATACGACCTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGATATGGTTCGTGCCATGCTGTCTGAATCTTTACAGGCCGTTATTTCACAAAC
ACTCTTAAAGAAAAATGGCGGTGGTCGTGTCGCTGCGCATGAAATCATGATTGGTATCCCAGCAATTCGTAACTTAATTC
GTGAGAACAAAGTTGCACAAATGTACTCTGCCATCCAAACTGGTGCTAACTATGGTATGTCTACCTTAGACCAAAGCCTT
AAAACGCTCGTATCTAAAGGCATCATTAGTCCACAAACAGCACGTACTGTTGCTAAACAACCAGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R9B9Y2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.362

100

0.954
  pilT Acinetobacter baumannii D1279779

95.072

100

0.951
  pilT Acinetobacter baumannii strain A118

95.072

100

0.951
  pilT Acinetobacter baylyi ADP1

87.791

99.71

0.875
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.75

97.391

0.67
  pilT Vibrio cholerae strain A1552

68.75

97.391

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.595

100

0.397
  pilU Pseudomonas stutzeri DSM 10701

40

97.101

0.388