Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MRB47_RS02675 Genome accession   NZ_CP094346
Coordinates   557806..558849 (-) Length   347 a.a.
NCBI ID   WP_011803881.1    Uniprot ID   A0AAX1WY06
Organism   Diaphorobacter sp. LI3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 552806..563849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRB47_RS02645 (MRB47_02645) - 553107..553505 (+) 399 WP_252101825.1 YraN family protein -
  MRB47_RS02650 (MRB47_02650) - 553564..554157 (+) 594 WP_011803876.1 SIS domain-containing protein -
  MRB47_RS02655 (MRB47_02655) - 554154..554828 (+) 675 WP_252101826.1 BON domain-containing protein -
  MRB47_RS02660 (MRB47_02660) - 554926..555852 (-) 927 WP_252101827.1 NAD(P)-dependent oxidoreductase -
  MRB47_RS02665 (MRB47_02665) pilU 555917..557053 (-) 1137 WP_252101828.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MRB47_RS02670 (MRB47_02670) - 557100..557729 (-) 630 WP_047350275.1 Crp/Fnr family transcriptional regulator -
  MRB47_RS02675 (MRB47_02675) pilT 557806..558849 (-) 1044 WP_011803881.1 type IV pilus twitching motility protein PilT Machinery gene
  MRB47_RS02680 (MRB47_02680) - 558889..559605 (+) 717 WP_252101829.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRB47_RS02685 (MRB47_02685) - 559644..560639 (+) 996 Protein_534 aminotransferase class V-fold PLP-dependent enzyme -
  MRB47_RS02690 (MRB47_02690) - 560635..561207 (-) 573 Protein_535 type IV pili methyl-accepting chemotaxis transducer N-terminal domain-containing protein -
  MRB47_RS02695 (MRB47_02695) - 561240..562625 (-) 1386 WP_252101831.1 FAD-binding oxidoreductase -
  MRB47_RS02700 (MRB47_02700) - 562626..563333 (-) 708 WP_235103467.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38120.87 Da        Isoelectric Point: 6.7742

>NTDB_id=669144 MRB47_RS02675 WP_011803881.1 557806..558849(-) (pilT) [Diaphorobacter sp. LI3]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKTYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNESEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LSDLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=669144 MRB47_RS02675 WP_011803881.1 557806..558849(-) (pilT) [Diaphorobacter sp. LI3]
GTGGACATCACCCAATTGCTCGCCTTCAGCGTGAAGAACAAGGCGTCCGACCTGCACCTTTCCGCCGGCCTGCCGCCCAT
GATCCGGGTCCACGGGGACGTACGCCGCATCAACGTCGATGCACTGGACCACAAGACCGTCCACGCCATGGTGTACGACA
TCATGAGCGACGCCCAGCGCAAGACCTACGAAGAGTTCCTGGAAGTGGACTTTTCCTTCGAGATCGACGGCCTGGCGCGC
TTTCGCGTCAACGCGTTCAACCAGAACCGCGGCGCGGCCGCGGTGTTCCGTACCATTCCGAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCCAAGATCTTTGCGGACCTGGCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACGGGCCCCACAGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGGTCAACTACCTCAACGAAAGCGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAGCGTGAAGTCGGCCCGATGACGCTGTCGTTCGCGGC
CGCGCTGAAGTCCGCCCTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACCGCCGCGGAAACGGGCCACTTGGTGTTCGGCACGTTGCACACGTCCAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCGATGTTGTCGGAGTCGCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGTTCTGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACAAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCACAGATGTACTCCACCATCCAGACCAGCAACAGCGTGGGCATGCAGACGCTGGACCAGAAC
CTCTCTGACCTGGTGCGCCGCAACATCATCAGCCCGGCCGAGGCGCGCAGCAAGGCCAAGATTCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.742

98.271

0.666
  pilT Vibrio cholerae strain A1552

67.742

98.271

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.424

0.496
  pilU Vibrio cholerae strain A1552

44.776

96.542

0.432
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment