Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MRB47_RS02665 Genome accession   NZ_CP094346
Coordinates   555917..557053 (-) Length   378 a.a.
NCBI ID   WP_252101828.1    Uniprot ID   -
Organism   Diaphorobacter sp. LI3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 550917..562053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRB47_RS02635 (MRB47_02635) - 551021..551950 (+) 930 WP_252101823.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  MRB47_RS02640 (MRB47_02640) rsmI 552169..553107 (-) 939 WP_252101824.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  MRB47_RS02645 (MRB47_02645) - 553107..553505 (+) 399 WP_252101825.1 YraN family protein -
  MRB47_RS02650 (MRB47_02650) - 553564..554157 (+) 594 WP_011803876.1 SIS domain-containing protein -
  MRB47_RS02655 (MRB47_02655) - 554154..554828 (+) 675 WP_252101826.1 BON domain-containing protein -
  MRB47_RS02660 (MRB47_02660) - 554926..555852 (-) 927 WP_252101827.1 NAD(P)-dependent oxidoreductase -
  MRB47_RS02665 (MRB47_02665) pilU 555917..557053 (-) 1137 WP_252101828.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MRB47_RS02670 (MRB47_02670) - 557100..557729 (-) 630 WP_047350275.1 Crp/Fnr family transcriptional regulator -
  MRB47_RS02675 (MRB47_02675) pilT 557806..558849 (-) 1044 WP_011803881.1 type IV pilus twitching motility protein PilT Machinery gene
  MRB47_RS02680 (MRB47_02680) - 558889..559605 (+) 717 WP_252101829.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRB47_RS02685 (MRB47_02685) - 559644..560639 (+) 996 Protein_534 aminotransferase class V-fold PLP-dependent enzyme -
  MRB47_RS02690 (MRB47_02690) - 560635..561207 (-) 573 Protein_535 type IV pili methyl-accepting chemotaxis transducer N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41729.18 Da        Isoelectric Point: 6.9080

>NTDB_id=669143 MRB47_RS02665 WP_252101828.1 555917..557053(-) (pilU) [Diaphorobacter sp. LI3]
MERDQASKFINDLLKLMVSRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPNHTLTLARAIMSDKQVADFERTKECNFAI
SPAGVGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMLDWRNENSFG
HIVTVEDPVEFVHPHKNCVITQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKALISQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQALFDLFEANVISYEDALRNADSVNDLRLQIKLSSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=669143 MRB47_RS02665 WP_252101828.1 555917..557053(-) (pilU) [Diaphorobacter sp. LI3]
ATGGAACGGGATCAGGCGAGTAAATTCATCAACGATCTGCTCAAGCTGATGGTCAGCCGCGGGGGGAGCGACCTTTTCAT
CACGGCGGAGTTTCCGCCGGCGATCAAGGTCGACGGCAAGGTCACCAAGGTCTCTCCCCAGCCTCTGACACCCAACCACA
CGCTCACGCTGGCGCGCGCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACCAAGGAGTGCAACTTTGCCATC
TCGCCTGCGGGCGTGGGCCGGTTCCGCGTGAACGCCTTCATACAGCAGGGCCGCGTGGGCATGGTGCTGCGGACGATTCC
GCTCACGCTGCCCACCATCGACGGCCTGGGCGTTCCGCAGGTGCTCAAGGAAGTCACCATGACCAAGCGCGGACTGTGCA
TCCTGGTCGGCGCGACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGCTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCGTCACGGTGGAAGACCCGGTGGAATTCGTGCATCCGCACAAAAACTGCGTGATCACGCAGCGCGAGGTGGGCCT
GGACACCGACAGCTGGGAGGCGGCCCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCGTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGGCGCGCCCAGCTGCTGATGGATCTGTCGCTGAACCT
CAAGGCACTGATCTCGCAGCGCCTGATTCCCAAGCAGGACGGCAAGGGGCGCGCGGCCGCCGTCGAGGTGATGCTGAACA
CGCCGCTGATCGCCGACCTCATCTTCAAGGGCGAGGTGGCCGAGATCAAGGAAATCATGAAGAAAAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCACTGTTCGACCTGTTCGAGGCCAACGTGATCAGCTATGAGGATGCCCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTCAGCAGCCAGCGTGCCAAGACGACGGACCTGGCCTCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

58.659

94.709

0.556
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Pseudomonas aeruginosa PAK

45.994

89.153

0.41
  pilT Acinetobacter baumannii strain A118

45.946

88.095

0.405
  pilT Acinetobacter nosocomialis M2

45.946

88.095

0.405
  pilT Acinetobacter baumannii D1279779

45.946

88.095

0.405
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

44.214

89.153

0.394
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Acinetobacter baylyi ADP1

43.544

88.095

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment