Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PM1_RS28070 Genome accession   NZ_CP094343
Coordinates   6314457..6315491 (-) Length   344 a.a.
NCBI ID   WP_009615237.1    Uniprot ID   -
Organism   Pseudomonas sp. M1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6309457..6320491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PM1_RS28050 (MRY70_28050) - 6309660..6310832 (-) 1173 WP_009615241.1 cystathionine gamma-synthase -
  PM1_RS28055 (MRY70_28055) - 6310829..6312202 (-) 1374 WP_009615240.1 pyridoxal-phosphate dependent enzyme -
  PM1_RS28060 (MRY70_28060) - 6312509..6312913 (-) 405 WP_009615239.1 TM2 domain-containing protein -
  PM1_RS28065 (MRY70_28065) pilU 6313207..6314352 (-) 1146 WP_009615238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PM1_RS28070 (MRY70_28070) pilT 6314457..6315491 (-) 1035 WP_009615237.1 type IV pilus twitching motility protein PilT Machinery gene
  PM1_RS28075 (MRY70_28075) - 6315565..6316254 (+) 690 WP_024128804.1 YggS family pyridoxal phosphate-dependent enzyme -
  PM1_RS28080 (MRY70_28080) proC 6316271..6317089 (+) 819 WP_009615235.1 pyrroline-5-carboxylate reductase -
  PM1_RS28085 (MRY70_28085) - 6317100..6317693 (+) 594 WP_009615234.1 YggT family protein -
  PM1_RS28090 (MRY70_28090) - 6317693..6317989 (+) 297 WP_009615233.1 DUF167 domain-containing protein -
  PM1_RS28095 (MRY70_28095) - 6318101..6320068 (+) 1968 WP_009615232.1 dynamin-like GTPase family protein -
  PM1_RS28100 (MRY70_28100) - 6320131..6320322 (+) 192 WP_009615231.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37852.73 Da        Isoelectric Point: 6.7752

>NTDB_id=669111 PM1_RS28070 WP_009615237.1 6314457..6315491(-) (pilT) [Pseudomonas sp. M1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDVPRGLVLVTGPTGSGKSTSLAAMLDYLNNTKYSHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKAMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
KGLVAKGLVTRDAAKEKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=669111 PM1_RS28070 WP_009615237.1 6314457..6315491(-) (pilT) [Pseudomonas sp. M1]
ATGGATATTACCGAGTTGCTCGCCTTCAGCGCCAAGCAAGGCGCTTCGGACCTGCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCAACCTGCCGCCGCTGGAGCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTGGAAACCGACTTCTCCTTCGAAGTGCCGGGCGTGGCGCGT
TTCCGCGTCAATGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTGCTGACCATGGA
GGACCTGGGCATGGGCGAAGTGTTCAAGCGGGTTTCCGACGTGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCTCGCTCGCGGCGATGCTCGACTACCTGAACAACACCAAGTACAGCCACATCCTCACCGTCGAGGAC
CCGATCGAGTTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTCGGCTTCAACGA
GGCGCTGCGCTCGGCCCTGCGTGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGCCGAGGAGAAGGCCATGGTCCGTTCCATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
GCTGCTGAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCGGCGCGCTGGGCATGCAGACCCTCGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGCCTGGTGACCCGCGACGCGGCGAAGGAAAAGGCGAAGATTCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.93

100

0.959
  pilT Pseudomonas stutzeri DSM 10701

93.314

100

0.933
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

98.837

0.677
  pilT Vibrio cholerae strain A1552

68.529

98.837

0.677
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment