Detailed information NTG0669110

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PM1_RS28065
• Genome accession: NZ_CP094343
• Coordinates: 6313207..6314352 (-)
• Length: 381 a.a.
• NCBI ID: WP_009615238.1
• Uniprot ID: A0A1A5D0E6
• Organism: Pseudomonas sp. M1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6308207..6319352

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PM1_RS28050 (MRY70_28050)	-	6309660..6310832 (-)	1173	WP_009615241.1	cystathionine gamma-synthase	-
PM1_RS28055 (MRY70_28055)	-	6310829..6312202 (-)	1374	WP_009615240.1	pyridoxal-phosphate dependent enzyme	-
PM1_RS28060 (MRY70_28060)	-	6312509..6312913 (-)	405	WP_009615239.1	TM2 domain-containing protein	-
PM1_RS28065 (MRY70_28065)	pilU	6313207..6314352 (-)	1146	WP_009615238.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PM1_RS28070 (MRY70_28070)	pilT	6314457..6315491 (-)	1035	WP_009615237.1	type IV pilus twitching motility protein PilT	Machinery gene
PM1_RS28075 (MRY70_28075)	-	6315565..6316254 (+)	690	WP_024128804.1	YggS family pyridoxal phosphate-dependent enzyme	-
PM1_RS28080 (MRY70_28080)	proC	6316271..6317089 (+)	819	WP_009615235.1	pyrroline-5-carboxylate reductase	-
PM1_RS28085 (MRY70_28085)	-	6317100..6317693 (+)	594	WP_009615234.1	YggT family protein	-
PM1_RS28090 (MRY70_28090)	-	6317693..6317989 (+)	297	WP_009615233.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42685.06 Da
• Isoelectric Point: 6.7792

>NTDB_id=669110 PM1_RS28065 WP_009615238.1 6313207..6314352(-) (pilU) [Pseudomonas sp. M1]
MEFEKLLRLMVEKGASDLFVTAGVPPSMKVNGKVLPVTKTPLSPEQTRETVLAVMNEQQRRDFAEHHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIEDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDTFEVALKNTLRQAPDVIMIGEVRSRETMEHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHQQIWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSSMTQGLTLELSEDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=669110 PM1_RS28065 WP_009615238.1 6313207..6314352(-) (pilU) [Pseudomonas sp. M1]
ATGGAATTCGAGAAGCTTCTGCGGCTGATGGTGGAGAAGGGCGCCTCGGACCTCTTCGTCACCGCCGGCGTGCCCCCGTC
GATGAAGGTCAACGGCAAGGTGCTGCCGGTGACCAAGACCCCGCTGTCGCCCGAGCAGACCCGCGAGACGGTGCTGGCGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGCACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGGCGC
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGTTGCGCCGCATCGAGACCAACATCCCGACCATCGA
GGACCTCAAGCTGCCGGAGATCCTCAAGAAACTGGCCATGACCAAGCGCGGGCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACACCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGAGCATG
CCGTGGCCTTTGCCGAGACCGGTCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCACCAGCAGATCTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAATACTCCGCTGGCCGCTGACCTGA
TCCGCAAGGGTGAGGTGCATGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTATCAGCTCTACTCCCAGGGCGAGATCACCTATGAAGATGCCCTGGCCTATGCCGACTCGGCCAACGACCTGCGGCT
GATGATCAAGCTCGGGTCGGAGACCGATGCCGACCACCTGTCGAGCATGACTCAGGGGCTGACCCTGGAGCTGAGCGAAG
ACGATCCGAATCGCCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

3D structure

• AlphaFold DB: A0A1A5D0E6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	87.664	100	0.877
pilU	Acinetobacter baylyi ADP1	63.032	98.688	0.622
pilU	Vibrio cholerae strain A1552	60.571	91.864	0.556
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	88.976	0.386