Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JCM16456_RS02070 Genome accession   NZ_AP014635
Coordinates   445557..446594 (+) Length   345 a.a.
NCBI ID   WP_068711930.1    Uniprot ID   -
Organism   Vibrio tritonius strain AM2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440557..451594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JCM16456_RS02040 hemW 441095..442276 (-) 1182 WP_068711918.1 radical SAM family heme chaperone HemW -
  JCM16456_RS02045 - 442276..442875 (-) 600 WP_068711920.1 XTP/dITP diphosphatase -
  JCM16456_RS02050 yggU 443034..443324 (-) 291 WP_068711922.1 DUF167 family protein YggU -
  JCM16456_RS02055 - 443324..443881 (-) 558 WP_068711924.1 YggT family protein -
  JCM16456_RS02060 proC 443952..444770 (-) 819 WP_068711926.1 pyrroline-5-carboxylate reductase -
  JCM16456_RS02065 - 444824..445531 (-) 708 WP_068711928.1 YggS family pyridoxal phosphate-dependent enzyme -
  JCM16456_RS02070 pilT 445557..446594 (+) 1038 WP_068711930.1 type IV pilus twitching motility protein PilT Machinery gene
  JCM16456_RS02075 pilU 446605..447714 (+) 1110 WP_068711932.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JCM16456_RS02080 ruvX 447771..448193 (-) 423 WP_068711934.1 Holliday junction resolvase RuvX -
  JCM16456_RS02085 - 448274..448837 (-) 564 WP_068715843.1 YqgE/AlgH family protein -
  JCM16456_RS02090 gshB 448878..449834 (-) 957 WP_068711936.1 glutathione synthase -
  JCM16456_RS02095 rsmE 449849..450580 (-) 732 WP_068711938.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JCM16456_RS02100 - 450666..451373 (-) 708 WP_068711940.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38089.57 Da        Isoelectric Point: 6.4422

>NTDB_id=66396 JCM16456_RS02070 WP_068711930.1 445557..446594(+) (pilT) [Vibrio tritonius strain AM2]
MDIAELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHAEVHRLVFEIMNDAQRRDYEENLEVDFSFELPNVGR
FRVNAFHQSRGGSAVFRTIPTEVPTLAQLNAPEIFQKMTQYEKGLVLVTGPTGSGKSTTLAAMINEVNTTQNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGADKGMVRSMLSESLRAVIAQKLLKKVGGGRVACHEIMLGTPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMSNGLVELSEVQAKIESDMANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=66396 JCM16456_RS02070 WP_068711930.1 445557..446594(+) (pilT) [Vibrio tritonius strain AM2]
ATGGATATCGCCGAATTACTCGATTTTAGTGTAAAGCATAATGCCTCTGATCTACACCTTTCTGCAGGTGTGCCTCCAAT
GGTCAGAATTGATGGCGAAGTCAGAAAACTAGGTGTTCCTGCGTTTTCTCATGCGGAAGTTCACCGTCTGGTCTTTGAGA
TCATGAACGATGCCCAAAGGCGTGACTACGAAGAGAATCTTGAAGTCGACTTCTCTTTTGAACTACCTAATGTCGGCCGT
TTTCGTGTTAATGCTTTTCATCAATCTCGAGGTGGTTCCGCTGTTTTTCGGACCATTCCTACCGAAGTTCCTACCTTAGC
ACAATTAAATGCCCCTGAGATCTTTCAAAAAATGACTCAGTATGAAAAAGGGTTAGTGTTGGTAACAGGACCAACTGGTT
CAGGGAAGTCCACTACATTGGCCGCAATGATTAACGAAGTGAATACAACACAAAATAAACATATTTTAACGATTGAAGAC
CCGATTGAATTTGTGCACACCAATAATAAGTGTTTGATTAACCAACGTGAGGTGCACAGGGATACCCATAGTTTTAAAGC
GGCTTTACGTTCGGCATTGCGTGAAGATCCTGATGTTATTTTGGTTGGGGAGTTGCGCGATCAAGAAACCATTAGTTTGG
CTTTAACCGCTGCAGAAACTGGGCATTTAGTGTTTGGCACGTTACACACCAGTTCTGCTGCAAAAACCATTGATAGGATT
ATCGACGTATTTCCTGGGGCGGATAAAGGCATGGTACGTTCAATGCTATCCGAGTCGTTACGTGCTGTAATTGCACAAAA
GTTATTGAAAAAAGTCGGTGGTGGGCGAGTGGCTTGTCATGAAATTATGCTGGGCACTCCTGCTATTCGTAATCTGATTC
GCGAAGATAAAGTTGCTCAGATGTATTCAATTATTCAAACCGGTGCGGCACATGGTATGCAAACCATGGAACAAAATGCG
CGCCAATTGATGTCGAATGGGCTTGTCGAATTATCCGAAGTTCAAGCTAAAATCGAATCAGATATGGCTAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.826

100

0.878
  pilT Vibrio cholerae strain A1552

87.826

100

0.878
  pilT Acinetobacter baylyi ADP1

70

98.551

0.69
  pilT Acinetobacter nosocomialis M2

72.036

95.362

0.687
  pilT Acinetobacter baumannii strain A118

71.733

95.362

0.684
  pilT Acinetobacter baumannii D1279779

71.733

95.362

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Pseudomonas aeruginosa PAK

67.941

98.551

0.67
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

65.758

95.652

0.629
  pilT Neisseria gonorrhoeae MS11

65.455

95.652

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.516

92.174

0.484
  pilU Vibrio cholerae strain A1552

41.739

100

0.417
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.4
  pilU Acinetobacter baylyi ADP1

39.375

92.754

0.365

Multiple sequence alignment