Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JCM16456_RS02075 Genome accession   NZ_AP014635
Coordinates   446605..447714 (+) Length   369 a.a.
NCBI ID   WP_068711932.1    Uniprot ID   -
Organism   Vibrio tritonius strain AM2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441605..452714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JCM16456_RS02045 - 442276..442875 (-) 600 WP_068711920.1 XTP/dITP diphosphatase -
  JCM16456_RS02050 yggU 443034..443324 (-) 291 WP_068711922.1 DUF167 family protein YggU -
  JCM16456_RS02055 - 443324..443881 (-) 558 WP_068711924.1 YggT family protein -
  JCM16456_RS02060 proC 443952..444770 (-) 819 WP_068711926.1 pyrroline-5-carboxylate reductase -
  JCM16456_RS02065 - 444824..445531 (-) 708 WP_068711928.1 YggS family pyridoxal phosphate-dependent enzyme -
  JCM16456_RS02070 pilT 445557..446594 (+) 1038 WP_068711930.1 type IV pilus twitching motility protein PilT Machinery gene
  JCM16456_RS02075 pilU 446605..447714 (+) 1110 WP_068711932.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JCM16456_RS02080 ruvX 447771..448193 (-) 423 WP_068711934.1 Holliday junction resolvase RuvX -
  JCM16456_RS02085 - 448274..448837 (-) 564 WP_068715843.1 YqgE/AlgH family protein -
  JCM16456_RS02090 gshB 448878..449834 (-) 957 WP_068711936.1 glutathione synthase -
  JCM16456_RS02095 rsmE 449849..450580 (-) 732 WP_068711938.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JCM16456_RS02100 - 450666..451373 (-) 708 WP_068711940.1 endonuclease -
  JCM16456_RS02105 - 451468..451698 (+) 231 WP_068711941.1 hypothetical protein -
  JCM16456_RS02110 - 451723..452223 (-) 501 WP_068711942.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41511.05 Da        Isoelectric Point: 5.1761

>NTDB_id=66397 JCM16456_RS02075 WP_068711932.1 446605..447714(+) (pilU) [Vibrio tritonius strain AM2]
MFLEQCLSGMITRKASDVYITVGAPVLYRVNGELQGDGEKLTFSDVNNLLNRMMDSEQVEQFYHTHEANFAIVRDSGRFR
VSAFFQREQPGAVIRRIETRIPTFDELHLPELLKDLVMSKRGLVLVVGSTGSGKSTTMAAMTGYRNKNSSGHILTVEDPI
EFVHEHQGCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKEQKDQFLFDLSMNLRGVVGQQLVRDSQGQGRYGAFEILLNTPRVSDLIRRGELHELKETMARSRESGMQTFDQALY
ELVIENKISEEDAMQCADSANDLRLMLKTQRGDTSSGTGSLDHVTIDMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=66397 JCM16456_RS02075 WP_068711932.1 446605..447714(+) (pilU) [Vibrio tritonius strain AM2]
ATGTTTCTGGAACAATGTTTAAGTGGAATGATTACTCGTAAAGCCTCTGATGTGTATATCACTGTCGGAGCTCCAGTGTT
GTATCGAGTGAATGGTGAACTGCAAGGGGATGGTGAAAAACTCACATTTTCGGATGTGAATAATTTGCTTAACCGGATGA
TGGACAGTGAGCAAGTCGAGCAGTTTTACCATACCCATGAAGCAAACTTTGCGATTGTGCGCGATAGTGGGCGTTTTCGT
GTGAGTGCTTTTTTTCAACGCGAGCAACCCGGAGCAGTGATTCGCCGTATAGAAACGCGTATACCTACCTTTGATGAGCT
GCACTTACCTGAATTACTAAAAGATTTAGTGATGAGTAAGCGTGGTTTGGTACTGGTTGTTGGATCTACGGGATCGGGTA
AATCAACAACGATGGCAGCCATGACTGGTTACCGTAATAAAAACTCATCTGGACATATTCTGACGGTGGAAGATCCGATT
GAATTTGTGCATGAACACCAAGGATGTATCGTGACTCAGCGTGAAGTGGGGCTAGATACAGATAGTTACGAAGTGGCACT
AAAAAATTCGCTTCGCCAAGCGCCTGATATGATTCTTATTGGTGAAATTCGTTCGCGTGAAACCATGGAATACGCTATGA
ATTTTGCCGAAACAGGTCATTTATGCATGGCAACGCTGCACGCCAACAATGCTAACCAAGCATTAGAGCGCATTTTACAT
CTGGTGCCTAAAGAGCAAAAAGATCAGTTTTTGTTTGATTTATCGATGAATTTGCGTGGTGTGGTTGGTCAACAATTGGT
TCGTGATAGCCAAGGGCAAGGACGTTACGGTGCGTTTGAGATTTTGCTAAATACACCGCGAGTGTCGGATTTGATTCGTC
GCGGAGAGCTTCACGAGCTGAAAGAAACTATGGCTCGATCTCGTGAATCGGGAATGCAGACCTTTGATCAAGCTTTGTAT
GAATTGGTCATTGAGAATAAGATCTCTGAAGAAGATGCAATGCAATGTGCTGATTCAGCCAATGATCTGCGCTTAATGCT
AAAAACCCAGCGAGGGGATACAAGCAGCGGAACCGGCTCGTTAGATCATGTCACCATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

76.965

100

0.77
  pilU Pseudomonas stutzeri DSM 10701

58

94.851

0.55
  pilU Acinetobacter baylyi ADP1

55.84

95.122

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.813

88.618

0.379
  pilT Legionella pneumophila strain ERS1305867

41.003

91.87

0.377
  pilT Legionella pneumophila strain Lp02

41.003

91.87

0.377
  pilT Pseudomonas aeruginosa PAK

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366
  pilT Acinetobacter baumannii D1279779

39.528

91.87

0.363
  pilT Acinetobacter baumannii strain A118

39.528

91.87

0.363
  pilT Acinetobacter nosocomialis M2

39.528

91.87

0.363

Multiple sequence alignment