Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MNO14_RS03970 Genome accession   NZ_CP093340
Coordinates   884216..885253 (+) Length   345 a.a.
NCBI ID   WP_241945485.1    Uniprot ID   -
Organism   Luteimonas sp. S4-F44     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 879216..890253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO14_RS03940 (MNO14_03940) - 879818..880378 (-) 561 WP_241945479.1 shikimate kinase -
  MNO14_RS03945 (MNO14_03945) - 880446..881255 (-) 810 WP_241945480.1 kinase -
  MNO14_RS03950 (MNO14_03950) pdxH 881345..881953 (+) 609 WP_241945481.1 pyridoxamine 5'-phosphate oxidase -
  MNO14_RS03955 (MNO14_03955) - 882070..882687 (+) 618 WP_241945482.1 NAD(P)-dependent oxidoreductase -
  MNO14_RS03960 (MNO14_03960) - 882743..883213 (-) 471 WP_241945483.1 DUF4426 domain-containing protein -
  MNO14_RS03965 (MNO14_03965) proC 883206..884048 (-) 843 WP_241945484.1 pyrroline-5-carboxylate reductase -
  MNO14_RS03970 (MNO14_03970) pilT 884216..885253 (+) 1038 WP_241945485.1 type IV pilus twitching motility protein PilT Machinery gene
  MNO14_RS03975 (MNO14_03975) pilU 885353..886483 (+) 1131 WP_241945486.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MNO14_RS03980 (MNO14_03980) - 886589..887368 (+) 780 WP_241945487.1 M48 family metallopeptidase -
  MNO14_RS03985 (MNO14_03985) - 887529..888155 (+) 627 WP_183427425.1 superoxide dismutase -
  MNO14_RS03990 (MNO14_03990) - 888253..888792 (-) 540 WP_241945488.1 DNA-3-methyladenine glycosylase I -
  MNO14_RS03995 (MNO14_03995) - 888892..889347 (+) 456 WP_241945489.1 peptidylprolyl isomerase -
  MNO14_RS04000 (MNO14_04000) - 889491..890045 (+) 555 WP_241945490.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38411.08 Da        Isoelectric Point: 6.7269

>NTDB_id=663804 MNO14_RS03970 WP_241945485.1 884216..885253(+) (pilT) [Luteimonas sp. S4-F44]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALIYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLDDLNCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAAMLDHINKNEYAHILSVED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKAMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQQSGMMTLDQHL
QDLVRRGVVARAQAKENAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=663804 MNO14_RS03970 WP_241945485.1 884216..885253(+) (pilT) [Luteimonas sp. S4-F44]
ATGGACATCGCCGAACTGTTGGCGTTCTCGGTCAAGAACAAGGCCTCCGACCTGCATCTTTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGTATCAACATTCCCGCGCTCGACCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAGGAGTTCCTCGAGGTCGACTTCTCGTTCGAGATCCCCGGACTGGCGCGG
TTCCGCGTCAACGCGTTCAATCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACGATTCCGTCCGAGGTGCTGACTCTTGA
CGACCTCAACTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGCTCGACCACATCAACAAGAACGAGTACGCGCACATCCTGTCGGTCGAGGAT
CCGATCGAGTTCGTGCACACCTCGCAGAAATGCCTGGTCAACCAGCGCGAAGTCCACCGCGACACCCACGGCTTCAACGA
GGCACTGCGCTCGGCGCTGCGCGAGGACCCCGATTACATCCTGGTCGGCGAGTTGCGCGACCTGGAGACGATCCGTCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACTCTGCACACCAGTTCGGCGGCAAAGACCATCGACCGCATC
ATCGACGTCTTTCCGGCCGGGGAGAAGGCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCCCAGGC
GCTGCTCAAGAAGGTCGGCGGGGGCCGCACCGCGGCGTGGGAGATCATGGTAGGCATCCCGGCGATCCGGAACCTGATCC
GCGAGGACAAAGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGTCGGGCATGATGACCCTTGACCAGCATCTC
CAGGACCTGGTCAGGCGCGGCGTCGTCGCGCGCGCTCAGGCGAAGGAAAATGCGAAGGACAAGCGACTTTTCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

64.809

98.841

0.641
  pilT Neisseria gonorrhoeae MS11

64.516

98.841

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.406

100

0.484
  pilU Pseudomonas stutzeri DSM 10701

39.08

100

0.394
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38