Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MNO14_RS03975 Genome accession   NZ_CP093340
Coordinates   885353..886483 (+) Length   376 a.a.
NCBI ID   WP_241945486.1    Uniprot ID   -
Organism   Luteimonas sp. S4-F44     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 880353..891483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO14_RS03945 (MNO14_03945) - 880446..881255 (-) 810 WP_241945480.1 kinase -
  MNO14_RS03950 (MNO14_03950) pdxH 881345..881953 (+) 609 WP_241945481.1 pyridoxamine 5'-phosphate oxidase -
  MNO14_RS03955 (MNO14_03955) - 882070..882687 (+) 618 WP_241945482.1 NAD(P)-dependent oxidoreductase -
  MNO14_RS03960 (MNO14_03960) - 882743..883213 (-) 471 WP_241945483.1 DUF4426 domain-containing protein -
  MNO14_RS03965 (MNO14_03965) proC 883206..884048 (-) 843 WP_241945484.1 pyrroline-5-carboxylate reductase -
  MNO14_RS03970 (MNO14_03970) pilT 884216..885253 (+) 1038 WP_241945485.1 type IV pilus twitching motility protein PilT Machinery gene
  MNO14_RS03975 (MNO14_03975) pilU 885353..886483 (+) 1131 WP_241945486.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MNO14_RS03980 (MNO14_03980) - 886589..887368 (+) 780 WP_241945487.1 M48 family metallopeptidase -
  MNO14_RS03985 (MNO14_03985) - 887529..888155 (+) 627 WP_183427425.1 superoxide dismutase -
  MNO14_RS03990 (MNO14_03990) - 888253..888792 (-) 540 WP_241945488.1 DNA-3-methyladenine glycosylase I -
  MNO14_RS03995 (MNO14_03995) - 888892..889347 (+) 456 WP_241945489.1 peptidylprolyl isomerase -
  MNO14_RS04000 (MNO14_04000) - 889491..890045 (+) 555 WP_241945490.1 YqgE/AlgH family protein -
  MNO14_RS04005 (MNO14_04005) ruvX 890042..890527 (+) 486 WP_241945491.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41708.07 Da        Isoelectric Point: 7.0158

>NTDB_id=663805 MNO14_RS03975 WP_241945486.1 885353..886483(+) (pilU) [Luteimonas sp. S4-F44]
MSSLDFTSFLKLMAHQRASDLFITAGLPPSMKVHGKITPITQTPLTPQQARDMVLNVMTPPQREEFERTHECNFAIGVSG
VGRFRISCFYQRNQVGMVLRRIETHIPTIEELNLPAVVKTLAMTKRGIVIMVGGTGAGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHRHAGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMNHAIAFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMDLSLNLKGVIAQQLIPTPDGKGRRAAMEILLGTPLVQDYIREGEIPKLKEIMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSANEVRLRIKLAQGGDARTLAQGLDGVEVAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=663805 MNO14_RS03975 WP_241945486.1 885353..886483(+) (pilU) [Luteimonas sp. S4-F44]
ATGAGCAGCCTCGACTTCACTTCGTTCCTCAAGCTGATGGCCCACCAGCGGGCGTCGGACCTGTTCATCACTGCCGGCCT
GCCGCCGTCGATGAAGGTGCACGGCAAGATCACGCCGATCACCCAGACGCCGCTCACGCCGCAGCAGGCGCGCGACATGG
TGCTCAACGTGATGACGCCGCCGCAGCGCGAGGAGTTCGAGCGCACGCACGAATGCAACTTCGCGATCGGCGTCTCCGGC
GTGGGCCGGTTCCGCATCTCGTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGGCGCATCGAGACCCACATCCC
GACGATCGAGGAACTGAACCTGCCGGCGGTGGTCAAGACGCTGGCGATGACCAAGCGCGGCATCGTGATCATGGTCGGCG
GCACTGGCGCCGGCAAATCGACGTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACG
ATCGAGGATCCGATCGAGTTCGTGCACCGGCACGCCGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGATAG
TTGGGAGGCCGCGCTCAAGAACACCCTGCGCCAGGCCCCCGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGAACCACGCGATCGCCTTCGCCGAAACCGGGCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAATCAGGCGATG
GACCGGGTCATCAACTTCTTCCCCGAGGATCGGCGCAGCCAGTTGCTGATGGACCTGTCGCTCAACCTCAAGGGCGTCAT
CGCCCAGCAATTGATCCCCACGCCCGACGGCAAGGGCCGGCGCGCGGCGATGGAGATCCTGCTCGGGACGCCGCTGGTGC
AGGACTACATCCGCGAAGGCGAGATCCCCAAGCTCAAGGAGATCATGAAAGAGTCGACCAACCTGGGCATGAAGACCTTC
GACCAGTCGTTGTTCGAGCTCTATCAGGCCGGCGAGATCTCCTACGAGGACGCGCTGCGCTACGCCGATTCGGCCAACGA
GGTGCGCCTGCGCATCAAACTCGCCCAGGGCGGCGATGCGCGCACGCTGGCCCAGGGCCTGGATGGGGTGGAAGTCGCCG
AGGTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.302

97.606

0.657
  pilU Acinetobacter baylyi ADP1

62.745

94.947

0.596
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.109

90.691

0.391
  pilT Pseudomonas aeruginosa PAK

42.09

89.096

0.375
  pilT Acinetobacter nosocomialis M2

40.597

89.096

0.362
  pilT Acinetobacter baumannii D1279779

40.597

89.096

0.362
  pilT Acinetobacter baumannii strain A118

40.597

89.096

0.362