Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MNQ95_RS01405 Genome accession   NZ_CP093331
Coordinates   291371..292408 (-) Length   345 a.a.
NCBI ID   WP_162411489.1    Uniprot ID   -
Organism   Pseudoxanthomonas daejeonensis strain S9-A64R     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 286371..297408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNQ95_RS01375 (MNQ95_01375) - 286451..287395 (-) 945 WP_162411494.1 aspartate carbamoyltransferase catalytic subunit -
  MNQ95_RS01380 (MNQ95_01380) ruvX 287513..287986 (-) 474 WP_162411493.1 Holliday junction resolvase RuvX -
  MNQ95_RS01385 (MNQ95_01385) - 287979..288548 (-) 570 WP_162411492.1 YqgE/AlgH family protein -
  MNQ95_RS01390 (MNQ95_01390) - 288627..289187 (+) 561 WP_241953586.1 DNA-3-methyladenine glycosylase I -
  MNQ95_RS01395 (MNQ95_01395) - 289256..289930 (-) 675 WP_162411543.1 YitT family protein -
  MNQ95_RS01400 (MNQ95_01400) pilU 290190..291320 (-) 1131 WP_241953587.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MNQ95_RS01405 (MNQ95_01405) pilT 291371..292408 (-) 1038 WP_162411489.1 type IV pilus twitching motility protein PilT Machinery gene
  MNQ95_RS01410 (MNQ95_01410) - 292522..293202 (+) 681 WP_241953588.1 YggS family pyridoxal phosphate-dependent enzyme -
  MNQ95_RS01415 (MNQ95_01415) proC 293246..294085 (+) 840 WP_241953589.1 pyrroline-5-carboxylate reductase -
  MNQ95_RS01420 (MNQ95_01420) - 294082..294552 (+) 471 WP_241953590.1 DUF4426 domain-containing protein -
  MNQ95_RS01425 (MNQ95_01425) - 294754..295176 (+) 423 WP_162411486.1 hypothetical protein -
  MNQ95_RS01430 (MNQ95_01430) - 295293..296153 (-) 861 WP_202922911.1 pirin family protein -
  MNQ95_RS01435 (MNQ95_01435) - 296272..296937 (-) 666 WP_241953591.1 DUF2306 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38668.34 Da        Isoelectric Point: 6.6395

>NTDB_id=663668 MNQ95_RS01405 WP_162411489.1 291371..292408(-) (pilT) [Pseudoxanthomonas daejeonensis strain S9-A64R]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSDVLTLEDLGCPPIFRELIDQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMMTLDQHL
QDLVKRGLITRHQAKEYARDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=663668 MNQ95_RS01405 WP_162411489.1 291371..292408(-) (pilT) [Pseudoxanthomonas daejeonensis strain S9-A64R]
ATGGATATCGCCGAACTGCTGGCCTTTTCGGTCAAGAACAAGGCTTCCGACCTGCATCTGTCGGCCGGCTTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGCCGGATCAACATCCCGGCGCTGGACCACAAGCAGGTCCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAGGAGTTCCTCGAGGTCGACTTCTCGTTCGAGATCCCGTCCCTGGCCCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGTGGCGCCGGCGCGGTGTTCCGCACCATTCCGTCGGACGTGCTGACCCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCGAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACATCAACAAGAACGAGTACGGGCACATCCTCACCGTCGAGGAC
CCGATCGAGTTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAGGACCCCGACTACATCCTGGTCGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCATACCTCCAGCGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAGAAGCCGATGGTCCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
CCTGCTGAAGAAGGTCGGCGGCGGGCGTACCGCGGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGTCGATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGTGGCCTGATCACGCGCCACCAGGCCAAGGAATACGCCAGGGACAAGCGCCTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.501
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397