Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MNQ95_RS01400 Genome accession   NZ_CP093331
Coordinates   290190..291320 (-) Length   376 a.a.
NCBI ID   WP_241953587.1    Uniprot ID   -
Organism   Pseudoxanthomonas daejeonensis strain S9-A64R     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 285190..296320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNQ95_RS01375 (MNQ95_01375) - 286451..287395 (-) 945 WP_162411494.1 aspartate carbamoyltransferase catalytic subunit -
  MNQ95_RS01380 (MNQ95_01380) ruvX 287513..287986 (-) 474 WP_162411493.1 Holliday junction resolvase RuvX -
  MNQ95_RS01385 (MNQ95_01385) - 287979..288548 (-) 570 WP_162411492.1 YqgE/AlgH family protein -
  MNQ95_RS01390 (MNQ95_01390) - 288627..289187 (+) 561 WP_241953586.1 DNA-3-methyladenine glycosylase I -
  MNQ95_RS01395 (MNQ95_01395) - 289256..289930 (-) 675 WP_162411543.1 YitT family protein -
  MNQ95_RS01400 (MNQ95_01400) pilU 290190..291320 (-) 1131 WP_241953587.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MNQ95_RS01405 (MNQ95_01405) pilT 291371..292408 (-) 1038 WP_162411489.1 type IV pilus twitching motility protein PilT Machinery gene
  MNQ95_RS01410 (MNQ95_01410) - 292522..293202 (+) 681 WP_241953588.1 YggS family pyridoxal phosphate-dependent enzyme -
  MNQ95_RS01415 (MNQ95_01415) proC 293246..294085 (+) 840 WP_241953589.1 pyrroline-5-carboxylate reductase -
  MNQ95_RS01420 (MNQ95_01420) - 294082..294552 (+) 471 WP_241953590.1 DUF4426 domain-containing protein -
  MNQ95_RS01425 (MNQ95_01425) - 294754..295176 (+) 423 WP_162411486.1 hypothetical protein -
  MNQ95_RS01430 (MNQ95_01430) - 295293..296153 (-) 861 WP_202922911.1 pirin family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41950.28 Da        Isoelectric Point: 6.8287

>NTDB_id=663667 MNQ95_RS01400 WP_241953587.1 290190..291320(-) (pilU) [Pseudoxanthomonas daejeonensis strain S9-A64R]
MSSIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIILFVGATGTGKSTSLASMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDTWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKIKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=663667 MNQ95_RS01400 WP_241953587.1 290190..291320(-) (pilU) [Pseudoxanthomonas daejeonensis strain S9-A64R]
ATGAGCAGCATCGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTCCACGGCAAGATCAGCCCGATCACGCAGACGCCGCTCACCCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCGATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTGTCGTGCTTCTACCAGCGCAACCAGGTGGGCATGGTCCTGCGCCGGATCGAGACCAAGATCCC
GACGGTCGAAGAACTGAACCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTGGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACCAGAATTCGACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAC
CTGGGAGAACGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCGATCTCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCATGCGAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGTCGCAGCCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTTGT
AGCGCAGCAGTTGATCCCGACGCCGGACGGCAAGGGGCGCCGGGTGGCGATGGAGATCCTGCTTGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGATCAAGGAAGTGATGAAGGAGTCGACCAACCTCGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCTCCTACGAGGATGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCCCAGGGTGGCGATGCGCGGACCCTGGCGCAGGGGCTGGACGGCGTGGAGATCGCCG
AGGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

64.986

94.947

0.617
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.137

94.947

0.41
  pilT Acinetobacter baumannii strain A118

40.698

91.489

0.372
  pilT Acinetobacter nosocomialis M2

40.698

91.489

0.372
  pilT Acinetobacter baumannii D1279779

40.698

91.489

0.372
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364