Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AS4_RS04555 Genome accession   NZ_AP014630
Coordinates   985425..986462 (-) Length   345 a.a.
NCBI ID   WP_004722735.1    Uniprot ID   A0A077KW48
Organism   Acinetobacter guillouiae strain NBRC 110550     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 980425..991462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS4_RS04525 (AS4_09050) - 981090..981545 (+) 456 WP_004819397.1 bacteriohemerythrin -
  AS4_RS04530 (AS4_09060) - 981749..982831 (+) 1083 WP_227548124.1 hypothetical protein -
  AS4_RS04535 (AS4_09070) - 982840..983169 (+) 330 WP_167468929.1 RnfH family protein -
  AS4_RS04540 (AS4_09080) - 983254..983652 (-) 399 WP_004722739.1 outer membrane protein assembly factor BamE -
  AS4_RS04545 (AS4_09090) fur 983765..984202 (+) 438 WP_004722737.1 ferric iron uptake transcriptional regulator -
  AS4_RS04550 (AS4_09100) pilU 984281..985399 (-) 1119 WP_004722736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AS4_RS04555 (AS4_09110) pilT 985425..986462 (-) 1038 WP_004722735.1 type IV pilus twitching motility protein PilT Machinery gene
  AS4_RS04560 (AS4_09120) - 986586..987278 (+) 693 WP_096733349.1 YggS family pyridoxal phosphate-dependent enzyme -
  AS4_RS04565 (AS4_09130) - 987401..988234 (+) 834 WP_096733350.1 undecaprenyl-diphosphate phosphatase -
  AS4_RS04570 (AS4_09140) - 988439..989242 (+) 804 WP_096733351.1 thioesterase family protein -
  AS4_RS04575 (AS4_09150) - 989538..990329 (+) 792 WP_096733352.1 hypothetical protein -
  AS4_RS04580 (AS4_09160) - 990497..991252 (+) 756 WP_096733353.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38286.02 Da        Isoelectric Point: 6.5057

>NTDB_id=66364 AS4_RS04555 WP_004722735.1 985425..986462(-) (pilT) [Acinetobacter guillouiae strain NBRC 110550]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHRLVYDIMNDKQRRDFEEQLETDFSFEVPGIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDLCELPRGLILVTGPTGSGKSTTLASMIDFINDSRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVAKGVISPQVARNVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=66364 AS4_RS04555 WP_004722735.1 985425..986462(-) (pilT) [Acinetobacter guillouiae strain NBRC 110550]
ATGGATATTACAGAATTATTGGCGTTTTCCGTCAAAAACGGTGCATCTGACTTACACCTTTCAGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAGGTCCGTCGTATCAATTTACCAGCATTGGAACATAAAGAAGTTCACAGACTGGTTTATGACA
TCATGAATGATAAACAACGTCGTGATTTTGAAGAACAATTAGAAACTGACTTCTCATTTGAAGTTCCAGGTATTGCCCGT
TTCCGTGTCAACGCATTCAATCAAAACCGTGGTGCGGGTGCTGTATTTCGTACCATTCCTTCTAAAGTATTGACCATGGA
AGATTTAGGTTTAGGACAAATCTTTAAAGATCTTTGTGAATTACCCCGTGGTTTGATCCTTGTAACAGGTCCAACAGGTT
CCGGTAAATCAACCACATTGGCCTCTATGATTGACTTTATTAATGACAGTCGCTATGACCATATTTTAACGGTCGAAGAT
CCAATCGAATTTGTACACCAATCTAAAAAGTGTCTCATCAACCAGCGTGAAGTGCATCGTGATACACATGGCTTTAATGA
AGCACTGCGTTCTGCGTTACGTGAAGACCCTGACATTATCTTGGTTGGTGAGATGCGTGACCTTGAAACCATTCGCCTTG
CTTTAACAGCAGCAGAAACAGGTCACTTGGTTTTCGGTACACTACACACCACATCAGCGGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTCCGTGCCATGTTATCAGAATCATTACAAGCGGTTATTTCACAAAC
CCTACTGAAAAAGAATGGTGGCGGACGAGTTGCAGCACACGAAATCATGATCGGTATTCCTGCAATTCGTAACTTAATCC
GTGAAAACAAAGTTGCACAGATGTATTCAGCAATTCAGACCGGTGCAAACTATGGTATGACCACATTGGATCAGAGTTTA
AAAACGCTGGTCGCCAAAGGTGTTATTAGTCCTCAAGTTGCAAGAAATGTAGCAAAACAACCAGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A077KW48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

93.913

100

0.939
  pilT Acinetobacter baumannii D1279779

93.913

100

0.939
  pilT Acinetobacter nosocomialis M2

93.623

100

0.936
  pilT Acinetobacter baylyi ADP1

86.628

99.71

0.864
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

82.849

99.71

0.826
  pilT Legionella pneumophila strain Lp02

75.444

97.971

0.739
  pilT Legionella pneumophila strain ERS1305867

75.444

97.971

0.739
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.277

96.232

0.667
  pilT Vibrio cholerae strain A1552

69.277

96.232

0.667
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Acinetobacter baylyi ADP1

39.884

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.39

95.942

0.397

Multiple sequence alignment