Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AS4_RS04550 Genome accession   NZ_AP014630
Coordinates   984281..985399 (-) Length   372 a.a.
NCBI ID   WP_004722736.1    Uniprot ID   A0A077KUG3
Organism   Acinetobacter guillouiae strain NBRC 110550     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 979281..990399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS4_RS04525 (AS4_09050) - 981090..981545 (+) 456 WP_004819397.1 bacteriohemerythrin -
  AS4_RS04530 (AS4_09060) - 981749..982831 (+) 1083 WP_227548124.1 hypothetical protein -
  AS4_RS04535 (AS4_09070) - 982840..983169 (+) 330 WP_167468929.1 RnfH family protein -
  AS4_RS04540 (AS4_09080) - 983254..983652 (-) 399 WP_004722739.1 outer membrane protein assembly factor BamE -
  AS4_RS04545 (AS4_09090) fur 983765..984202 (+) 438 WP_004722737.1 ferric iron uptake transcriptional regulator -
  AS4_RS04550 (AS4_09100) pilU 984281..985399 (-) 1119 WP_004722736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AS4_RS04555 (AS4_09110) pilT 985425..986462 (-) 1038 WP_004722735.1 type IV pilus twitching motility protein PilT Machinery gene
  AS4_RS04560 (AS4_09120) - 986586..987278 (+) 693 WP_096733349.1 YggS family pyridoxal phosphate-dependent enzyme -
  AS4_RS04565 (AS4_09130) - 987401..988234 (+) 834 WP_096733350.1 undecaprenyl-diphosphate phosphatase -
  AS4_RS04570 (AS4_09140) - 988439..989242 (+) 804 WP_096733351.1 thioesterase family protein -
  AS4_RS04575 (AS4_09150) - 989538..990329 (+) 792 WP_096733352.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41869.16 Da        Isoelectric Point: 6.2461

>NTDB_id=66363 AS4_RS04550 WP_004722736.1 984281..985399(-) (pilU) [Acinetobacter guillouiae strain NBRC 110550]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLNGEIIMQLLNSIMSEKQRKEFAETRECNFAITNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNSNSKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=66363 AS4_RS04550 WP_004722736.1 984281..985399(-) (pilU) [Acinetobacter guillouiae strain NBRC 110550]
ATGGACTTTAATGACTTGCTCAATATGATGATTGAGCAAAAAGCATCAGACTTATTTATTACCGCCGATGTTGAACCATC
AATGAAAATTAATGGTCAAATTGTTCCTGTGGTAAAAACCAAGTTAAACGGCGAAATTATTATGCAATTGCTTAACTCTA
TTATGAGTGAAAAGCAGCGTAAAGAATTTGCTGAGACGCGTGAATGTAACTTTGCCATTACCAATCGCGATAAAACTGCG
CGTTTCCGTGTCAGCGCTTTTCAACAACGTGATATGCCTGGAATGGTTTTGCGTCGTATTGAAACTCAAATTCCGACTAT
GGATGAGCTTAAATTACCGCCTATTCTTAAAGATCTCGCAATGACCAAACGTGGGATCATCATCTTTGTTGGTGCAACAG
GGACAGGTAAATCAACCTCGCTTGCATCCATCATTGGTTATCGCAATCAAAATTCTAAAGGTCATATCATCACCATTGAA
GACCCAATTGAATTTGTACACGAACATGCAGGATGTATTATTACCCAACGTGAAGTGGGAATTGATACAGATTCTTTTGA
AATTGCGCTTAAGAATACTTTGCGTCAAGCACCAGATGTGATCTTGATTGGTGAGATCCGTTCTCGTGAAACCATGGACT
ATGCCATTGCCTTTGCTGAAACAGGTCACTTAGTATTTGCCACACTACATGCCAACAATGCCAACCAAGCAATCGACCGG
ATTATTCACTTCTTTGAGTCTGACCGTCATAGCCAATTGTTTATGGATTTATCGCTTAACCTCAAAGCCATGGTTGCACA
GCAATTGATTCCAACACCAGACGGTAATTCTCGTCGTGCTGCAATTGAAATTCTCATCAACTCACCCTTAGTTTCAGATT
TAATCCGTAAAGGTGATGTACATGAAATTAAGGATTTGATGAAGCGCTCTCGTGAATTGGGTATGCAGACATTTGACCAA
GCTTTATTTGACCTCTATAAAGCGGGACAAATTACCTATAAAGACGCACTTAAACATGCCGACTCTCCAAATGATTTACG
TCTACAAATCAAATTGGATGAAGAAGGTGGTGCACAACTACTCAATTCAAATAGCAAACTCACCTTTGATGGACAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A077KUG3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

69.126

98.387

0.68
  pilU Vibrio cholerae strain A1552

54.595

99.462

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.353

93.011

0.403
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Acinetobacter baumannii D1279779

39.65

92.204

0.366
  pilT Acinetobacter baylyi ADP1

39.65

92.204

0.366
  pilT Acinetobacter nosocomialis M2

39.65

92.204

0.366
  pilT Acinetobacter baumannii strain A118

39.65

92.204

0.366
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Neisseria meningitidis 8013

39.412

91.398

0.36
  pilT Neisseria gonorrhoeae MS11

39.412

91.398

0.36

Multiple sequence alignment