Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LIW09_RS02170 Genome accession   NZ_CP093311
Coordinates   479857..480894 (+) Length   345 a.a.
NCBI ID   WP_256646341.1    Uniprot ID   -
Organism   Thermomonas paludicola strain IMCC34681     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 474857..485894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIW09_RS02140 - 475726..476271 (-) 546 WP_256646337.1 shikimate kinase -
  LIW09_RS02145 pdxH 476327..476923 (+) 597 WP_256646338.1 pyridoxamine 5'-phosphate oxidase -
  LIW09_RS02150 - 476932..477729 (+) 798 WP_256647123.1 cobalamin-binding protein -
  LIW09_RS02155 - 477717..478199 (-) 483 WP_256646339.1 DUF4426 domain-containing protein -
  LIW09_RS02160 proC 478210..479028 (-) 819 WP_256646340.1 pyrroline-5-carboxylate reductase -
  LIW09_RS02165 - 479025..479702 (-) 678 WP_256647124.1 YggS family pyridoxal phosphate-dependent enzyme -
  LIW09_RS02170 pilT 479857..480894 (+) 1038 WP_256646341.1 type IV pilus twitching motility protein PilT Machinery gene
  LIW09_RS02175 pilU 480919..482049 (+) 1131 WP_256646342.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LIW09_RS02180 - 482089..482655 (+) 567 WP_256646343.1 YqgE/AlgH family protein -
  LIW09_RS02185 ruvX 482652..483149 (+) 498 WP_256646344.1 Holliday junction resolvase RuvX -
  LIW09_RS02190 - 483182..484117 (+) 936 WP_256646345.1 aspartate carbamoyltransferase catalytic subunit -
  LIW09_RS02195 - 484149..484532 (+) 384 WP_256646346.1 YbjQ family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38547.23 Da        Isoelectric Point: 6.7276

>NTDB_id=663461 LIW09_RS02170 WP_256646341.1 479857..480894(+) (pilT) [Thermomonas paludicola strain IMCC34681]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPRIFKELIEQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSIED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPAGEKSMVRSMLSESLRAVIAQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQGQGMQTLDQHL
QELVKRSMVSRQQARDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=663461 LIW09_RS02170 WP_256646341.1 479857..480894(+) (pilT) [Thermomonas paludicola strain IMCC34681]
ATGGATATCGCTGAGCTGCTGGCATTTTCCGTCAAGAACAAGGCGTCGGACTTGCACCTGTCCGCCGGCCTGCCACCGAT
GATTCGCGTGGATGGCGACGTCCGCCGCATCAACATCCCTGCGCTGGACCACAAGCAGGTGCATGCATTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTTCGAGGAATTCCTTGAAGTGGACTTCAGTTTCGAGATTCCCAGTCTGGCGCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGTGGCGCGGGCGCGGTGTTCCGCACCATTCCCTCGGAGGTGCTGACGCTGGA
GGACCTGGGTTGCCCGCGGATCTTCAAGGAACTGATCGAGCAGCCGCAGGGCCTGATCCTGGTCACCGGGCCCACCGGCT
CCGGCAAGTCCACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTGTCGATCGAGGAC
CCGATCGAATTCGTGCATACCTCGCAGAAATGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCACGGGTTCAACGA
AGCGCTGCGCTCGGCGTTGCGTGAAGACCCCGACATTATCCTGGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACGGGCCACCTGGTGTTTGCCACCTTGCATACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGTCGATGGTGCGCTCGATGCTGTCGGAATCGCTGCGCGCGGTGATTGCGCAGGC
GCTGCTGAAGAAGGTGGGGGGCGGCCGTACCGCCGCCTGGGAAATCATGGTCGGCACGCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCGATCCAGACGGGCCAAGGGCAGGGCATGCAGACGCTTGACCAGCACTTG
CAGGAATTGGTCAAGCGCAGCATGGTCAGCCGCCAGCAGGCGCGCGACTATGCCAAGGACAAGCGATTGTTCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

78.171

98.261

0.768
  pilT Acinetobacter baylyi ADP1

76.453

99.71

0.762
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.149

97.101

0.681
  pilT Vibrio cholerae strain A1552

70.149

97.101

0.681
  pilT Neisseria meningitidis 8013

64.809

98.841

0.641
  pilT Neisseria gonorrhoeae MS11

64.516

98.841

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.667

100

0.42
  pilU Vibrio cholerae strain A1552

41.092

100

0.414
  pilU Acinetobacter baylyi ADP1

40.882

98.551

0.403