Detailed information NTG0663462

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LIW09_RS02175
• Genome accession: NZ_CP093311
• Coordinates: 480919..482049 (+)
• Length: 376 a.a.
• NCBI ID: WP_256646342.1
• Uniprot ID: -
• Organism: Thermomonas paludicola strain IMCC34681
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 475919..487049

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LIW09_RS02145	pdxH	476327..476923 (+)	597	WP_256646338.1	pyridoxamine 5'-phosphate oxidase	-
LIW09_RS02150	-	476932..477729 (+)	798	WP_256647123.1	cobalamin-binding protein	-
LIW09_RS02155	-	477717..478199 (-)	483	WP_256646339.1	DUF4426 domain-containing protein	-
LIW09_RS02160	proC	478210..479028 (-)	819	WP_256646340.1	pyrroline-5-carboxylate reductase	-
LIW09_RS02165	-	479025..479702 (-)	678	WP_256647124.1	YggS family pyridoxal phosphate-dependent enzyme	-
LIW09_RS02170	pilT	479857..480894 (+)	1038	WP_256646341.1	type IV pilus twitching motility protein PilT	Machinery gene
LIW09_RS02175	pilU	480919..482049 (+)	1131	WP_256646342.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LIW09_RS02180	-	482089..482655 (+)	567	WP_256646343.1	YqgE/AlgH family protein	-
LIW09_RS02185	ruvX	482652..483149 (+)	498	WP_256646344.1	Holliday junction resolvase RuvX	-
LIW09_RS02190	-	483182..484117 (+)	936	WP_256646345.1	aspartate carbamoyltransferase catalytic subunit	-
LIW09_RS02195	-	484149..484532 (+)	384	WP_256646346.1	YbjQ family protein	-
LIW09_RS02200	mgtE	484575..485936 (-)	1362	WP_256646347.1	magnesium transporter	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 41814.08 Da
• Isoelectric Point: 6.7682

>NTDB_id=663462 LIW09_RS02175 WP_256646342.1 480919..482049(+) (pilU) [Thermomonas paludicola strain IMCC34681]
MSSIDFTSFLKLMAHQKASDLFITAGMPPSMKVNGKVSPITQNPLTSQQSRDLVLNVMTPQQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIVKTLAMTKRGIIIFVGATGTGKSTSLAAMVGYRNANSTGHIIT
IEDPIEFVHKHDGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPDERRSQLLMDLSLNLKGVVAQQLIPTPDGKARRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESIQLGMKTF
DQSLFELYQAGEISYEDALRYADSSNEVRLRIKLAQGGDARTLAQGLDGVEVVEAR

Nucleotide

• Length: 1131 bp

>NTDB_id=663462 LIW09_RS02175 WP_256646342.1 480919..482049(+) (pilU) [Thermomonas paludicola strain IMCC34681]
ATGAGCAGCATCGATTTCACCTCGTTCCTCAAACTGATGGCGCACCAGAAGGCGTCCGACCTGTTCATCACCGCTGGCAT
GCCGCCATCGATGAAGGTGAACGGGAAAGTCAGCCCGATCACCCAGAACCCGCTTACCTCGCAGCAGTCGCGAGATCTGG
TGCTGAACGTGATGACGCCCCAGCAGCGCGAGGAGTTCGAAAAAACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTGGGGCGCTTCCGCGTGAGTTGTTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGCATCCC
GACGGTGGAGGAGCTCAGCCTGCCGCCGATCGTGAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTGGGTG
CCACCGGTACCGGCAAATCCACCTCGCTGGCGGCGATGGTGGGCTACCGCAACGCCAATTCGACCGGCCACATCATCACC
ATCGAGGATCCGATCGAGTTCGTGCACAAGCACGACGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAAAATGCGCTGAAGAACACCCTGCGGCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGATCACGCCATCTCGTTTGCCGAAACCGGCCATCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCCATG
GATCGGATCATCAACTTCTTCCCCGATGAGCGCCGCAGCCAGCTGCTGATGGATCTGTCGCTGAACCTCAAGGGCGTGGT
GGCGCAGCAGCTGATCCCGACGCCGGACGGCAAGGCGCGCCGGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGAGTCCATCCAGCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTATCAGGCGGGCGAGATCAGCTACGAAGATGCGCTGCGCTACGCGGACTCTTCCAACGA
AGTACGCCTGCGCATCAAGCTGGCACAGGGCGGGGATGCCCGCACGCTGGCGCAGGGGCTGGATGGGGTGGAGGTTGTCG
AGGCGCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	67.03	97.606	0.654
pilU	Acinetobacter baylyi ADP1	63.305	94.947	0.601
pilU	Vibrio cholerae strain A1552	54.062	94.947	0.513
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	92.021	0.391
pilT	Pseudomonas aeruginosa PAK	40.896	89.096	0.364