Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MM300_RS02650 Genome accession   NZ_CP093301
Coordinates   509737..510777 (-) Length   346 a.a.
NCBI ID   WP_255245205.1    Uniprot ID   -
Organism   Evansella sp. LMS18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504737..515777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM300_RS02625 (MM300_02605) - 505294..506196 (-) 903 WP_255243666.1 PilN domain-containing protein -
  MM300_RS02630 (MM300_02610) pilM 506196..507188 (-) 993 WP_255243667.1 type IV pilus biogenesis protein PilM -
  MM300_RS23580 comC 507212..507685 (-) 474 WP_369683967.1 A24 family peptidase Machinery gene
  MM300_RS23585 - 507707..507964 (-) 258 Protein_526 prepilin peptidase -
  MM300_RS23550 - 507995..508417 (-) 423 WP_303837773.1 type II secretion system protein -
  MM300_RS02645 (MM300_02625) - 508532..509737 (-) 1206 WP_255243669.1 type II secretion system F family protein -
  MM300_RS02650 (MM300_02630) pilT 509737..510777 (-) 1041 WP_255245205.1 type IV pilus twitching motility protein PilT Machinery gene
  MM300_RS02655 (MM300_02635) - 510787..512448 (-) 1662 WP_255243670.1 GspE/PulE family protein -
  MM300_RS02660 (MM300_02640) - 512737..513171 (+) 435 WP_255243671.1 hypothetical protein -
  MM300_RS02665 (MM300_02645) - 513175..513639 (+) 465 WP_255243672.1 PilW family protein -
  MM300_RS02670 (MM300_02650) - 513651..514790 (+) 1140 WP_255243673.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38150.93 Da        Isoelectric Point: 6.9517

>NTDB_id=663310 MM300_RS02650 WP_255245205.1 509737..510777(-) (pilT) [Evansella sp. LMS18]
MNTGIEKILTEAIHLQASDIHLTIGKPPVLRIHGDLVTVSNETLTPEAMQGLVAEVLPPMMRDHLETAREADFSYGIPGV
ARFRLNAYYQRSHLSMAIRVIPTEIPDLEELQMPAVLRKIAEKKQGLVLVTGPTGSGKSTTQAAMIKHINEHMKKHIVTL
EDPIEYLHKHNNSIIDQREVGFDTRSFAAGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVLGTLHTSSAAATIN
RIVDVFDSSQQPQIRVQLASVLAAIISQRLMPTADRHGRVAATEVLINNSAVANMVRSDKVHQIESILQTSRAEGMHTLE
MSIGHLLKLGKISRDEAQPYIRERNL

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=663310 MM300_RS02650 WP_255245205.1 509737..510777(-) (pilT) [Evansella sp. LMS18]
TTGAATACAGGCATCGAAAAAATACTGACAGAAGCAATTCACCTGCAGGCCTCAGATATTCATCTGACGATAGGTAAGCC
TCCAGTTCTCCGGATTCATGGGGATCTGGTTACTGTAAGCAACGAGACGCTGACACCGGAGGCCATGCAGGGGCTCGTCG
CAGAAGTTCTACCGCCAATGATGAGGGACCATTTGGAAACTGCCCGGGAAGCCGATTTTTCCTACGGTATTCCTGGGGTG
GCAAGGTTCCGGTTAAACGCTTATTATCAGCGGTCACATCTTAGCATGGCTATCAGGGTGATCCCTACGGAAATCCCTGA
CCTTGAGGAACTGCAAATGCCTGCTGTTTTAAGAAAGATTGCCGAGAAAAAACAAGGGCTAGTCCTCGTAACCGGTCCAA
CTGGAAGCGGAAAATCCACAACGCAGGCGGCGATGATTAAACATATTAACGAACATATGAAAAAGCATATTGTTACTCTG
GAAGACCCTATCGAATATCTCCACAAACATAACAACTCGATTATTGACCAGCGGGAAGTGGGCTTTGACACAAGATCATT
TGCCGCAGGATTGAGGGCAGCTCTGAGACAGGACCCTGACGTTATCTTAGTCGGGGAAATGAGAGATCTGGAGACGATTT
CCACAGCCATCACTGCTGCTGAAACAGGGCATCTAGTCTTAGGGACACTTCATACATCAAGTGCAGCGGCAACCATTAAC
CGGATTGTGGACGTGTTCGATTCCAGCCAGCAGCCGCAAATAAGGGTGCAGCTCGCTTCTGTATTAGCAGCGATAATCTC
CCAGCGGCTGATGCCTACAGCTGATCGCCATGGAAGGGTCGCCGCAACGGAAGTATTAATTAACAATTCAGCTGTAGCAA
ACATGGTACGCAGTGATAAAGTGCATCAAATCGAAAGTATCCTGCAGACAAGCAGAGCGGAGGGCATGCACACGCTGGAG
ATGAGTATCGGCCATCTGCTTAAGCTTGGAAAAATATCACGAGATGAAGCGCAGCCTTACATCAGGGAGAGAAATTTATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

97.977

0.488
  pilT Vibrio cholerae strain A1552

48.961

97.399

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.961

97.399

0.477
  pilT Neisseria meningitidis 8013

49.845

93.353

0.465
  pilT Neisseria gonorrhoeae MS11

49.536

93.353

0.462
  pilT Pseudomonas stutzeri DSM 10701

47.305

96.532

0.457
  pilT Pseudomonas aeruginosa PAK

46.407

96.532

0.448
  pilT Acinetobacter baylyi ADP1

46.201

95.087

0.439
  pilT Acinetobacter nosocomialis M2

45.897

95.087

0.436
  pilT Acinetobacter baumannii strain A118

45.897

95.087

0.436
  pilT Acinetobacter baumannii D1279779

45.897

95.087

0.436
  pilT Legionella pneumophila strain ERS1305867

44.745

96.243

0.431
  pilT Legionella pneumophila strain Lp02

44.745

96.243

0.431
  pilU Pseudomonas stutzeri DSM 10701

40.841

96.243

0.393
  pilU Acinetobacter baylyi ADP1

40.248

93.353

0.376
  pilU Vibrio cholerae strain A1552

38.279

97.399

0.373