Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AS19_RS13580 Genome accession   NZ_AP014613
Coordinates   2987550..2988584 (+) Length   344 a.a.
NCBI ID   WP_011589941.1    Uniprot ID   -
Organism   Alcanivorax sp. NBRC 101098     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982550..2993584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS19_RS13560 (AS19_27240) - 2983449..2984912 (-) 1464 WP_046962219.1 sensor domain-containing diguanylate cyclase -
  AS19_RS13565 (AS19_27250) - 2985126..2985674 (-) 549 WP_035459852.1 YggT family protein -
  AS19_RS13570 (AS19_27260) proC 2985895..2986704 (-) 810 WP_011589939.1 pyrroline-5-carboxylate reductase -
  AS19_RS13575 (AS19_27270) - 2986725..2987414 (-) 690 WP_041705640.1 YggS family pyridoxal phosphate-dependent enzyme -
  AS19_RS13580 (AS19_27280) pilT 2987550..2988584 (+) 1035 WP_011589941.1 type IV pilus twitching motility protein PilT Machinery gene
  AS19_RS13585 (AS19_27290) pilU 2988645..2989766 (+) 1122 WP_011589942.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AS19_RS13590 (AS19_27300) - 2989930..2990136 (-) 207 WP_035460127.1 SlyX family protein -
  AS19_RS13595 (AS19_27310) - 2990517..2991071 (+) 555 WP_041705170.1 TMEM165/GDT1 family protein -
  AS19_RS13600 (AS19_27320) - 2991213..2991752 (+) 540 WP_011589945.1 YaeQ family protein -
  AS19_RS13605 (AS19_27330) - 2991859..2992422 (+) 564 WP_011589946.1 HdeD family acid-resistance protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37992.61 Da        Isoelectric Point: 6.1851

>NTDB_id=66221 AS19_RS13580 WP_011589941.1 2987550..2988584(+) (pilT) [Alcanivorax sp. NBRC 101098]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPAMEHKEVHSLIYDIMNDKQRKDFEEFFETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGKVFQNISEMPRGIVLVTGPTGSGKSTTLAAMVDYINDSRYEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKSMIRSMLSESLQAVVSQTLMKKNGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDQCL
QGLVQKGLVARDVAREKAKNPDSL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=66221 AS19_RS13580 WP_011589941.1 2987550..2988584(+) (pilT) [Alcanivorax sp. NBRC 101098]
ATGGATATTACCGAGCTGCTCGCTTTCAGCGCCAAAAACGGCGCCTCTGACCTTCACTTGTCCGCGGGCCTGCCCCCGAT
GATCCGGGTGGATGGCGACGTGCGCCGCATCAACCTGCCCGCCATGGAGCACAAGGAAGTTCATTCATTGATCTATGACA
TCATGAATGACAAGCAGCGCAAAGATTTCGAGGAATTCTTCGAGACGGACTTCTCCTTCGAGGTGCCCGGCGTAGCCCGC
TTCCGGGTAAACGCCTTTAACCACAACCGTGGTGCCGGGGCGGTATTTCGAACCATTCCATCGAAAGTGCTGACCATGGA
AGACTTGGGGATGGGCAAGGTCTTCCAGAATATCTCCGAAATGCCCCGGGGTATCGTGCTAGTCACGGGGCCCACCGGCT
CGGGTAAATCTACCACCCTGGCAGCCATGGTCGATTACATCAACGATTCCCGTTACGAGCACATCCTCACCATTGAGGAT
CCTATCGAATTCGTACACGAATCCAAGAAATGTCTAGTCAACCAGCGCGAAGTTCACCGCGACACCCTCGGTTTCTCTGA
AGCCCTGCGCTCGGCACTGCGGGAAGATCCAGACATTATTTTGGTGGGTGAACTGCGGGATCTGGAAACCATCCGCCTGG
CGCTAACGGCCGCAGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCACCTCCGCGGCTAAAACCATTGACCGAGTA
ATCGACGTGTTCCCCGCCGAAGAAAAATCCATGATCCGCTCAATGCTGTCGGAATCACTGCAGGCGGTGGTGTCACAAAC
GCTGATGAAGAAAAACGGCGGCGGCCGTGTGGCGGCCCACGAAATCATGATGGGCACCCCAGCGATACGGAACCTGATTC
GTGAAGATAAGGTGGCCCAGATGTATTCCGCCATCCAGACCGGCGGTGCATTAGGCATGCAGACGCTGGACCAGTGCCTG
CAAGGGCTGGTGCAGAAAGGCCTGGTGGCCCGTGATGTGGCTCGTGAGAAAGCCAAGAACCCAGATTCTCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

80.523

100

0.805
  pilT Legionella pneumophila strain Lp02

76.765

98.837

0.759
  pilT Legionella pneumophila strain ERS1305867

76.765

98.837

0.759
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.997

95.64

0.66
  pilT Vibrio cholerae strain A1552

68.997

95.64

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.17

99.419

0.509
  pilU Pseudomonas stutzeri DSM 10701

39.71

100

0.398
  pilU Vibrio cholerae strain A1552

41.538

94.477

0.392
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment