NTDB

Overview

Name	pilE	Type	Machinery gene
Locus tag	MJ608_RS18105	Genome accession	NZ_CP092733
Coordinates	3872825..3873250 (-)	Length	141 a.a.
NCBI ID	WP_256629289.1	Uniprot ID	-
Organism	Comamonas thiooxydans strain CHJ01
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3867825..3878250

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MJ608_RS18080 (MJ608_18050)	-	3868000..3868473 (+)	474	WP_034351037.1	hypothetical protein	-
MJ608_RS18085 (MJ608_18055)	-	3868582..3868932 (+)	351	WP_034348032.1	phage holin family protein	-
MJ608_RS18090 (MJ608_18060)	-	3868891..3870552 (-)	1662	WP_256629286.1	M48 family metalloprotease	-
MJ608_RS18095 (MJ608_18065)	moaC	3870753..3871220 (+)	468	WP_256629287.1	cyclic pyranopterin monophosphate synthase MoaC	-
MJ608_RS18100 (MJ608_18070)	-	3871177..3872862 (-)	1686	WP_256631291.1	O-antigen ligase	-
MJ608_RS18105 (MJ608_18075)	pilE	3872825..3873250 (-)	426	WP_256629289.1	pilin	Machinery gene
MJ608_RS18110 (MJ608_18080)	-	3873439..3874158 (-)	720	WP_039050359.1	FHA domain-containing protein	-
MJ608_RS18115 (MJ608_18085)	ssb	3874475..3875023 (-)	549	WP_003064700.1	single-stranded DNA-binding protein	Machinery gene
MJ608_RS18120 (MJ608_18090)	-	3875297..3876469 (-)	1173	WP_039050399.1	MFS transporter	-

Sequence

Protein

Download Length: 141 a.a. Molecular weight: 14459.60 Da Isoelectric Point: 9.3653

                                >NTDB_id=659913 MJ608_RS18105 WP_256629289.1 3872825..3873250(-) (pilE) [Comamonas thiooxydans strain CHJ01]

MKRSIQKGFTLIELMIVVAIIGILAAVALPAYQDYTARAQMSEAFTLASGQKGAVSEYHASNGAFPATNASAGIDGNITG

KYVSDVVVGTSGVITATMKSTGISDGIKGKKITLTPTAGSGSYTWVCGTDALGKYLPSSCR

Nucleotide

Download Length: 426 bp

                                >NTDB_id=659913 MJ608_RS18105 WP_256629289.1 3872825..3873250(-) (pilE) [Comamonas thiooxydans strain CHJ01]

ATGAAGCGTTCCATTCAAAAGGGTTTCACCCTGATCGAACTGATGATCGTTGTGGCGATCATCGGTATTCTGGCTGCCGT

GGCTCTGCCTGCTTATCAGGATTACACCGCGCGTGCTCAAATGAGCGAAGCATTCACGCTTGCTAGCGGTCAGAAAGGTG

CTGTTTCAGAATATCATGCAAGCAATGGTGCATTCCCTGCAACCAACGCATCTGCTGGAATTGATGGAAATATCACTGGC

AAGTATGTCTCTGATGTTGTTGTGGGGACAAGCGGTGTGATTACTGCAACTATGAAGTCGACCGGAATTTCTGATGGAAT

TAAGGGTAAGAAAATTACGCTGACTCCGACAGCTGGTAGCGGATCTTATACATGGGTTTGTGGAACTGATGCACTTGGAA

AGTATTTGCCTTCCTCTTGCCGATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)

Pilin (bacterial filament) (Pilin)

(37-141)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilE	Neisseria gonorrhoeae MS11	53.797	100	0.603
pilE	Neisseria gonorrhoeae strain FA1090	52.532	100	0.589
pilA2	Legionella pneumophila str. Paris	51.449	97.872	0.504
pilA2	Legionella pneumophila strain ERS1305867	51.449	97.872	0.504
comP	Acinetobacter baylyi ADP1	48.299	100	0.504
pilA/pilA1	Eikenella corrodens VA1	43.478	100	0.496
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	35.106	100	0.468
pilA/pilAI	Pseudomonas stutzeri DSM 10701	45.139	100	0.461
pilA	Ralstonia pseudosolanacearum GMI1000	37.126	100	0.44
pilA	Acinetobacter nosocomialis M2	44.853	96.454	0.433
pilA	Pseudomonas aeruginosa PAK	40.268	100	0.426
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.86	100	0.404
pilA	Acinetobacter baumannii strain A118	38.194	100	0.39
pilA	Vibrio cholerae C6706	35.862	100	0.369
pilA	Vibrio cholerae strain A1552	35.862	100	0.369
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	35.862	100	0.369

Detailed information NTG0659913