Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SMCB_RS00605 Genome accession   NZ_AP014569
Coordinates   126739..127782 (-) Length   347 a.a.
NCBI ID   WP_045534322.1    Uniprot ID   -
Organism   Serpentinimonas maccroryi strain B1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 121739..132782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMCB_RS00575 (SMCB_0117) - 121877..122266 (+) 390 WP_045534311.1 hypothetical protein -
  SMCB_RS00580 (SMCB_0118) rsmI 122343..123293 (-) 951 WP_045534313.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  SMCB_RS00585 (SMCB_0119) - 123292..123783 (+) 492 WP_045534314.1 YraN family protein -
  SMCB_RS00590 (SMCB_0120) - 123894..124565 (+) 672 WP_045534315.1 BON domain-containing protein -
  SMCB_RS00595 (SMCB_0121) - 124645..125547 (-) 903 WP_045534318.1 NAD(P)-dependent oxidoreductase -
  SMCB_RS00600 (SMCB_0122) pilU 125554..126690 (-) 1137 WP_045534320.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SMCB_RS00605 (SMCB_0123) pilT 126739..127782 (-) 1044 WP_045534322.1 type IV pilus twitching motility protein PilT Machinery gene
  SMCB_RS00610 (SMCB_0124) - 127816..128520 (+) 705 WP_045534324.1 YggS family pyridoxal phosphate-dependent enzyme -
  SMCB_RS00615 (SMCB_0125) - 128517..129722 (-) 1206 WP_052468347.1 PAS domain-containing sensor histidine kinase -
  SMCB_RS00620 (SMCB_0126) - 129770..131275 (-) 1506 WP_045534325.1 FAD-linked oxidase C-terminal domain-containing protein -
  SMCB_RS00630 (SMCB_0127) - 131630..132514 (-) 885 WP_045534326.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38444.26 Da        Isoelectric Point: 7.1597

>NTDB_id=65936 SMCB_RS00605 WP_045534322.1 126739..127782(-) (pilT) [Serpentinimonas maccroryi strain B1]
MDITQLLAFSHKNKASDLHLSSGLPPMIRVHGDVRRLNVEPLEHKQVHAMVYDIMNDAQRKHYEEFLEVDFSFEIEGLAR
FRVNAFNQKRGAAAVFRTIPSKILSLEDLNCPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDYGHILTIED
PIEFVHESKKSLINQREVSTMTLSFAAALRSALREDPDVVLVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPPEEKEMVRAMLSESLVAVISQTLLKTADGNGRVAAHEIMMGTPAIRNLIRENKVAQMYSAIQTGNGFGMQTLDQS
LMNLVRGNRVHVAEARAKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=65936 SMCB_RS00605 WP_045534322.1 126739..127782(-) (pilT) [Serpentinimonas maccroryi strain B1]
ATGGACATCACCCAGCTGCTGGCCTTCAGCCACAAAAACAAAGCCTCAGACTTGCACCTGTCGTCTGGCCTGCCGCCAAT
GATACGGGTGCACGGCGACGTGCGCCGCCTCAACGTCGAGCCGCTCGAGCACAAGCAGGTGCACGCCATGGTCTATGACA
TCATGAACGACGCCCAGCGCAAGCACTACGAGGAGTTCCTCGAGGTCGATTTCTCATTCGAAATCGAGGGCTTGGCGCGC
TTTCGTGTCAACGCCTTCAACCAAAAGCGCGGCGCGGCGGCGGTGTTTCGCACCATTCCATCCAAAATCCTCTCGCTCGA
AGACCTCAACTGCCCCAAAATTTTTGCCGACCTCGCGCTCAAGCCGCGCGGCTTGGTGCTGGTCACGGGCCCGACCGGTT
CCGGCAAATCGACCACCCTGGCGGCGATGGTCAACCACCGCAACGAAAACGACTACGGCCACATCCTGACGATCGAAGAC
CCGATCGAGTTCGTGCACGAATCCAAAAAAAGCCTGATCAACCAGCGCGAAGTCAGTACCATGACGCTGAGCTTTGCGGC
GGCGCTGCGCTCGGCCCTGCGCGAAGACCCGGACGTGGTGCTGGTGGGCGAGATGCGCGACCTTGAAACCATCCGCTTGG
CCATGACCGCCGCCGAAACCGGGCACTTGGTGTTTGGCACCCTGCACACCTCGAGCGCGGCCAAGACCATCGACCGCATC
ATCGACGTCTTTCCGCCCGAAGAAAAAGAGATGGTGCGCGCCATGCTGTCGGAGTCGCTGGTGGCCGTGATCTCGCAGAC
CCTGCTCAAGACCGCCGACGGCAACGGCCGCGTGGCCGCGCACGAGATCATGATGGGCACGCCGGCGATCCGCAACCTGA
TCCGCGAAAACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCAACGGCTTTGGCATGCAGACGCTGGATCAGAGC
CTGATGAACCTGGTGCGTGGCAACCGCGTGCACGTGGCAGAGGCCCGTGCCAAAGCCAAAATCCCGGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.072

99.424

0.746
  pilT Acinetobacter nosocomialis M2

74.783

99.424

0.744
  pilT Acinetobacter baumannii D1279779

74.783

99.424

0.744
  pilT Acinetobacter baumannii strain A118

74.783

99.424

0.744
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

74.39

94.524

0.703
  pilT Legionella pneumophila strain ERS1305867

74.39

94.524

0.703
  pilT Neisseria meningitidis 8013

69.565

99.424

0.692
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.569

98.271

0.654
  pilT Vibrio cholerae strain A1552

66.569

98.271

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Acinetobacter baylyi ADP1

40.173

99.712

0.401
  pilU Vibrio cholerae strain A1552

41.194

96.542

0.398
  pilU Pseudomonas stutzeri DSM 10701

42.284

93.372

0.395

Multiple sequence alignment