Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SMCB_RS00600 Genome accession   NZ_AP014569
Coordinates   125554..126690 (-) Length   378 a.a.
NCBI ID   WP_045534320.1    Uniprot ID   A0A060NLX3
Organism   Serpentinimonas maccroryi strain B1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 120554..131690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMCB_RS00570 (SMCB_0116) - 121115..121735 (-) 621 WP_045534309.1 DUF502 domain-containing protein -
  SMCB_RS00575 (SMCB_0117) - 121877..122266 (+) 390 WP_045534311.1 hypothetical protein -
  SMCB_RS00580 (SMCB_0118) rsmI 122343..123293 (-) 951 WP_045534313.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  SMCB_RS00585 (SMCB_0119) - 123292..123783 (+) 492 WP_045534314.1 YraN family protein -
  SMCB_RS00590 (SMCB_0120) - 123894..124565 (+) 672 WP_045534315.1 BON domain-containing protein -
  SMCB_RS00595 (SMCB_0121) - 124645..125547 (-) 903 WP_045534318.1 NAD(P)-dependent oxidoreductase -
  SMCB_RS00600 (SMCB_0122) pilU 125554..126690 (-) 1137 WP_045534320.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SMCB_RS00605 (SMCB_0123) pilT 126739..127782 (-) 1044 WP_045534322.1 type IV pilus twitching motility protein PilT Machinery gene
  SMCB_RS00610 (SMCB_0124) - 127816..128520 (+) 705 WP_045534324.1 YggS family pyridoxal phosphate-dependent enzyme -
  SMCB_RS00615 (SMCB_0125) - 128517..129722 (-) 1206 WP_052468347.1 PAS domain-containing sensor histidine kinase -
  SMCB_RS00620 (SMCB_0126) - 129770..131275 (-) 1506 WP_045534325.1 FAD-linked oxidase C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41860.13 Da        Isoelectric Point: 7.3070

>NTDB_id=65935 SMCB_RS00600 WP_045534320.1 125554..126690(-) (pilU) [Serpentinimonas maccroryi strain B1]
MERDQASQFVNDLLRLLVSRNGSDLFLTADFPPAIKVDGKIAKVSPQPLSGQHTQALARALMNDKQSAEFERTMECNFAI
SPGGIGRFRVNAFVQMGKVGLVMRTIPAKIPTIDSLGLPQVLKNLALAKRGICILVGGTGSGKSTTLAAMVDWRNEHTHE
HIVTIEDPVEFVHQHKNCIVTQREVGLDTDGWDVALKNSLRQAPNVILMGEIRDRETMQHAISFSETGHLCLATLHANSA
NQAMERIINFFPEAVHAQILKDVSLNLRGMVAQRLLPRQDGKGRYAVVEILLHSGLIADQIMKGEINELKETMKKSVEVG
MQTFDQSLFTAFEANLISYEEALRNADSQNDLKLRIKLQSQRARNTDLGAGTEHLQIL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=65935 SMCB_RS00600 WP_045534320.1 125554..126690(-) (pilU) [Serpentinimonas maccroryi strain B1]
ATGGAACGCGATCAAGCCAGCCAATTCGTCAACGACCTGCTGCGCCTGCTGGTGAGCCGCAACGGCAGCGATTTGTTTCT
CACCGCCGACTTCCCGCCGGCGATCAAGGTCGACGGCAAGATCGCCAAGGTCTCGCCGCAGCCGCTTTCGGGCCAGCACA
CGCAGGCGCTGGCGCGCGCGCTCATGAACGACAAGCAGTCGGCCGAGTTCGAACGCACCATGGAGTGCAACTTTGCCATT
TCCCCCGGAGGTATTGGGCGCTTTCGCGTCAACGCCTTTGTGCAGATGGGTAAGGTCGGTCTGGTGATGCGCACCATTCC
GGCCAAAATCCCGACCATCGACAGCCTGGGGCTGCCGCAGGTGCTCAAAAACCTGGCGCTGGCCAAGCGCGGCATCTGCA
TCTTGGTGGGCGGCACCGGCTCGGGCAAATCGACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGCACACGCACGAG
CACATCGTGACCATCGAAGACCCGGTGGAGTTCGTGCACCAGCACAAAAACTGCATCGTGACGCAGCGCGAAGTGGGGCT
CGACACCGACGGCTGGGACGTTGCCCTCAAAAACTCGCTGCGCCAGGCGCCGAACGTGATTTTGATGGGCGAGATCCGGG
ACCGCGAAACCATGCAGCACGCGATCTCGTTCTCGGAAACCGGGCACTTGTGCTTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCCATGGAGCGCATCATCAACTTCTTCCCCGAAGCGGTGCACGCCCAGATTCTGAAGGACGTCTCGCTCAACCT
GCGCGGCATGGTGGCGCAGCGCCTGCTGCCGCGCCAAGACGGCAAGGGCCGCTACGCCGTGGTCGAGATTTTGCTGCACA
GCGGCCTGATCGCCGACCAGATCATGAAGGGCGAGATCAACGAGCTCAAGGAGACCATGAAAAAGAGCGTCGAGGTCGGG
ATGCAGACCTTCGACCAGTCGCTCTTTACGGCCTTCGAGGCCAACCTGATCAGCTACGAAGAAGCCCTGCGCAACGCCGA
CTCGCAAAACGACCTCAAGCTGCGCATCAAGCTGCAAAGCCAGCGCGCCCGCAACACCGATCTGGGGGCGGGCACCGAAC
ACCTGCAAATCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A060NLX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.312

92.328

0.548
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilU Acinetobacter baylyi ADP1

52.235

94.709

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

89.153

0.394
  pilT Legionella pneumophila strain ERS1305867

41.742

88.095

0.368
  pilT Legionella pneumophila strain Lp02

41.742

88.095

0.368
  pilT Acinetobacter nosocomialis M2

40.588

89.947

0.365
  pilT Acinetobacter baumannii D1279779

40.588

89.947

0.365
  pilT Acinetobacter baumannii strain A118

40.588

89.947

0.365
  pilT Pseudomonas aeruginosa PAK

40.653

89.153

0.362

Multiple sequence alignment