Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKD32_RS06215 Genome accession   NZ_CP092630
Coordinates   1394139..1395176 (+) Length   345 a.a.
NCBI ID   WP_037420775.1    Uniprot ID   A0A501XE78
Organism   Shewanella xiamenensis strain CQ-Y1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1389139..1400176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKD32_RS06185 (MKD32_06185) rdgB 1390195..1390812 (-) 618 WP_144375268.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  MKD32_RS06190 (MKD32_06190) - 1390927..1391361 (-) 435 WP_037420790.1 DUF4426 domain-containing protein -
  MKD32_RS06195 (MKD32_06195) yggU 1391435..1391725 (-) 291 WP_144375264.1 DUF167 family protein YggU -
  MKD32_RS06200 (MKD32_06200) - 1391725..1392273 (-) 549 WP_037420784.1 YggT family protein -
  MKD32_RS06205 (MKD32_06205) proC 1392348..1393166 (-) 819 WP_144344750.1 pyrroline-5-carboxylate reductase -
  MKD32_RS06210 (MKD32_06210) - 1393390..1394103 (-) 714 WP_203433159.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKD32_RS06215 (MKD32_06215) pilT 1394139..1395176 (+) 1038 WP_037420775.1 type IV pilus twitching motility protein PilT Machinery gene
  MKD32_RS06220 (MKD32_06220) pilU 1395186..1396298 (+) 1113 WP_037420771.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKD32_RS06225 (MKD32_06225) - 1396470..1397003 (+) 534 WP_037420768.1 glutathione peroxidase -
  MKD32_RS06230 (MKD32_06230) hemH 1397072..1398055 (+) 984 WP_240293187.1 ferrochelatase -
  MKD32_RS06235 (MKD32_06235) ruvX 1398119..1398544 (-) 426 WP_037420762.1 Holliday junction resolvase RuvX -
  MKD32_RS06240 (MKD32_06240) - 1398574..1399137 (-) 564 WP_037420759.1 YqgE/AlgH family protein -
  MKD32_RS06245 (MKD32_06245) yciH 1399206..1399535 (-) 330 WP_047533731.1 stress response translation initiation inhibitor YciH -
  MKD32_RS06250 (MKD32_06250) - 1399698..1400012 (-) 315 WP_037420754.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38278.98 Da        Isoelectric Point: 6.9096

>NTDB_id=659299 MKD32_RS06215 WP_037420775.1 1394139..1395176(+) (pilT) [Shewanella xiamenensis strain CQ-Y1]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHPNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=659299 MKD32_RS06215 WP_037420775.1 1394139..1395176(+) (pilT) [Shewanella xiamenensis strain CQ-Y1]
ATGGAAATCACTGAGTTATTAGCTTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATCAATCTGCCCGCGCTCGATCATCAGGGTGTACACAGCCTTGTTTATGACA
TTATGAATGATAAACAACGCAAGGACTTTGAAGAACATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTGAACGCCTTTAACCAATCTCGCGGCGCTGCTGCGGTATTTCGTACCATTCCCAGTGATATCTTGTCACTTGA
GCAGCTAGGTGCGCCTGAAATTTTTAAAAAAATTGCCAGTTTTCCCCGTGGGTTAGTGTTAGTCACTGGGCCGACAGGTT
CCGGTAAGAGTACCACACTTGCGGCCATGGTCGATTACATCAATGAAAACCGCCATGATCATATCTTAACCATTGAAGAC
CCAATCGAATTTGTTCATCCCAACAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGTCATACCCACAGCTTTAACGC
GGCACTGCGTAGCGCATTGCGTGAAGACCCTGACGTTATCCTCGTGGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CGATGACGGCGGCTGAAACGGGTCACTTAGTTTTTGGCACCTTACACACCACGTCGGCGGCTAAGACTATTGACCGTGTG
GTTGACGTTTTCCCTGCCGGTGAAAAGGACATGGTTCGTACCATGTTATCTGAATCATTACAGGCGGTTATTTCACAAAC
CCTGATTAAGAAAATCGGTGGTGGCCGTGTTGCTGCCCACGAAATTATGATGGGTACGCCCGCTATCCGAAATCTTATCC
GTGAAGATAAAGTGGCGCAGATGTACTCTGCCATTCAAACGGGGATGGCCCATGGCATGCAAACACTCGAACAGTGTCTG
CAAAACTTAGTTAACCGTGGCCTCATCACCCGTGAGGATGCCATGGCGAAGAGCTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A501XE78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Acinetobacter nosocomialis M2

80.117

99.13

0.794
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.691

99.42

0.414
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391