Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MKD32_RS06220 Genome accession   NZ_CP092630
Coordinates   1395186..1396298 (+) Length   370 a.a.
NCBI ID   WP_037420771.1    Uniprot ID   -
Organism   Shewanella xiamenensis strain CQ-Y1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1390186..1401298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKD32_RS06185 (MKD32_06185) rdgB 1390195..1390812 (-) 618 WP_144375268.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  MKD32_RS06190 (MKD32_06190) - 1390927..1391361 (-) 435 WP_037420790.1 DUF4426 domain-containing protein -
  MKD32_RS06195 (MKD32_06195) yggU 1391435..1391725 (-) 291 WP_144375264.1 DUF167 family protein YggU -
  MKD32_RS06200 (MKD32_06200) - 1391725..1392273 (-) 549 WP_037420784.1 YggT family protein -
  MKD32_RS06205 (MKD32_06205) proC 1392348..1393166 (-) 819 WP_144344750.1 pyrroline-5-carboxylate reductase -
  MKD32_RS06210 (MKD32_06210) - 1393390..1394103 (-) 714 WP_203433159.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKD32_RS06215 (MKD32_06215) pilT 1394139..1395176 (+) 1038 WP_037420775.1 type IV pilus twitching motility protein PilT Machinery gene
  MKD32_RS06220 (MKD32_06220) pilU 1395186..1396298 (+) 1113 WP_037420771.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKD32_RS06225 (MKD32_06225) - 1396470..1397003 (+) 534 WP_037420768.1 glutathione peroxidase -
  MKD32_RS06230 (MKD32_06230) hemH 1397072..1398055 (+) 984 WP_240293187.1 ferrochelatase -
  MKD32_RS06235 (MKD32_06235) ruvX 1398119..1398544 (-) 426 WP_037420762.1 Holliday junction resolvase RuvX -
  MKD32_RS06240 (MKD32_06240) - 1398574..1399137 (-) 564 WP_037420759.1 YqgE/AlgH family protein -
  MKD32_RS06245 (MKD32_06245) yciH 1399206..1399535 (-) 330 WP_047533731.1 stress response translation initiation inhibitor YciH -
  MKD32_RS06250 (MKD32_06250) - 1399698..1400012 (-) 315 WP_037420754.1 hypothetical protein -
  MKD32_RS06255 (MKD32_06255) - 1400191..1400751 (+) 561 WP_037420751.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41117.37 Da        Isoelectric Point: 6.5054

>NTDB_id=659300 MKD32_RS06220 WP_037420771.1 1395186..1396298(+) (pilU) [Shewanella xiamenensis strain CQ-Y1]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESVMTEAQKKEFHTTRECNFAFAVKELGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVAKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKEPASSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=659300 MKD32_RS06220 WP_037420771.1 1395186..1396298(+) (pilU) [Shewanella xiamenensis strain CQ-Y1]
ATGGATGTCCGTCCGTTTTTAAAAGTCATGGTGGAGCGTAAAGCCTCGGACCTGTTTATTACCGCAGGGTTTCCGCCGAG
CGCCAAAATCGATGGTGAGTTACGCCCGTTAGCCGAGAGTGCCTTCACGCCCGCGCAGTCTTTGGATTTTGTCGAGTCCG
TGATGACGGAGGCGCAAAAGAAGGAGTTTCACACGACTCGCGAGTGTAACTTTGCTTTTGCGGTTAAGGAGTTGGGGCGT
TTTCGCGTCAGCGCATTCTGGCAGCGTGAGTCTCCGGGTTGTGTTATGCGCCGGATTGAGACGAAAATCCCAGAAGTGGA
TGATTTAAAACTGCCACCCATTTTAAAAGATCTGGTGATGAGTAAGCGTGGACTTATCATCATGGTGGGGGGGACAGGGA
CCGGTAAATCGACTTCCTTAGCAGCATTAGTCGGTTACCGCAATGCCCATGCCCGCGGGCATATCTTGACGATTGAAGAC
CCTGTGGAATTTGTGCACGATCATCGCAAAAGTATCATTACCCAACGTGAAGTGGGCATAGACACTGAGTCCTTTGATGC
GGCGCTGAAGAGCTCGCTGCGTCAGGCGCCTGATGTGATCCTTATCGGTGAAATTCGAACCCAAGAAACCATGGAATTTG
CGCTCTCCTTTGCTGAAACGGGCCATCTGTGTATGGCGACACTGCATGCTAACAATGCTAACCAAGCATTAGACCGGATT
ATGCACTTAGTGCCAGAGAGTAAACATCAACAGCTATTATTCGATTTGTCACTCAACCTGCGTGGCATTGTGGCGCAGCA
GCTGGTGGCTAAAGTTGATGGAACAGGGCGCCGAGCGGCGATTGAAGTCTTGATCAATACGCCACGTGTCGCGAGTTTGA
TTGCTAAAAACGAGCTGCATTTGCTCAAAGAAACCATGGCCAAATCGAATGAGCAGGGCATGCAGACCTTTGACCAAGCT
CTGTTAAAGCTCTATGTCGATGGGGAGATCAGTTATGCCGACGCGCTTCACCATGCTGACTCACCCAACGACTTGCGGTT
AATGATTAAATTGCAAAATAAAGAACCCGCCAGTTCGAGCTTTATGGAAGGCGTGACCTTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.108

100

0.603
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.988

88.649

0.381
  pilT Vibrio cholerae strain A1552

42.988

88.649

0.381
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.29

93.243

0.376
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Legionella pneumophila strain Lp02

40.785

89.459

0.365
  pilT Legionella pneumophila strain ERS1305867

40.785

89.459

0.365