Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ATS73_RS12100 Genome accession   NZ_CP092598
Coordinates   2541900..2542940 (-) Length   346 a.a.
NCBI ID   WP_058433150.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. H100     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2536900..2547940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATS73_RS12070 (ATS73_011990) - 2537070..2537546 (+) 477 WP_006793185.1 hypothetical protein -
  ATS73_RS12075 (ATS73_011995) rsmE 2537621..2538355 (+) 735 Protein_2309 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ATS73_RS12080 (ATS73_012000) gshB 2538432..2539382 (+) 951 WP_008132396.1 glutathione synthase -
  ATS73_RS12085 (ATS73_012005) - 2539551..2540108 (+) 558 WP_008132397.1 YqgE/AlgH family protein -
  ATS73_RS12090 (ATS73_012010) ruvX 2540237..2540686 (+) 450 WP_058429707.1 Holliday junction resolvase RuvX -
  ATS73_RS12095 (ATS73_012015) pilU 2540760..2541890 (-) 1131 WP_006793190.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ATS73_RS12100 (ATS73_012020) pilT 2541900..2542940 (-) 1041 WP_058433150.1 type IV pilus twitching motility protein PilT Machinery gene
  ATS73_RS12105 (ATS73_012025) - 2542943..2543656 (+) 714 WP_058433149.1 YggS family pyridoxal phosphate-dependent enzyme -
  ATS73_RS12110 (ATS73_012030) proC 2543674..2544495 (+) 822 WP_058433148.1 pyrroline-5-carboxylate reductase -
  ATS73_RS12115 (ATS73_012035) - 2544522..2545056 (+) 535 Protein_2317 YggT family protein -
  ATS73_RS12120 (ATS73_012040) - 2545117..2545563 (+) 447 WP_058433147.1 DUF4426 domain-containing protein -
  ATS73_RS12125 (ATS73_012045) - 2545568..2546167 (+) 600 WP_008132404.1 XTP/dITP diphosphatase -
  ATS73_RS12130 (ATS73_012050) hemW 2546164..2547300 (+) 1137 WP_058433146.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38080.33 Da        Isoelectric Point: 6.1262

>NTDB_id=659233 ATS73_RS12100 WP_058433150.1 2541900..2542940(-) (pilT) [Pseudoalteromonas sp. H100]
MDITELLAFSVQHNASDLHLSSGVSPMIRVDGDVRRINIPALTDKDVNSLVYDIMNDTQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLDDLGAPDIFKTISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQNKHHHILTIED
PIEFVHDNKLSLVNQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKAMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIGIPAIRNLIREDKIAQMYSSIQTGASIGMQTMDQCL
TNLVNRGIVTNTAAQAKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=659233 ATS73_RS12100 WP_058433150.1 2541900..2542940(-) (pilT) [Pseudoalteromonas sp. H100]
ATGGATATTACCGAATTATTAGCGTTTAGTGTGCAACACAATGCATCCGATTTACATTTATCGTCGGGTGTTTCACCTAT
GATACGCGTAGATGGTGATGTTCGCCGCATTAATATACCAGCCCTTACAGATAAAGACGTAAACAGTTTAGTTTACGATA
TTATGAACGATACTCAGCGCAAAGATTACGAACAAAACCTTGAGGTGGATTTTTCGTTTGAGGTTCCTAATCTTGCTCGC
TTTCGTGTTAATGCCTTTAATTCAAACCGCGGTCCTGCGGCAGTATTTCGTACTATACCGAGTGAAGTATTAACACTTGA
TGATTTAGGCGCGCCAGATATTTTTAAAACAATATCAGATAACCCGCGTGGGTTAGTGCTTGTTACCGGGCCAACAGGTT
CGGGTAAATCAACAACACTTGCGGCCATGGTTGATTACATAAATCAAAATAAGCATCACCATATTTTAACGATTGAAGAT
CCAATCGAATTTGTTCACGATAACAAGTTAAGCTTAGTTAACCAACGTGAAGTGCATCGTGATACACACAGTTTTTCAAA
CGCACTACGTAGTGCGCTGCGTGAAGACCCCGATGTTATATTAGTGGGTGAGTTGCGTGATTTAGAAACTATTCGCCTAG
CGATGACCGCTGCTGAAACCGGTCATTTAGTGTTTGGTACGTTGCATACAACCTCAGCGCCCAAAACCATTGACCGTATT
ATTGATGTATTCCCTGGTGAAGAAAAAGCAATGGTACGCTCTATGTTGTCTGAGTCATTGCGTGCGGTTATTTCACAAAC
GCTCCTTAAAAAAATTGGTGGTGGGCGAGTGGCAGCTCATGAAATTATGATTGGTATACCCGCCATACGTAACTTAATTC
GTGAAGACAAAATTGCACAAATGTACTCGTCTATTCAAACTGGCGCCTCAATTGGTATGCAAACAATGGATCAATGTTTA
ACAAACTTAGTTAACCGAGGCATTGTGACTAACACCGCAGCGCAAGCTAAAGCGCAAGACAAAACACAATTTGGTGGTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.744

99.422

0.763
  pilT Acinetobacter baumannii strain A118

76.744

99.422

0.763
  pilT Acinetobacter nosocomialis M2

76.744

99.422

0.763
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.422

0.76
  pilT Pseudomonas aeruginosa PAK

76.163

99.422

0.757
  pilT Acinetobacter baylyi ADP1

75.872

99.422

0.754
  pilT Legionella pneumophila strain Lp02

72.384

99.422

0.72
  pilT Legionella pneumophila strain ERS1305867

72.384

99.422

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.977

0.699
  pilT Vibrio cholerae strain A1552

71.386

97.977

0.699
  pilT Neisseria gonorrhoeae MS11

69.501

98.555

0.685
  pilT Neisseria meningitidis 8013

69.501

98.555

0.685
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.711

0.514
  pilU Vibrio cholerae strain A1552

42.154

93.931

0.396
  pilU Pseudomonas stutzeri DSM 10701

40.181

95.665

0.384
  pilU Acinetobacter baylyi ADP1

38.838

94.509

0.367