Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ATS73_RS12095 Genome accession   NZ_CP092598
Coordinates   2540760..2541890 (-) Length   376 a.a.
NCBI ID   WP_006793190.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. H100     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2535760..2546890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATS73_RS12065 (ATS73_011985) - 2536103..2536996 (+) 894 Protein_2307 hypothetical protein -
  ATS73_RS12070 (ATS73_011990) - 2537070..2537546 (+) 477 WP_006793185.1 hypothetical protein -
  ATS73_RS12075 (ATS73_011995) rsmE 2537621..2538355 (+) 735 Protein_2309 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ATS73_RS12080 (ATS73_012000) gshB 2538432..2539382 (+) 951 WP_008132396.1 glutathione synthase -
  ATS73_RS12085 (ATS73_012005) - 2539551..2540108 (+) 558 WP_008132397.1 YqgE/AlgH family protein -
  ATS73_RS12090 (ATS73_012010) ruvX 2540237..2540686 (+) 450 WP_058429707.1 Holliday junction resolvase RuvX -
  ATS73_RS12095 (ATS73_012015) pilU 2540760..2541890 (-) 1131 WP_006793190.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ATS73_RS12100 (ATS73_012020) pilT 2541900..2542940 (-) 1041 WP_058433150.1 type IV pilus twitching motility protein PilT Machinery gene
  ATS73_RS12105 (ATS73_012025) - 2542943..2543656 (+) 714 WP_058433149.1 YggS family pyridoxal phosphate-dependent enzyme -
  ATS73_RS12110 (ATS73_012030) proC 2543674..2544495 (+) 822 WP_058433148.1 pyrroline-5-carboxylate reductase -
  ATS73_RS12115 (ATS73_012035) - 2544522..2545056 (+) 535 Protein_2317 YggT family protein -
  ATS73_RS12120 (ATS73_012040) - 2545117..2545563 (+) 447 WP_058433147.1 DUF4426 domain-containing protein -
  ATS73_RS12125 (ATS73_012045) - 2545568..2546167 (+) 600 WP_008132404.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41688.53 Da        Isoelectric Point: 5.6307

>NTDB_id=659232 ATS73_RS12095 WP_006793190.1 2540760..2541890(-) (pilU) [Pseudoalteromonas sp. H100]
MTLSLNRYLITMIEKKGSDLFVSSRLPVSAKINGELIQLGDDKLTDEESLQLVESAMSEKQKAEFHATKECNFAIATDEG
RFRISAFWQRDRAGMVIRRIVTQIPDVSDLGLPSTLTDVIMAKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLIPTSDGDGRVAAIEILLNSPMVAELIKNGDIGGIKEAMAKSNDMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGMGDDDN

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=659232 ATS73_RS12095 WP_006793190.1 2540760..2541890(-) (pilU) [Pseudoalteromonas sp. H100]
ATGACTTTATCTTTAAACCGTTACTTAATCACTATGATTGAGAAAAAAGGCTCTGATTTATTTGTATCTAGCCGTTTACC
TGTTAGTGCAAAAATAAACGGTGAACTAATTCAATTGGGCGATGACAAACTCACTGACGAAGAGTCTCTGCAGTTGGTTG
AGTCTGCGATGAGCGAAAAGCAAAAAGCGGAGTTTCATGCTACTAAAGAATGTAACTTTGCTATTGCAACCGACGAAGGG
CGTTTTCGTATTTCTGCGTTTTGGCAGCGCGATAGAGCAGGTATGGTTATTCGCCGAATTGTGACACAGATCCCCGATGT
CAGTGATTTGGGCCTACCCTCGACACTCACCGATGTCATTATGGCTAAGCGCGGTTTAGTTTTGTTTGTTGGTGGAACAG
GAACCGGTAAGTCGACCTCATTAGCAGCCTTAATTGGCTACAGAAACCGAAATCAACGGGGTCATATTTTAACAATTGAA
GATCCTATTGAGTTTGTTCATGAGCACCGCAAAAGTATTATTACCCAACGCGAAGTAGGGCTCGATACAGATAGTTTTGA
GTCGGCGCTTAAAAGCTCATTACGTCAAGCTCCTGATGTTATTTTAATTGGTGAAATACGCTCGCAAGAAACCATGGAAT
ACGCATTAAGCTTTGCTGAAACAGGGCATTTATGTGTGGCTACACTGCATGCCAACAATGCCAACCAAGCCATTGACCGT
ATTATGCATTTGGTACCAAAAGAAAAGCATGACAAGCTAAAATACGATCTAGCCCTTAACTTACGCGCCATTGTAGCTCA
ACAATTAATACCTACCTCAGATGGTGACGGCCGCGTTGCCGCCATAGAAATATTGCTTAATTCACCTATGGTGGCAGAGC
TGATTAAAAACGGCGATATTGGCGGCATTAAAGAGGCCATGGCTAAATCTAACGATATGGGCATGCAAACCTTTGACCAA
GCCCTTTTTGAGTTATACAGGCAGCAACGTATTAATTATGCAGATGCACTGCATCATGCTGACTCACCAAATGACTTACG
CTTGATGATTAAACTGCGCAATAACGAACAACAAGGTGCGGGCTTTTTGCAAGGTGTCACTATTGATGGAATGGGGGATG
ACGATAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.56

100

0.577
  pilU Acinetobacter baylyi ADP1

55.968

100

0.561
  pilU Vibrio cholerae strain A1552

55.858

97.606

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.84

92.819

0.407