Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NEJAP_RS01440 Genome accession   NZ_AP014546
Coordinates   315489..316523 (+) Length   344 a.a.
NCBI ID   WP_201348962.1    Uniprot ID   A0A7R6PEV0
Organism   Neptunomonas japonica JAMM 1380     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 310489..321523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEJAP_RS01405 (NEJAP_0273) - 310513..310947 (-) 435 WP_201348955.1 DUF4426 domain-containing protein -
  NEJAP_RS01410 (NEJAP_0274) metW 310960..311550 (-) 591 WP_201348956.1 methionine biosynthesis protein MetW -
  NEJAP_RS01415 (NEJAP_0275) metX 311540..312736 (-) 1197 WP_201348957.1 homoserine O-succinyltransferase MetX -
  NEJAP_RS01420 (NEJAP_0276) - 312843..313133 (-) 291 WP_201348958.1 DUF167 family protein -
  NEJAP_RS01425 (NEJAP_0277) - 313146..313694 (-) 549 WP_201348959.1 YggT family protein -
  NEJAP_RS01430 (NEJAP_0278) proC 313849..314676 (-) 828 WP_201348960.1 pyrroline-5-carboxylate reductase -
  NEJAP_RS01435 (NEJAP_0279) - 314695..315402 (-) 708 WP_201348961.1 YggS family pyridoxal phosphate-dependent enzyme -
  NEJAP_RS01440 (NEJAP_0280) pilT 315489..316523 (+) 1035 WP_201348962.1 type IV pilus twitching motility protein PilT Machinery gene
  NEJAP_RS01445 (NEJAP_0281) pilU 316579..317745 (+) 1167 WP_201348963.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NEJAP_RS01450 (NEJAP_0282) - 317808..319079 (-) 1272 WP_201348964.1 dihydroorotase -
  NEJAP_RS01455 (NEJAP_0283) - 319085..320083 (-) 999 WP_201348965.1 aspartate carbamoyltransferase catalytic subunit -
  NEJAP_RS01460 (NEJAP_0284) pyrR 320102..320611 (-) 510 WP_201348966.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NEJAP_RS01465 (NEJAP_0285) ruvX 320586..321068 (-) 483 WP_201348967.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38064.73 Da        Isoelectric Point: 6.7063

>NTDB_id=65873 NEJAP_RS01440 WP_201348962.1 315489..316523(+) (pilT) [Neptunomonas japonica JAMM 1380]
MDITELLSFTVQQGASDLHITAGMPPVIRVDGDVRRIKLPALEQKQVQTLIYDIMNDKIRKDFEDRFEADFSFEVPGLAR
FRVNAFNQNRGAAAVFRTIPSKVLTMDELGMGKVFQDLSMQARGLVLVTGPTGSGKSTTLAAMVDYVNEVRSDHILTIED
PIEFVHESKKCLVNQREVHRDTQSFANALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMVRSMLSESLQGVISQTLLKKVNGGRVAAHEIMMGTPAVRNLIREDKVAQMYSAIQTGASHGMKTLDQSL
TELVQKGLISRETARDKAKNPQGF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=65873 NEJAP_RS01440 WP_201348962.1 315489..316523(+) (pilT) [Neptunomonas japonica JAMM 1380]
ATGGATATTACAGAACTTCTTTCGTTTACAGTTCAGCAGGGCGCTTCCGACCTGCATATAACGGCAGGCATGCCGCCAGT
TATTCGTGTTGATGGAGATGTACGGCGCATTAAGCTGCCAGCGTTAGAGCAGAAGCAAGTTCAGACGCTTATTTATGACA
TCATGAACGATAAAATCCGTAAAGATTTTGAAGATAGGTTTGAAGCGGATTTTTCTTTTGAAGTCCCTGGGTTAGCACGT
TTTCGTGTCAATGCTTTTAATCAGAACCGCGGAGCTGCAGCTGTTTTTCGTACTATTCCAAGTAAAGTTCTGACAATGGA
CGAGCTGGGAATGGGAAAAGTTTTCCAAGACTTATCAATGCAGGCACGAGGTTTAGTACTCGTAACAGGACCTACCGGTT
CAGGTAAGAGTACAACATTGGCAGCGATGGTTGATTATGTTAATGAAGTGCGCTCCGATCATATCTTGACGATTGAAGAT
CCTATTGAGTTTGTACATGAAAGCAAAAAATGCTTAGTGAATCAGCGTGAAGTACACAGAGATACGCAGAGTTTTGCTAA
TGCATTACGTTCAGCGTTACGTGAGGATCCTGACATTATCCTTGTGGGTGAGATGCGAGATCTAGAAACCATACGTTTGG
CATTGACTGCTGCGGAAACGGGTCACCTTGTGTTTGGTACCTTGCATACAACATCTGCTGCGAAGACGATTGACCGTATT
ATCGATGTGTTCCCTGCGGCTGAAAAAGATATGGTGCGTTCAATGCTATCAGAGTCTTTGCAAGGTGTTATCTCCCAAAC
ATTGCTTAAAAAAGTTAATGGGGGGCGTGTTGCCGCGCATGAAATCATGATGGGAACTCCTGCTGTTCGAAACTTGATTC
GTGAAGATAAAGTCGCGCAGATGTACTCCGCGATTCAAACGGGTGCTTCGCATGGAATGAAAACATTAGATCAATCCCTG
ACAGAGCTTGTTCAAAAAGGCCTGATATCAAGAGAAACGGCTCGCGATAAAGCCAAAAACCCACAAGGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7R6PEV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.977

100

0.82
  pilT Pseudomonas stutzeri DSM 10701

80.233

100

0.802
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

72.674

100

0.727
  pilT Legionella pneumophila strain ERS1305867

72.674

100

0.727
  pilT Neisseria meningitidis 8013

64.928

100

0.651
  pilT Neisseria gonorrhoeae MS11

64.638

100

0.648
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.965

0.648
  pilT Vibrio cholerae strain A1552

66.172

97.965

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.5
  pilU Vibrio cholerae strain A1552

42.985

97.384

0.419
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387

Multiple sequence alignment