Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NEJAP_RS01445 Genome accession   NZ_AP014546
Coordinates   316579..317745 (+) Length   388 a.a.
NCBI ID   WP_201348963.1    Uniprot ID   A0A7R6P9C7
Organism   Neptunomonas japonica JAMM 1380     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 311579..322745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEJAP_RS01420 (NEJAP_0276) - 312843..313133 (-) 291 WP_201348958.1 DUF167 family protein -
  NEJAP_RS01425 (NEJAP_0277) - 313146..313694 (-) 549 WP_201348959.1 YggT family protein -
  NEJAP_RS01430 (NEJAP_0278) proC 313849..314676 (-) 828 WP_201348960.1 pyrroline-5-carboxylate reductase -
  NEJAP_RS01435 (NEJAP_0279) - 314695..315402 (-) 708 WP_201348961.1 YggS family pyridoxal phosphate-dependent enzyme -
  NEJAP_RS01440 (NEJAP_0280) pilT 315489..316523 (+) 1035 WP_201348962.1 type IV pilus twitching motility protein PilT Machinery gene
  NEJAP_RS01445 (NEJAP_0281) pilU 316579..317745 (+) 1167 WP_201348963.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NEJAP_RS01450 (NEJAP_0282) - 317808..319079 (-) 1272 WP_201348964.1 dihydroorotase -
  NEJAP_RS01455 (NEJAP_0283) - 319085..320083 (-) 999 WP_201348965.1 aspartate carbamoyltransferase catalytic subunit -
  NEJAP_RS01460 (NEJAP_0284) pyrR 320102..320611 (-) 510 WP_201348966.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NEJAP_RS01465 (NEJAP_0285) ruvX 320586..321068 (-) 483 WP_201348967.1 Holliday junction resolvase RuvX -
  NEJAP_RS01470 (NEJAP_0286) - 321101..321667 (-) 567 WP_201348968.1 YqgE/AlgH family protein -
  NEJAP_RS01475 (NEJAP_0287) - 321706..322668 (-) 963 WP_236591022.1 energy transducer TonB -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43497.86 Da        Isoelectric Point: 5.6217

>NTDB_id=65874 NEJAP_RS01445 WP_201348963.1 316579..317745(+) (pilU) [Neptunomonas japonica JAMM 1380]
MEFTQLLKVMIERGASDLFVSAGARPSIKVDGTLKPLTKDPLKPSQARSLVYSTMTDKQISEYESTHECNFAISAPGLGR
FRVSAYFQRNSPGMVLRRINATIPSFEELNLPPVMKDLSMSKRGLILFVGGTSTGKSTSLASMIDYRNRHSRGHIITIED
PIEYIHEHKESIVTQREVGIDTDSFEVALRNTLRQAPDVILMGEIRTQELMKYGLAFAETGHLCMATLHANNANQALDRV
VSFFPPEHHDQLWMELSLNLKAIVAQQLLPTKDGKGRRAAIEVMICTPLIQDLIRKGDIHEIKEVMKKSTNLGMQTFDQA
LFELYKEEAINYDTALAHADSANDLRLMIKLSADTNPGLSMQDDTDSLFLQEEEDFMNVSGEMEIGKM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=65874 NEJAP_RS01445 WP_201348963.1 316579..317745(+) (pilU) [Neptunomonas japonica JAMM 1380]
GTGGAGTTTACTCAGCTTCTTAAAGTAATGATTGAGCGCGGAGCGTCAGATCTGTTTGTCTCGGCAGGGGCTCGACCGAG
TATTAAGGTTGATGGCACGCTGAAGCCATTAACAAAGGATCCGTTGAAACCATCTCAGGCGCGCTCGTTAGTGTATTCGA
CGATGACGGATAAGCAAATATCCGAATATGAAAGTACACATGAATGTAATTTTGCTATTAGCGCGCCAGGTTTGGGGCGT
TTTCGTGTAAGTGCTTACTTTCAGCGTAACTCACCAGGCATGGTGCTTCGACGAATTAATGCGACTATTCCTAGCTTTGA
AGAGTTAAACCTTCCTCCCGTAATGAAAGACCTTTCCATGTCTAAGCGTGGTTTGATCTTATTTGTTGGTGGTACATCAA
CGGGTAAATCAACATCGCTGGCGTCGATGATCGACTATCGTAACCGGCATAGCCGTGGACACATCATCACGATAGAAGAC
CCGATTGAGTATATTCATGAGCATAAAGAGAGCATTGTGACTCAGCGTGAAGTGGGCATCGATACGGACTCATTTGAAGT
GGCGTTAAGGAATACCTTAAGACAAGCCCCTGATGTTATTCTGATGGGTGAGATTCGAACTCAAGAATTGATGAAATACG
GATTGGCGTTTGCAGAAACTGGGCATCTTTGTATGGCCACTTTACATGCAAATAATGCTAACCAGGCCCTTGATAGAGTT
GTAAGTTTTTTCCCGCCAGAACATCATGACCAGCTCTGGATGGAGTTGTCGCTTAACTTAAAAGCGATTGTTGCCCAGCA
ATTACTGCCTACAAAGGATGGTAAAGGGCGTCGTGCGGCAATAGAAGTAATGATTTGCACGCCATTAATTCAGGATTTAA
TCCGTAAAGGTGATATCCATGAAATTAAAGAGGTCATGAAAAAGTCGACTAACTTAGGGATGCAAACATTTGATCAGGCG
TTGTTTGAGCTTTATAAAGAAGAAGCTATTAATTATGACACTGCGTTAGCGCATGCTGACTCAGCGAATGATTTACGTCT
GATGATTAAGTTAAGTGCGGATACAAACCCTGGATTAAGTATGCAAGATGATACTGATTCGCTGTTTTTGCAGGAAGAAG
AAGACTTTATGAATGTGTCTGGTGAAATGGAAATTGGCAAAATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7R6P9C7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.292

91.753

0.608
  pilU Acinetobacter baylyi ADP1

61.389

92.784

0.57
  pilU Vibrio cholerae strain A1552

54.857

90.206

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.5

92.784

0.394
  pilT Acinetobacter baylyi ADP1

42.229

87.887

0.371
  pilT Pseudomonas aeruginosa PAK

42.985

86.34

0.371
  pilT Legionella pneumophila strain ERS1305867

42.388

86.34

0.366
  pilT Legionella pneumophila strain Lp02

42.388

86.34

0.366
  pilT Pseudomonas stutzeri DSM 10701

42.09

86.34

0.363
  pilT Acinetobacter nosocomialis M2

41.791

86.34

0.361
  pilT Acinetobacter baumannii D1279779

41.791

86.34

0.361
  pilT Acinetobacter baumannii strain A118

41.791

86.34

0.361

Multiple sequence alignment