Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MI302_RS11355 Genome accession   NZ_CP092445
Coordinates   1985975..1987063 (+) Length   362 a.a.
NCBI ID   WP_239725833.1    Uniprot ID   -
Organism   Thermus sp. NEB1569     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1980975..1992063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MI302_RS11330 (MI302_11330) pgeF 1981119..1981844 (+) 726 WP_019551474.1 peptidoglycan editing factor PgeF -
  MI302_RS11335 (MI302_11335) - 1981876..1982364 (+) 489 WP_126178010.1 YqeG family HAD IIIA-type phosphatase -
  MI302_RS11340 (MI302_11340) - 1982378..1982962 (+) 585 Protein_2123 type II/IV secretion system protein -
  MI302_RS11345 (MI302_11345) - 1983018..1983863 (+) 846 WP_038032087.1 transposase -
  MI302_RS11350 (MI302_11350) pilF 1983885..1985963 (+) 2079 WP_370687348.1 type II/IV secretion system protein Machinery gene
  MI302_RS11355 (MI302_11355) pilT 1985975..1987063 (+) 1089 WP_239725833.1 type IV pilus twitching motility protein PilT Machinery gene
  MI302_RS11360 (MI302_11360) gatB 1987087..1988496 (+) 1410 WP_239725834.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  MI302_RS11365 (MI302_11365) purM 1988502..1989503 (+) 1002 WP_126200626.1 phosphoribosylformylglycinamidine cyclo-ligase -
  MI302_RS11370 (MI302_11370) - 1989500..1990129 (+) 630 WP_126169942.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40176.50 Da        Isoelectric Point: 7.5848

>NTDB_id=657538 MI302_RS11355 WP_239725833.1 1985975..1987063(+) (pilT) [Thermus sp. NEB1569]
MAKTPDIVDLLTLAVERRASDLVITVGLPPMIKVDGEFHPTEYEPLSPQDTRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPATIKSFEELGLPKNIAEIALSPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHKHKKAIVNQREIGSDTHGFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMIT
MDACLADLYKRKLITYELGLSRAVDPKEFMRLSGVQEGAKRP

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=657538 MI302_RS11355 WP_239725833.1 1985975..1987063(+) (pilT) [Thermus sp. NEB1569]
ATGGCCAAAACACCCGACATCGTGGACCTCTTAACCCTGGCCGTGGAGCGGAGGGCCAGCGACCTGGTGATCACCGTGGG
CCTTCCCCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCTCCCCCCAGGACACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAACGGATCTTTGAGGAGGAAAAGGAGCTGGACTTCTCCTTCAGCCTCCCG
GGAAAGGGCCGCTACCGGGTCAACGTCTTCCTGCAACGGGGAAGCGTGGGCGGGGTCCTTAGGGTGGTGCCCGCCACCAT
CAAGAGCTTTGAGGAGCTGGGCCTGCCCAAGAACATCGCCGAGATCGCCTTAAGCCCCAGAGGCCTGGTCCTGGTCACGG
GGCCCACCGGGTCGGGGAAGAGCACCACCTTGGCCTCCATGATCGACTACATCAACGAGCGCAAGCCGGTGCACATCGTG
ACCATCGAGGACCCCATTGAGTTTTTCCACAAGCACAAGAAGGCCATCGTCAACCAGCGGGAGATCGGCTCCGATACCCA
CGGGTTCCACAAGGCCCTGAGAAGCGTTCTTCGCCAGGCCCCGGACGTGATCCTGGTGGGGGAGATGCGGGACTACGAAA
CCATCGCCGCCGCCATCACCGCTGCGGAAACCGGGCACCTGGTCATGGGCACCCTGCACACCAACTCCGCCCCCGAGACC
ATAGACCGCATCATCGACGTCTTCCCCGAAAACCAGCAGGAGCAGGTGCGGGTGCAGCTTTCCAACAACCTGGTGGCCGT
TTTAACCCAGCAGCTCCTGCCCAAGGCCTTTGGGGGCGGGCGGGTCTTGGCCTACGAGCTCATGATCGCTACCCCTGCGG
TAAGGGCCCTGATCCGGGAGGGGAAGAGCCACCAGCTTAGGAGCGTCATCCAGACCGGGGGCCAGTACGGGATGATCACC
ATGGACGCTTGCCTTGCTGACCTTTACAAGCGCAAGCTGATCACCTACGAGCTGGGCCTTTCCCGGGCGGTGGATCCCAA
GGAGTTCATGCGTTTGAGCGGGGTGCAGGAGGGAGCCAAGCGCCCCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.422

97.514

0.657
  pilT Acinetobacter baylyi ADP1

54.36

95.028

0.517
  pilT Pseudomonas aeruginosa PAK

54.07

95.028

0.514
  pilT Acinetobacter baumannii D1279779

53.913

95.304

0.514
  pilT Acinetobacter baumannii strain A118

53.913

95.304

0.514
  pilT Acinetobacter nosocomialis M2

53.913

95.304

0.514
  pilT Pseudomonas stutzeri DSM 10701

53.779

95.028

0.511
  pilT Neisseria meningitidis 8013

52.663

93.37

0.492
  pilT Legionella pneumophila strain Lp02

51.744

95.028

0.492
  pilT Legionella pneumophila strain ERS1305867

51.744

95.028

0.492
  pilT Neisseria gonorrhoeae MS11

51.929

93.094

0.483
  pilT Vibrio cholerae strain A1552

52.584

90.884

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.584

90.884

0.478
  pilU Pseudomonas stutzeri DSM 10701

47.522

94.751

0.45
  pilU Vibrio cholerae strain A1552

44.478

92.541

0.412
  pilU Acinetobacter baylyi ADP1

41.341

98.895

0.409