Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MI302_RS11350 Genome accession   NZ_CP092445
Coordinates   1983885..1985963 (+) Length   692 a.a.
NCBI ID   WP_370687348.1    Uniprot ID   -
Organism   Thermus sp. NEB1569     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1978885..1990963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MI302_RS11310 (MI302_11310) - 1979116..1979712 (-) 597 WP_239725832.1 sulfite oxidase-like oxidoreductase -
  MI302_RS11315 (MI302_11315) - 1979761..1980090 (-) 330 WP_126205059.1 DUF190 domain-containing protein -
  MI302_RS11320 (MI302_11320) ribH 1980175..1980648 (+) 474 WP_015716093.1 6,7-dimethyl-8-ribityllumazine synthase -
  MI302_RS11325 (MI302_11325) - 1980635..1981039 (-) 405 WP_026329111.1 DUF4395 family protein -
  MI302_RS11330 (MI302_11330) pgeF 1981119..1981844 (+) 726 WP_019551474.1 peptidoglycan editing factor PgeF -
  MI302_RS11335 (MI302_11335) - 1981876..1982364 (+) 489 WP_126178010.1 YqeG family HAD IIIA-type phosphatase -
  MI302_RS11340 (MI302_11340) - 1982378..1982962 (+) 585 Protein_2123 type II/IV secretion system protein -
  MI302_RS11345 (MI302_11345) - 1983018..1983863 (+) 846 WP_038032087.1 transposase -
  MI302_RS11350 (MI302_11350) pilF 1983885..1985963 (+) 2079 WP_370687348.1 type II/IV secretion system protein Machinery gene
  MI302_RS11355 (MI302_11355) pilT 1985975..1987063 (+) 1089 WP_239725833.1 type IV pilus twitching motility protein PilT Machinery gene
  MI302_RS11360 (MI302_11360) gatB 1987087..1988496 (+) 1410 WP_239725834.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  MI302_RS11365 (MI302_11365) purM 1988502..1989503 (+) 1002 WP_126200626.1 phosphoribosylformylglycinamidine cyclo-ligase -
  MI302_RS11370 (MI302_11370) - 1989500..1990129 (+) 630 WP_126169942.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 76758.55 Da        Isoelectric Point: 6.4612

>NTDB_id=657537 MI302_RS11350 WP_370687348.1 1983885..1985963(+) (pilF) [Thermus sp. NEB1569]
MGRILVRKGLSEEVLYQALSEQKGLEFLPSTEDLNPDPVATSLLLRSDALRYSAVPVAFRDGEVEVVLADPRHKEAVAEL
LGRPVRFLLTLPKEWEALFHQAYPEKSRLGEVLVQEGRLSREKLREALDVQRRLPKAKPLGEILVELGLARPEDVEEALK
KQRQGGGRLEDTLVASGKLKPEALAQAVAAQLGYAYINPEQTPPDPGAALLLPEDLCRRYGIFPHHVEGNRLVLLMKDPR
NILALDDVRLALKRKGLAYEVVPAVATEAAITKLIERFYSREELGEIAKELSKGYQEEEAVTPELDESAAQKFVRQVIRE
AYLQDASDIHIEPRQSDVLVRLRIDGTLRQYTTLSKGALNSIISVVKIMGGLNIAERRLPQDGRVRYREGAIDVDLRLST
LPTVYGEKAVMRLLKKAADIPEIEGLGFAPGVFERFQEVISKPYGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIED
PVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRDSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEM
GVELFNISAALIGVLSQRLVRRICDHCKVEVKPDPEVLHRLGLSEKELQGAKLFRGMGCERCGGTGYKGRYAIHELLVVD
DEIRHAIVAGKSATEIKEIARRKGMKTLREDGIQKVLQGITTLEEVLARTIE

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=657537 MI302_RS11350 WP_370687348.1 1983885..1985963(+) (pilF) [Thermus sp. NEB1569]
TTGGGCCGCATCCTGGTGCGAAAGGGGCTCTCCGAGGAGGTGCTATACCAGGCGCTTTCGGAGCAAAAGGGGCTTGAATT
CCTTCCCTCCACCGAAGACCTAAACCCCGACCCAGTGGCCACCTCCCTCCTCCTCCGCTCCGACGCCCTGCGCTATAGCG
CCGTGCCCGTAGCCTTCCGGGACGGGGAGGTGGAAGTGGTGCTGGCCGATCCCCGGCACAAGGAGGCGGTGGCGGAGCTC
CTGGGCCGGCCTGTCCGCTTCCTCCTCACCCTTCCCAAGGAGTGGGAAGCCCTTTTCCACCAGGCCTATCCGGAAAAAAG
CCGCCTGGGGGAGGTCCTGGTCCAGGAGGGGCGCCTGAGCCGGGAGAAGTTAAGGGAAGCCCTCGATGTGCAACGGCGCC
TACCCAAGGCCAAGCCCCTGGGGGAGATCCTGGTGGAGCTGGGCCTGGCCCGGCCTGAGGACGTGGAGGAGGCCTTAAAG
AAGCAACGCCAAGGCGGAGGCCGCCTGGAGGACACCCTGGTGGCCTCGGGGAAGTTAAAACCCGAGGCCTTGGCCCAGGC
GGTGGCGGCCCAGCTGGGCTACGCCTACATCAACCCTGAGCAGACCCCCCCTGACCCTGGGGCCGCCCTTCTCCTCCCCG
AGGACCTCTGCCGCCGCTACGGCATCTTCCCCCACCACGTGGAGGGAAATCGCCTAGTCCTCCTCATGAAGGACCCCCGG
AACATCCTGGCCCTGGACGATGTGCGCCTAGCCCTCAAGCGCAAGGGCCTAGCTTACGAGGTGGTGCCCGCCGTGGCCAC
GGAGGCCGCCATCACCAAGCTCATCGAACGCTTTTACAGCAGGGAGGAGCTGGGCGAGATCGCCAAGGAGCTATCCAAGG
GCTACCAGGAAGAGGAGGCCGTCACCCCCGAGCTGGACGAAAGCGCTGCCCAAAAGTTCGTCCGGCAGGTGATCCGGGAG
GCCTACCTCCAGGATGCCTCCGATATCCACATAGAGCCCAGGCAGTCGGACGTTCTGGTGCGCCTAAGGATTGACGGCAC
CCTGCGCCAGTACACCACTTTGTCCAAAGGGGCCCTAAACTCCATCATCAGCGTGGTCAAGATCATGGGCGGCCTCAACA
TCGCGGAAAGACGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGGGGCCATCGACGTGGACCTCCGCCTCTCCACC
CTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTTTTGAAAAAGGCCGCCGACATCCCCGAGATCGAGGGTCTGGG
CTTTGCCCCAGGCGTTTTTGAGCGCTTCCAGGAGGTGATCTCCAAGCCCTACGGCATCTTCCTCATCACCGGGCCCACGG
GCTCGGGTAAGAGCTTCACCACCTTCTCCATCCTAAAGCGCATCGCCACCCCCGACAAGAACACCCAGACCATCGAGGAT
CCCGTGGAGTACGAGATTCCTGGCATCAACCAGACCCAGGTGAACCCCCAGGCGGGCCTCACCTTCGCCCGGGCCCTGAG
GGCCTTTTTGAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGGGACTCAGAGACCGCCAAGATCGCCACCGAGG
CCGCCCTCACCGGGCATTTGGTCATCGCCACCCTGCACACCAACGATGCCGCCCAGGCCATTACCCGTCTGGACGAGATG
GGGGTGGAGCTCTTCAACATCTCCGCCGCCCTCATCGGGGTCCTTTCCCAGCGCCTGGTGCGAAGGATCTGCGACCACTG
CAAGGTGGAGGTGAAGCCCGATCCCGAGGTCCTGCACCGCCTGGGTCTGAGCGAAAAGGAGCTCCAGGGGGCCAAGCTCT
TTAGGGGCATGGGGTGCGAGCGTTGTGGCGGTACCGGGTACAAGGGACGCTACGCCATCCACGAGCTCTTGGTGGTGGAC
GACGAGATCCGCCACGCCATCGTGGCCGGCAAGTCGGCCACGGAGATCAAGGAGATCGCCCGCAGGAAGGGGATGAAGAC
CCTGCGGGAGGACGGCATCCAGAAGGTCCTCCAGGGGATTACCACCCTCGAGGAAGTCCTGGCGCGTACCATTGAGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(5-107)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(173-283)

Type II/IV secretion system protein (T2SSE)

(317-582)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

86.58

100

0.867
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

58.466

99.855

0.584