Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MJO52_RS01035 Genome accession   NZ_CP092418
Coordinates   217041..218075 (-) Length   344 a.a.
NCBI ID   WP_252084158.1    Uniprot ID   -
Organism   Microbulbifer variabilis strain SCSIO 43006     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 212041..223075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJO52_RS01010 (MJO52_01010) - 212327..213484 (+) 1158 WP_252084154.1 histidine kinase -
  MJO52_RS01015 (MJO52_01015) - 213481..214221 (+) 741 WP_252084155.1 LytTR family DNA-binding domain-containing protein -
  MJO52_RS01020 (MJO52_01020) - 214225..214833 (-) 609 WP_252084156.1 LysE family translocator -
  MJO52_RS01025 (MJO52_01025) - 214845..215594 (-) 750 WP_252084157.1 SDR family oxidoreductase -
  MJO52_RS01030 (MJO52_01030) pilU 215842..216993 (-) 1152 WP_152450611.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MJO52_RS01035 (MJO52_01035) pilT 217041..218075 (-) 1035 WP_252084158.1 type IV pilus twitching motility protein PilT Machinery gene
  MJO52_RS01040 (MJO52_01040) - 218145..218849 (+) 705 WP_252084159.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJO52_RS01045 (MJO52_01045) - 218922..219206 (+) 285 WP_252084160.1 hypothetical protein -
  MJO52_RS01050 (MJO52_01050) - 219266..221269 (-) 2004 WP_252084161.1 alpha/beta fold hydrolase -
  MJO52_RS01055 (MJO52_01055) proC 221391..222245 (+) 855 WP_252084162.1 pyrroline-5-carboxylate reductase -
  MJO52_RS01060 (MJO52_01060) - 222309..222917 (+) 609 WP_252084163.1 YggT family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38331.00 Da        Isoelectric Point: 6.5788

>NTDB_id=657315 MJO52_RS01035 WP_252084158.1 217041..218075(-) (pilT) [Microbulbifer variabilis strain SCSIO 43006]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPAMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTTLAAMMDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKAMVRSMLSESLQAVISQTLLKKNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGASVGMQTMDQCL
EDLVARRIVSREVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=657315 MJO52_RS01035 WP_252084158.1 217041..218075(-) (pilT) [Microbulbifer variabilis strain SCSIO 43006]
ATGGATATCACTGAACTCCTCGCCTTCAGCGCCAAACAAAACGCTTCGGATCTGCACCTCTCCGCAGGCCTTCCGCCGAT
GATCCGTGTGGATGGCGACGTGCGCCGTATCAACCTGCCGGCCATGGAACACAAGCAGGTGCACGGCCTGATCTACGAGA
TCATGAACGACAAGCAACGTAAGGACTACGAAGAATTTCTCGAGACCGACTTCTCTTTCGAGGTACCTGGTGTAGCCCGC
TTTCGTGTGAACGCCTTTAACCACAACCGCGGTGCCGGTGCCGTATTCCGGACCATTCCCTCCAAGGTTTTAACCATGGA
GGATCTGGGCATGGGCCAAGTGTTCAAACAAATCTCCGATACTCCGCGCGGCCTGGTCTTGGTCACAGGGCCCACCGGCT
CCGGTAAGTCCACCACCCTCGCCGCGATGATGGACTATATCAACGACAACAAATACGAGCATATCCTCACCATTGAGGAC
CCCATCGAATTCGTTCACGAATCCAAAAAATGCCTGGTGAACCAGCGCGAAGTGCATAGGGATACCCACGGCTTCGCAGA
AGCATTGCGCTCGGCCCTGCGTGAAGACCCGGATATCATTCTGGTGGGTGAGATGCGTGACCTGGAAACTATTCGCCTGG
CATTAACCGCAGCGGAAACCGGCCATTTGGTATTCGGCACCCTGCATACCACCTCCGCCGCCAAGACTATCGACAGGGTG
GTGGATGTGTTCCCGGCACAGGAAAAAGCCATGGTGCGCTCCATGCTATCGGAATCCCTGCAAGCGGTAATCTCGCAGAC
ACTGCTGAAGAAAAACGGCGGTGGCCGTGTCGCTGCCCACGAGATCATGCGCGGCACTCCCGCAATCCGCAACTTGATCC
GCGAAGACAAAATCGCGCAGATGTACTCCGCCATCCAGACTGGTGCCAGCGTTGGTATGCAGACCATGGACCAGTGCCTG
GAAGACCTGGTGGCACGGCGTATCGTCAGCCGCGAAGTAGCGCGGGAAAAAGCCAAGATGCCAGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

89.244

100

0.892
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

80.523

100

0.805
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.118

98.837

0.683
  pilT Vibrio cholerae strain A1552

69.118

98.837

0.683
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.547

0.401
  pilU Vibrio cholerae strain A1552

40.413

98.547

0.398
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387