Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MJO52_RS01030 Genome accession   NZ_CP092418
Coordinates   215842..216993 (-) Length   383 a.a.
NCBI ID   WP_152450611.1    Uniprot ID   -
Organism   Microbulbifer variabilis strain SCSIO 43006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 210842..221993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJO52_RS01010 (MJO52_01010) - 212327..213484 (+) 1158 WP_252084154.1 histidine kinase -
  MJO52_RS01015 (MJO52_01015) - 213481..214221 (+) 741 WP_252084155.1 LytTR family DNA-binding domain-containing protein -
  MJO52_RS01020 (MJO52_01020) - 214225..214833 (-) 609 WP_252084156.1 LysE family translocator -
  MJO52_RS01025 (MJO52_01025) - 214845..215594 (-) 750 WP_252084157.1 SDR family oxidoreductase -
  MJO52_RS01030 (MJO52_01030) pilU 215842..216993 (-) 1152 WP_152450611.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MJO52_RS01035 (MJO52_01035) pilT 217041..218075 (-) 1035 WP_252084158.1 type IV pilus twitching motility protein PilT Machinery gene
  MJO52_RS01040 (MJO52_01040) - 218145..218849 (+) 705 WP_252084159.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJO52_RS01045 (MJO52_01045) - 218922..219206 (+) 285 WP_252084160.1 hypothetical protein -
  MJO52_RS01050 (MJO52_01050) - 219266..221269 (-) 2004 WP_252084161.1 alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42620.96 Da        Isoelectric Point: 6.5035

>NTDB_id=657314 MJO52_RS01030 WP_152450611.1 215842..216993(-) (pilU) [Microbulbifer variabilis strain SCSIO 43006]
MDIERLLQLVVEKGASDLFITAGVPPSIKLHGRVVPCSSSALTPEKARELVVGTMNEKQRREFAEKKELNFAIAVRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPDVLKDLAMTKRGLIIFVGATGTGKSTSLASMIGHRNENTKGHIITIED
PIEFIHQHRGCIVTQREVGLDTDSFEIALKNTLRQAPDVILIGEVRSRETMEHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLFMDLSLNLKAMVAQQLVPTPDGDGRRACLEIMINTPLMSDLVRKGQVHKMKELMKRSGEQGMQTFDQA
LYELYDRGEITYEDALAHADSANDLRLMIKLKSESDAEYLNSAAGELSLQNDSEYGDGGPQLY

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=657314 MJO52_RS01030 WP_152450611.1 215842..216993(-) (pilU) [Microbulbifer variabilis strain SCSIO 43006]
ATGGATATAGAAAGACTCCTACAGCTGGTAGTAGAGAAAGGTGCATCGGATCTGTTTATCACCGCCGGCGTACCGCCTTC
CATTAAACTGCACGGCCGCGTGGTGCCTTGCAGTAGCAGTGCCCTCACCCCAGAAAAAGCCCGCGAGCTAGTAGTGGGCA
CCATGAATGAGAAACAGCGCCGTGAGTTCGCCGAGAAGAAGGAATTAAACTTCGCCATTGCGGTACGCAACGTGGGCCGT
TTCCGGGTGTCGGCTTTCTTCCAGCGCAACTTGGTGGGTATGGTGCTGCGACGCATTGAAACAAAAATCCCCACCATTGA
CGGCCTCGGCCTGCCGGATGTACTCAAAGATCTGGCCATGACCAAGCGCGGGCTGATTATTTTTGTAGGTGCCACCGGTA
CCGGTAAGTCCACCTCCCTGGCCTCGATGATCGGCCACCGCAATGAGAACACCAAGGGTCATATCATCACCATTGAGGAC
CCGATCGAATTTATCCACCAGCACCGCGGCTGTATTGTGACCCAGCGCGAAGTGGGTCTGGATACCGACTCCTTCGAAAT
CGCCTTGAAGAACACTCTGCGTCAAGCGCCAGATGTCATCCTGATTGGTGAGGTGCGCTCCCGCGAAACGATGGAGCACG
CCATTGCTTTCGCCGAAACTGGTCACCTGTGCCTGTGTACACTGCACGCCAACAATGCCAACCAAGCCCTCGACCGGATC
ATTCACTTCTTCCCTGCAGACCGTCACCAACAGCTGTTTATGGATCTGTCCCTGAACCTTAAGGCCATGGTTGCCCAGCA
GCTGGTACCAACACCGGATGGCGATGGCCGCCGCGCCTGTCTGGAAATTATGATCAACACGCCGTTGATGTCAGACCTGG
TGCGCAAAGGCCAGGTGCACAAGATGAAGGAGTTGATGAAACGCTCCGGCGAACAGGGCATGCAGACCTTCGACCAGGCG
CTGTATGAACTCTACGATCGCGGCGAAATTACTTACGAGGATGCCCTCGCCCACGCCGACTCCGCCAACGACTTGCGCCT
GATGATCAAGCTTAAGTCTGAATCCGATGCGGAGTATTTAAACAGCGCTGCCGGTGAGTTGAGTCTACAAAATGACTCTG
AGTACGGCGATGGTGGGCCGCAACTTTACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

74.595

96.606

0.721
  pilU Acinetobacter baylyi ADP1

67.507

93.211

0.629
  pilU Vibrio cholerae strain A1552

54.717

96.867

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.018

88.512

0.407
  pilT Acinetobacter nosocomialis M2

43.881

87.467

0.384
  pilT Acinetobacter baumannii D1279779

43.881

87.467

0.384
  pilT Acinetobacter baumannii strain A118

43.881

87.467

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.515

87.206

0.371
  pilT Vibrio cholerae strain A1552

42.515

87.206

0.371
  pilT Acinetobacter baylyi ADP1

41.642

89.034

0.371
  pilT Pseudomonas aeruginosa PAK

42.09

87.467

0.368
  pilT Legionella pneumophila strain Lp02

41.791

87.467

0.366
  pilT Legionella pneumophila strain ERS1305867

41.791

87.467

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.493

87.467

0.363