Detailed information    

experimental Experimentally validated

Overview

Name   recR   Type   Machinery gene
Locus tag   SPR_RS07520 Genome accession   NC_003098
Coordinates   1493609..1494205 (-) Length   198 a.a.
NCBI ID   WP_000966743.1    Uniprot ID   P0CB76
Organism   Streptococcus pneumoniae R6     
Function   homologous recombination   
Homologous recombination

Function

A ∼1.45-fold drop in transformation was observed with total chromosomal DNA in recFOR mutants. Transformation with chromosomal DNA stimulated unexpectedly high frequency (>30% of cells) formation of chromosome dimers as an intermediate in the generation of tandem duplications, and that RecFOR were crucial for dimer resolution. The site-specific recombinase XerS was also crucial for dimer resolution.


Genomic Context

Location: 1488609..1499205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS07500 (spr1512) - 1489540..1490244 (-) 705 WP_001055251.1 TIGR02206 family membrane protein -
  SPR_RS07505 (spr1513) - 1490335..1490946 (-) 612 WP_000857991.1 NUDIX hydrolase N-terminal domain-containing protein -
  SPR_RS07510 (spr1514) murF 1490933..1492306 (-) 1374 WP_000778688.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
  SPR_RS07515 (spr1515) - 1492390..1493433 (-) 1044 WP_000814630.1 D-alanine--D-alanine ligase -
  SPR_RS07520 (spr1516) recR 1493609..1494205 (-) 597 WP_000966743.1 recombination mediator RecR Machinery gene
  SPR_RS07525 (spr1517) pbp2b 1494216..1496273 (-) 2058 WP_001829432.1 penicillin-binding protein 2B -
  SPR_RS07530 (spr1518) - 1496473..1497324 (+) 852 WP_000360342.1 MurR/RpiR family transcriptional regulator -
  SPR_RS07535 (spr1519) - 1497340..1498224 (-) 885 WP_000222983.1 ROK family protein -
  SPR_RS07540 (spr1520) - 1498242..1499159 (-) 918 Protein_1489 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 21688.86 Da        Isoelectric Point: 4.5622

>NTDB_id=652 SPR_RS07520 WP_000966743.1 1493609..1494205(-) (recR) [Streptococcus pneumoniae R6]
MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFAKNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQT
TILVLEDSRDVAAMENIQEYHGLYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL

Nucleotide


Download         Length: 597 bp        

>NTDB_id=652 SPR_RS07520 WP_000966743.1 1493609..1494205(-) (recR) [Streptococcus pneumoniae R6]
ATGCTTTATCCAACACCTATTGCCAAGTTGATTGACAGTTATTCTAAGTTACCAGGTATCGGGATTAAGACGGCTACGCG
TCTGGCCTTTTATACGATTGGGATGTCTGCTGATGATGTCAATGAATTTGCAAAAAATCTCCTTTCTGCTAAGAGAGAAT
TGACATATTGTTCTATTTGTGGACGTTTGACAGACGACGATCCTTGTTCTATCTGTACTGATCCGACTCGTGACCAGACA
ACAATTTTAGTTCTTGAGGATAGTAGAGATGTGGCAGCCATGGAAAATATCCAAGAATACCATGGACTCTATCATGTCCT
TCATGGCCTCATTTCTCCTATGAATGGTATCAGTCCGGACGATATCAATCTCAAGAGCCTTATGACTCGTCTTATGGATA
GTGAGGTTTCAGAAGTGATTGTGGCGACTAATGCTACAGCGGATGGTGAAGCGACTTCCATGTATCTTTCACGTTTGCTC
AAGCCGGCTGGTATCAAGGTTACGCGTCTAGCACGAGGTCTCGCTGTGGGAGCGGACATTGAGTATGCGGACGAAGTGAC
ACTCTTACGAGCCATTGAAAATCGGACAGAGTTGTAA

Domains

Predicted by InterProScan.

RecR protein (RecR_ZnF)

(39-78)

Toprim domain (Toprim_4)

(81-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0CB76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recR Bacillus subtilis subsp. subtilis str. 168

62.121

100

0.621
  recR Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.231

98.485

0.485

References

[1] Vanessa Khemici et al. (2021) Tight Interplay between Replication Stress and Competence Induction in Streptococcus pneumoniae. Cells 10(8):1938. [PMID: 34440707]
[2] Calum Johnston et al. (2015) RecFOR is not required for pneumococcal transformation but together with XerS for resolution of chromosome dimers frequently formed in the process. PLoS Genetics 11(1):e1004934. [PMID: 25569614]