Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MJO57_RS31755 Genome accession   NZ_CP092417
Coordinates   7025597..7026631 (-) Length   344 a.a.
NCBI ID   WP_252021599.1    Uniprot ID   -
Organism   Endozoicomonas sp. SCSIO W0465     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7020597..7031631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJO57_RS31730 (MJO57_31600) gshB 7021276..7022232 (+) 957 WP_252027153.1 glutathione synthase -
  MJO57_RS31735 (MJO57_31605) - 7022469..7023290 (+) 822 WP_252021590.1 energy transducer TonB -
  MJO57_RS31740 (MJO57_31610) - 7023386..7023958 (+) 573 WP_252021592.1 YqgE/AlgH family protein -
  MJO57_RS31745 (MJO57_31615) ruvX 7023978..7024427 (+) 450 WP_252021594.1 Holliday junction resolvase RuvX -
  MJO57_RS31750 (MJO57_31620) pilU 7024472..7025596 (-) 1125 WP_252021596.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MJO57_RS31755 (MJO57_31625) pilT 7025597..7026631 (-) 1035 WP_252021599.1 type IV pilus twitching motility protein PilT Machinery gene
  MJO57_RS31760 (MJO57_31630) - 7026698..7027429 (+) 732 WP_252021601.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJO57_RS31765 (MJO57_31635) proC 7027435..7028256 (+) 822 WP_252021603.1 pyrroline-5-carboxylate reductase -
  MJO57_RS31770 (MJO57_31640) - 7028376..7028930 (+) 555 WP_256492933.1 YggT family protein -
  MJO57_RS31775 (MJO57_31645) - 7029064..7031538 (+) 2475 WP_252021607.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38058.89 Da        Isoelectric Point: 7.0289

>NTDB_id=657310 MJO57_RS31755 WP_252021599.1 7025597..7026631(-) (pilT) [Endozoicomonas sp. SCSIO W0465]
MDITELLAFSFKQGASDLHLSAGLPPIIRIDGDVRRINLPAMDQKEVHGLIYDIMNDKQRKDLEEHFEADFSFEVPGVAR
FRVNAFNQNRGAGAVFRAIPSKVLTMDDLGMGQVFKTIADKPRGLVLVTGPTGSGKSTTLAAMMDYINDTKYQHILTIED
PIEFIHIPKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAEEKSMVRSMLSESLQAVISQTLLKKNGGGRVAAHEIMLGTAGIRNLIREDKVAQMYSAIQTGGGLGMQTLDQCL
MNLIKKGLISRDVAREKAKDRDAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=657310 MJO57_RS31755 WP_252021599.1 7025597..7026631(-) (pilT) [Endozoicomonas sp. SCSIO W0465]
ATGGATATTACTGAGCTATTGGCGTTCAGCTTTAAACAGGGGGCTTCGGATCTGCATCTGTCGGCAGGGCTTCCGCCAAT
TATCCGAATCGATGGTGATGTTCGCCGTATCAATCTGCCAGCCATGGATCAGAAGGAAGTTCATGGGTTGATCTATGACA
TCATGAACGACAAACAGCGTAAGGATCTGGAAGAGCATTTCGAAGCGGACTTCTCGTTTGAAGTCCCCGGCGTTGCGCGT
TTCCGTGTGAATGCTTTCAACCAGAACAGGGGTGCGGGAGCGGTGTTCAGGGCAATCCCCAGTAAAGTACTGACCATGGA
TGACCTGGGCATGGGGCAGGTATTTAAAACCATTGCGGATAAACCCAGGGGGTTAGTGCTGGTGACTGGTCCTACCGGCT
CTGGCAAGAGTACCACACTGGCTGCCATGATGGATTACATTAATGACACCAAATATCAGCATATCCTGACCATTGAAGAT
CCCATTGAATTTATCCACATCCCGAAAAAGTGCCTGGTTAATCAGCGGGAAGTGCATCGGGATACCCATAGCTTCAGTAA
TGCCCTGCGCTCTGCGTTGCGTGAAGATCCTGATATTATTCTCGTCGGTGAGATGCGTGACCTGGAAACAATCCGTCTGG
CCATGACGGCGGCTGAAACCGGTCATCTGGTGTTCGGAACGTTGCACACCACATCGGCTGCAAAGACCATTGACCGTATC
ATCGATGTATTCCCGGCTGAAGAAAAGTCGATGGTACGTTCAATGCTCTCTGAGTCACTCCAGGCCGTGATTTCGCAAAC
ACTGTTGAAGAAAAATGGCGGAGGGCGTGTGGCCGCACATGAGATTATGCTCGGTACTGCAGGTATTCGTAATCTGATTC
GTGAAGATAAGGTTGCGCAGATGTACTCGGCCATTCAGACCGGCGGTGGGCTTGGCATGCAAACATTGGATCAGTGCCTC
ATGAACCTGATAAAAAAGGGACTGATCTCTCGTGATGTGGCTCGTGAAAAGGCCAAGGATCGAGACGCATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

86.047

100

0.86
  pilT Pseudomonas aeruginosa PAK

84.593

100

0.846
  pilT Acinetobacter nosocomialis M2

80.233

100

0.802
  pilT Acinetobacter baumannii D1279779

80.233

100

0.802
  pilT Acinetobacter baumannii strain A118

80.233

100

0.802
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

72.965

100

0.73
  pilT Legionella pneumophila strain ERS1305867

72.965

100

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.246

100

0.674
  pilT Vibrio cholerae strain A1552

67.246

100

0.674
  pilT Neisseria meningitidis 8013

64.928

100

0.651
  pilT Neisseria gonorrhoeae MS11

64.348

100

0.645
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

42.478

98.547

0.419
  pilU Vibrio cholerae strain A1552

41.39

96.221

0.398
  pilU Acinetobacter baylyi ADP1

40.663

96.512

0.392