Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MJO57_RS31750 Genome accession   NZ_CP092417
Coordinates   7024472..7025596 (-) Length   374 a.a.
NCBI ID   WP_252021596.1    Uniprot ID   -
Organism   Endozoicomonas sp. SCSIO W0465     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7019472..7030596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJO57_RS31725 (MJO57_31595) - 7020196..7021104 (+) 909 WP_252021588.1 TauD/TfdA family dioxygenase -
  MJO57_RS31730 (MJO57_31600) gshB 7021276..7022232 (+) 957 WP_252027153.1 glutathione synthase -
  MJO57_RS31735 (MJO57_31605) - 7022469..7023290 (+) 822 WP_252021590.1 energy transducer TonB -
  MJO57_RS31740 (MJO57_31610) - 7023386..7023958 (+) 573 WP_252021592.1 YqgE/AlgH family protein -
  MJO57_RS31745 (MJO57_31615) ruvX 7023978..7024427 (+) 450 WP_252021594.1 Holliday junction resolvase RuvX -
  MJO57_RS31750 (MJO57_31620) pilU 7024472..7025596 (-) 1125 WP_252021596.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MJO57_RS31755 (MJO57_31625) pilT 7025597..7026631 (-) 1035 WP_252021599.1 type IV pilus twitching motility protein PilT Machinery gene
  MJO57_RS31760 (MJO57_31630) - 7026698..7027429 (+) 732 WP_252021601.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJO57_RS31765 (MJO57_31635) proC 7027435..7028256 (+) 822 WP_252021603.1 pyrroline-5-carboxylate reductase -
  MJO57_RS31770 (MJO57_31640) - 7028376..7028930 (+) 555 WP_256492933.1 YggT family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 42081.73 Da        Isoelectric Point: 7.6039

>NTDB_id=657309 MJO57_RS31750 WP_252021596.1 7024472..7025596(-) (pilU) [Endozoicomonas sp. SCSIO W0465]
MDIEKLLKLVVVKKASDLFITAGFPPSVKLDGRLKPVTRTPLTATETKNMVLGVMDEKQRLEFETKQESNFAIARMGIGR
FRVSAFQQRNQSGMVLRRIEEKIPRLDDLRLPAVLKDLTMARRGLVLVVGATGTGKSTTLAAMLGYRNENDTGHIISIED
PIEFIHQHKKCIVTQREVGIDTRSYEVALKNTLRQAPDVIMIGEIRSQDTMQYALNFAETGHLCLATLHANNANQALDRI
LNFFPPEHHHQIWMDLSLNLKGIVAQQLVPSLDGTGRLPVIEVLLNTPLAKDMIRNGDVHALKELMKKSRELGMQTFDQA
LFDLYNEGEVSHEEAIRHADSANDLRLMIKLGSEVNERYLTERSVKLILQMEGG

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=657309 MJO57_RS31750 WP_252021596.1 7024472..7025596(-) (pilU) [Endozoicomonas sp. SCSIO W0465]
ATGGATATTGAAAAATTATTAAAGTTGGTCGTCGTTAAAAAAGCATCGGATCTGTTTATCACCGCAGGTTTTCCGCCCAG
TGTAAAGCTTGATGGACGTCTCAAGCCTGTCACCAGGACACCGCTGACTGCCACAGAGACAAAAAATATGGTGCTGGGTG
TGATGGACGAGAAGCAGCGTCTTGAGTTTGAGACTAAGCAGGAGTCTAACTTTGCCATTGCCAGAATGGGGATTGGTCGG
TTCCGGGTCAGTGCTTTTCAACAGAGAAACCAGTCAGGCATGGTTTTGCGCCGCATTGAAGAGAAAATTCCCAGACTCGA
CGACCTGAGGCTTCCCGCTGTTCTTAAAGACCTGACCATGGCCAGGCGCGGCCTTGTCCTGGTGGTGGGTGCTACCGGTA
CGGGCAAGTCGACAACGTTGGCAGCGATGCTTGGCTATCGTAATGAAAACGACACTGGCCATATTATCAGTATTGAAGAC
CCCATTGAATTTATCCATCAACACAAAAAGTGCATCGTAACCCAGCGGGAAGTGGGGATTGATACCAGGTCCTATGAAGT
GGCGTTGAAAAACACCCTTCGTCAGGCTCCTGACGTCATTATGATCGGTGAGATTCGAAGCCAGGATACTATGCAATATG
CCCTGAACTTCGCAGAAACCGGCCATCTCTGCCTGGCAACACTTCATGCCAATAATGCCAACCAGGCATTGGATCGCATT
CTGAATTTTTTCCCCCCGGAACATCATCACCAGATCTGGATGGATCTTTCCCTTAATCTGAAGGGCATTGTTGCCCAGCA
GCTGGTTCCTTCCCTGGATGGCACCGGTCGGCTTCCGGTTATTGAAGTGCTACTCAATACACCGCTAGCCAAGGATATGA
TTCGCAACGGGGATGTTCATGCACTGAAAGAGTTAATGAAAAAATCCCGGGAGCTGGGCATGCAGACGTTCGACCAGGCA
TTATTTGATCTCTACAACGAAGGGGAGGTCAGCCATGAAGAAGCGATTCGGCATGCTGATTCGGCAAACGACTTGCGCCT
GATGATTAAACTGGGTTCAGAAGTGAATGAGCGTTATCTTACCGAACGTTCCGTTAAATTGATCCTGCAGATGGAAGGAG
GATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.003

99.198

0.684
  pilU Acinetobacter baylyi ADP1

60.056

95.722

0.575
  pilU Vibrio cholerae strain A1552

57.714

93.583

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

90.642

0.38
  pilT Legionella pneumophila strain Lp02

40.588

90.909

0.369
  pilT Legionella pneumophila strain ERS1305867

40.588

90.909

0.369
  pilT Pseudomonas aeruginosa PAK

40.597

89.572

0.364
  pilT Acinetobacter nosocomialis M2

40.299

89.572

0.361
  pilT Acinetobacter baumannii D1279779

40.299

89.572

0.361
  pilT Acinetobacter baumannii strain A118

40.299

89.572

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

89.305

0.361
  pilT Vibrio cholerae strain A1552

40.419

89.305

0.361