Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MID13_RS13315 Genome accession   NZ_CP092384
Coordinates   2990295..2991332 (-) Length   345 a.a.
NCBI ID   WP_048605840.1    Uniprot ID   A0AA86X1P3
Organism   Vibrio gigantis strain ACE001     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2985295..2996332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID13_RS13285 (MID13_13285) rsmE 2985989..2986720 (+) 732 WP_215406815.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MID13_RS13290 (MID13_13290) gshB 2986730..2987680 (+) 951 WP_239716681.1 glutathione synthase -
  MID13_RS13295 (MID13_13295) - 2987790..2988359 (+) 570 WP_239716683.1 YqgE/AlgH family protein -
  MID13_RS13300 (MID13_13300) ruvX 2988413..2988835 (+) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  MID13_RS13305 (MID13_13305) - 2988935..2989096 (+) 162 WP_167373144.1 hypothetical protein -
  MID13_RS13310 (MID13_13310) pilU 2989171..2990280 (-) 1110 WP_239716685.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MID13_RS13315 (MID13_13315) pilT 2990295..2991332 (-) 1038 WP_048605840.1 type IV pilus twitching motility protein PilT Machinery gene
  MID13_RS13320 (MID13_13320) - 2991359..2992063 (+) 705 WP_086712020.1 YggS family pyridoxal phosphate-dependent enzyme -
  MID13_RS13325 (MID13_13325) proC 2992148..2992966 (+) 819 WP_239716687.1 pyrroline-5-carboxylate reductase -
  MID13_RS13330 (MID13_13330) - 2993024..2993581 (+) 558 WP_010435653.1 YggT family protein -
  MID13_RS13335 (MID13_13335) yggU 2993611..2993901 (+) 291 WP_086712022.1 DUF167 family protein YggU -
  MID13_RS13340 (MID13_13340) - 2993930..2994361 (+) 432 WP_016768173.1 DUF4426 domain-containing protein -
  MID13_RS13345 (MID13_13345) - 2994408..2995007 (+) 600 WP_215406812.1 XTP/dITP diphosphatase -
  MID13_RS13350 (MID13_13350) hemW 2995007..2996194 (+) 1188 WP_239716689.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38243.79 Da        Isoelectric Point: 6.0512

>NTDB_id=657100 MID13_RS13315 WP_048605840.1 2990295..2991332(-) (pilT) [Vibrio gigantis strain ACE001]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLEQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=657100 MID13_RS13315 WP_048605840.1 2990295..2991332(-) (pilT) [Vibrio gigantis strain ACE001]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATCCCAGCTTTGAGTCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAGTTTGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGC
TTCCGTGTGAATGCTTTTAACCAAGCTCGTGGTTGCTCGGCTGTCTTTCGAACTATCCCTGTAGAGATCCCAACTTTAGA
ACAGTTAGGTGCGCCTGAAATCTTTGAAAAGATTGCGAACTACGAAAAAGGTTTGGTACTTGTCACTGGTCCCACTGGTT
CTGGTAAATCGACGACGCTCGCGGCGATGGTGGATTACGTAAACCGTAACCACAATAAGCACATCCTGACCATCGAAGAC
CCGATTGAATTCGTTCATACCAACAATAAATGCCTTGTTAACCAGCGTGAGGTTCATCGTGATACTCACAGCTTTAAAGC
GGCGCTACGCAGCGCGTTACGTGAAGATCCAGACGTTATCCTTGTGGGTGAGCTTCGTGACCAAGAGACGATCAGCTTAG
CGCTAACCGCAGCGGAAACCGGCCACTTGGTTTTTGGTACTTTGCATACTAGCTCTGCGGCTAAAACTATCGACCGTATT
ATCGATGTATTCCCGGGGAGCGATAAAGATATGGTGCGTTCTATGCTGTCTGAATCGTTACGTTCGGTCATCGCTCAGAA
ACTATTGAAGCGTGTAGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCGATCAGAAACTTGATTC
GTGAAGACAAGGTCGCGCAGATGTATTCGATCATTCAAACGGGGGCGGCACATGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGCTGATGGCGCAAGGCTTGGTTGACTCGGAAGAGGTCGAGAAAAAAATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

89.275

100

0.893
  pilT Vibrio cholerae strain A1552

89.275

100

0.893
  pilT Acinetobacter baumannii D1279779

72.289

96.232

0.696
  pilT Acinetobacter baumannii strain A118

72.289

96.232

0.696
  pilT Acinetobacter nosocomialis M2

71.988

96.232

0.693
  pilT Pseudomonas aeruginosa PAK

69.118

98.551

0.681
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Acinetobacter baylyi ADP1

71.212

95.652

0.681
  pilT Legionella pneumophila strain Lp02

65.797

100

0.658
  pilT Legionella pneumophila strain ERS1305867

65.797

100

0.658
  pilT Neisseria meningitidis 8013

62.899

100

0.629
  pilT Neisseria gonorrhoeae MS11

62.609

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

43.385

94.203

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.812

0.397
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368