Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MID13_RS13310 Genome accession   NZ_CP092384
Coordinates   2989171..2990280 (-) Length   369 a.a.
NCBI ID   WP_239716685.1    Uniprot ID   -
Organism   Vibrio gigantis strain ACE001     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2984171..2995280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID13_RS13280 (MID13_13280) - 2984451..2985794 (-) 1344 WP_239716679.1 hypothetical protein -
  MID13_RS13285 (MID13_13285) rsmE 2985989..2986720 (+) 732 WP_215406815.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MID13_RS13290 (MID13_13290) gshB 2986730..2987680 (+) 951 WP_239716681.1 glutathione synthase -
  MID13_RS13295 (MID13_13295) - 2987790..2988359 (+) 570 WP_239716683.1 YqgE/AlgH family protein -
  MID13_RS13300 (MID13_13300) ruvX 2988413..2988835 (+) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  MID13_RS13305 (MID13_13305) - 2988935..2989096 (+) 162 WP_167373144.1 hypothetical protein -
  MID13_RS13310 (MID13_13310) pilU 2989171..2990280 (-) 1110 WP_239716685.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MID13_RS13315 (MID13_13315) pilT 2990295..2991332 (-) 1038 WP_048605840.1 type IV pilus twitching motility protein PilT Machinery gene
  MID13_RS13320 (MID13_13320) - 2991359..2992063 (+) 705 WP_086712020.1 YggS family pyridoxal phosphate-dependent enzyme -
  MID13_RS13325 (MID13_13325) proC 2992148..2992966 (+) 819 WP_239716687.1 pyrroline-5-carboxylate reductase -
  MID13_RS13330 (MID13_13330) - 2993024..2993581 (+) 558 WP_010435653.1 YggT family protein -
  MID13_RS13335 (MID13_13335) yggU 2993611..2993901 (+) 291 WP_086712022.1 DUF167 family protein YggU -
  MID13_RS13340 (MID13_13340) - 2993930..2994361 (+) 432 WP_016768173.1 DUF4426 domain-containing protein -
  MID13_RS13345 (MID13_13345) - 2994408..2995007 (+) 600 WP_215406812.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41367.24 Da        Isoelectric Point: 5.8700

>NTDB_id=657099 MID13_RS13310 WP_239716685.1 2989171..2990280(-) (pilU) [Vibrio gigantis strain ACE001]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNAAQVAQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTSALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=657099 MID13_RS13310 WP_239716685.1 2989171..2990280(-) (pilU) [Vibrio gigantis strain ACE001]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTGTCTCAAAAGGCGTCGGATCTTTATATCACCGTTGATGCGCCTATCTT
GTTTCGTGTTGATGGTGAATTGCGACCTCAAGGCGAGAAGCTGAATGCGGCTCAGGTTGCTCAACTACTTGATGCGATGA
TGGATCAAGACAGACGTGATGAATATCAGCAAACACGTGAGGCAAACTTTGCGATTGTGCGTGATTTTGGTCGTTTTCGT
GTCAGTGCGTTTTTTCAGCGAGAGCTACCAGGAGCGGTAATTCGTCGTATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTACTGCAAGACCTTTCAATCGCAAAGCGTGGGCTGGTGCTGGTGGTTGGAGCGACCGGCTCTGGTA
AGTCGACCTCAATGGCTGCAATGACGGGTTACCGTAATACCAATCGCTCGGGGCATATCTTGACGGTTGAAGACCCGATT
GAATTTGTTCACGAACACAAGAAATGTATCGTGACTCAGCGCGAGGTAGGGCTTGATACAGAGAGCTATGAAGTCGCACT
TAAGAACTCGTTACGCCAAGCGCCAGATATGATTCTGATTGGTGAAATCCGTAGCCGTGAAACCATGGAATATGCGATGA
CCTTTGCTGAGACGGGTCACCTGTGTATGGCAACTTTGCACGCGAATAATGCCAACCAAGCGCTAGAGCGTATTCTCCAC
TTGGTGCCGAAAGAACAGAAAGAACAGTTCCTGTTTGACCTGTCGATGAACCTGCGTGGTGTGGTTGCTCAGCAGTTAAT
CAGAGACAAGAATGGCAGTGGGCGTCATGGTGTGTTTGAGATTCTACTGAACAGCCCACGCGTGTCTGATTTGATTCGTC
GCGGTGAGTTGCATGAGTTAAAAGCAACGATGGCAAAATCGAAAGAGATTGGTATGCAGACCTTTGATCAAGCTTTGTAT
GATTTAGTTGTTGCAGGCAAGATCAGCGAAGAAGACGCGTTCCACAGTGCCGATTCGGCGAATGATTTGCGCTTAATGCT
CAAAACCAGACGTGGAGATGATGACTACGGAACGAGTGCATTGGCCGGTGTGAAGATTGACATGGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.511

99.729

0.843
  pilU Pseudomonas stutzeri DSM 10701

56.604

100

0.569
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Acinetobacter baylyi ADP1

42.169

89.973

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363