Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MF133_RS15615 Genome accession   NZ_CP092181
Coordinates   3365126..3366160 (-) Length   344 a.a.
NCBI ID   WP_010673050.1    Uniprot ID   A0A081LS93
Organism   Aeromonas caviae strain 211703     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3321797..3388507 3365126..3366160 within 0


Gene organization within MGE regions


Location: 3321797..3388507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF133_RS15415 (MF133_15415) rlmD 3322559..3323911 (+) 1353 WP_043152994.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  MF133_RS15420 (MF133_15420) - 3324099..3324608 (+) 510 WP_239054223.1 GNAT family N-acetyltransferase -
  MF133_RS15425 (MF133_15425) - 3324690..3324860 (+) 171 WP_167418745.1 DUF5363 family protein -
  MF133_RS15430 (MF133_15430) tppF 3324829..3325443 (-) 615 WP_029313748.1 type IVa pilus pseudopilin TppF -
  MF133_RS15435 (MF133_15435) - 3325484..3326272 (+) 789 WP_041214451.1 TatD family hydrolase -
  MF133_RS15440 (MF133_15440) - 3326486..3327760 (+) 1275 WP_201871602.1 NupC/NupG family nucleoside CNT transporter -
  MF133_RS15445 (MF133_15445) - 3327884..3328054 (+) 171 WP_010673027.1 DUF1427 family protein -
  MF133_RS15450 (MF133_15450) deoC 3328442..3329215 (+) 774 WP_039039946.1 deoxyribose-phosphate aldolase -
  MF133_RS15455 (MF133_15455) deoA 3329298..3330629 (+) 1332 WP_239052873.1 thymidine phosphorylase -
  MF133_RS15460 (MF133_15460) - 3330644..3331852 (+) 1209 WP_039039948.1 phosphopentomutase -
  MF133_RS15465 (MF133_15465) deoD 3331986..3332702 (+) 717 WP_005341325.1 purine-nucleoside phosphorylase -
  MF133_RS15470 (MF133_15470) - 3332767..3335013 (-) 2247 WP_239052874.1 PAS domain S-box protein -
  MF133_RS15475 (MF133_15475) - 3335079..3335687 (-) 609 WP_010673032.1 AhpA/YtjB family protein -
  MF133_RS15480 (MF133_15480) serB 3335881..3336885 (+) 1005 WP_239052875.1 phosphoserine phosphatase SerB -
  MF133_RS15485 (MF133_15485) - 3336914..3337819 (+) 906 WP_043153013.1 alpha/beta fold hydrolase -
  MF133_RS15490 (MF133_15490) - 3337876..3340251 (-) 2376 WP_239052876.1 PilZ domain-containing protein -
  MF133_RS15495 (MF133_15495) radA 3340352..3341716 (+) 1365 WP_010673036.1 DNA repair protein RadA -
  MF133_RS15500 (MF133_15500) - 3341770..3342156 (-) 387 WP_029313755.1 PilZ domain-containing protein -
  MF133_RS15505 (MF133_15505) - 3342259..3342741 (-) 483 WP_029313757.1 Rsd/AlgQ family anti-sigma factor -
  MF133_RS15510 (MF133_15510) hupA 3343080..3343352 (+) 273 WP_005305063.1 nucleoid-associated protein HU-alpha -
  MF133_RS15515 (MF133_15515) - 3343466..3343891 (+) 426 WP_029313758.1 hypothetical protein -
  MF133_RS15520 (MF133_15520) - 3343974..3344123 (-) 150 WP_080944170.1 hypothetical protein -
  MF133_RS15525 (MF133_15525) dusA 3344126..3345082 (-) 957 WP_239052877.1 tRNA dihydrouridine(20/20a) synthase DusA -
  MF133_RS15530 (MF133_15530) - 3345267..3346367 (+) 1101 WP_239052878.1 site-specific integrase -
  MF133_RS15535 (MF133_15535) - 3346527..3347567 (+) 1041 Protein_3001 IS481 family transposase -
  MF133_RS15540 (MF133_15540) - 3347717..3351787 (-) 4071 WP_239052879.1 hypothetical protein -
  MF133_RS15545 (MF133_15545) mobI 3352348..3352923 (+) 576 WP_239052880.1 conjugative transfer protein MobI(A/C) -
  MF133_RS15550 (MF133_15550) - 3353579..3354436 (+) 858 WP_239052881.1 hypothetical protein -
  MF133_RS15555 (MF133_15555) - 3354711..3355058 (+) 348 WP_239052882.1 hypothetical protein -
  MF133_RS15560 (MF133_15560) - 3355236..3355394 (-) 159 WP_239052883.1 hypothetical protein -
  MF133_RS15565 (MF133_15565) - 3355636..3357318 (+) 1683 WP_239052884.1 DUF927 domain-containing protein -
  MF133_RS15570 (MF133_15570) - 3357369..3357632 (+) 264 WP_045523228.1 pyocin activator PrtN family protein -
  MF133_RS15575 (MF133_15575) zur 3358211..3358669 (+) 459 WP_039039955.1 zinc uptake transcriptional repressor Zur -
  MF133_RS15580 (MF133_15580) - 3358659..3359120 (+) 462 WP_010673043.1 chemotaxis protein CheX -
  MF133_RS15585 (MF133_15585) - 3359126..3359353 (+) 228 WP_161646839.1 hypothetical protein -
  MF133_RS15590 (MF133_15590) brnQ 3359443..3360705 (-) 1263 WP_050485178.1 branched-chain amino acid transport system II carrier protein -
  MF133_RS15595 (MF133_15595) - 3360941..3361651 (-) 711 WP_049636473.1 methyltransferase -
  MF133_RS15600 (MF133_15600) srmB 3361779..3362999 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  MF133_RS15605 (MF133_15605) yaaA 3363187..3363960 (-) 774 WP_239052885.1 peroxide stress protein YaaA -
  MF133_RS15610 (MF133_15610) pilU 3363993..3365102 (-) 1110 WP_041214461.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MF133_RS15615 (MF133_15615) pilT 3365126..3366160 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  MF133_RS15620 (MF133_15620) - 3366200..3366901 (+) 702 WP_049636471.1 YggS family pyridoxal phosphate-dependent enzyme -
  MF133_RS15625 (MF133_15625) proC 3367025..3367849 (+) 825 WP_239052886.1 pyrroline-5-carboxylate reductase -
  MF133_RS15630 (MF133_15630) - 3367873..3368424 (+) 552 WP_010673053.1 YggT family protein -
  MF133_RS15635 (MF133_15635) yggU 3368424..3368723 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  MF133_RS15640 (MF133_15640) - 3368742..3369162 (+) 421 Protein_3022 DUF4426 domain-containing protein -
  MF133_RS15645 (MF133_15645) - 3369238..3370070 (-) 833 Protein_3023 CPBP family intramembrane glutamic endopeptidase -
  MF133_RS15650 (MF133_15650) - 3370232..3370522 (-) 291 WP_039039963.1 hypothetical protein -
  MF133_RS15655 (MF133_15655) - 3370572..3370946 (-) 375 WP_039039964.1 YacL family protein -
  MF133_RS15660 (MF133_15660) - 3371153..3372268 (+) 1116 WP_039039965.1 sugar diacid recognition domain-containing protein -
  MF133_RS15665 (MF133_15665) - 3372388..3373650 (+) 1263 WP_039039966.1 GntP family permease -
  MF133_RS15670 (MF133_15670) - 3373660..3374793 (+) 1134 WP_239052887.1 glycerate kinase -
  MF133_RS15675 (MF133_15675) - 3375061..3375625 (+) 565 Protein_3029 NnrU family protein -
  MF133_RS15680 (MF133_15680) - 3375685..3376221 (+) 537 WP_010673063.1 glutathione peroxidase -
  MF133_RS15685 (MF133_15685) - 3376304..3377239 (+) 936 WP_010673064.1 LysR family transcriptional regulator -
  MF133_RS15690 (MF133_15690) - 3377422..3378619 (+) 1198 Protein_3032 NupC/NupG family nucleoside CNT transporter -
  MF133_RS15695 (MF133_15695) - 3379230..3379724 (-) 495 WP_239052888.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  MF133_RS15700 (MF133_15700) arfB 3379751..3380167 (-) 417 WP_010673067.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  MF133_RS15705 (MF133_15705) glnK 3380369..3380707 (+) 339 WP_005332325.1 P-II family nitrogen regulator -
  MF133_RS15710 (MF133_15710) - 3380936..3381943 (+) 1008 WP_239052889.1 Fe(3+) ABC transporter substrate-binding protein -
  MF133_RS15715 (MF133_15715) - 3381983..3383608 (+) 1626 WP_151027177.1 iron ABC transporter permease -
  MF133_RS15720 (MF133_15720) - 3383649..3384683 (+) 1035 WP_104451894.1 ABC transporter ATP-binding protein -
  MF133_RS15725 (MF133_15725) - 3384773..3385678 (+) 906 WP_042864452.1 Dyp-type peroxidase -
  MF133_RS15730 (MF133_15730) argR 3385758..3386228 (-) 471 WP_005308670.1 transcriptional regulator ArgR -
  MF133_RS15735 (MF133_15735) - 3386429..3387169 (+) 741 WP_041214472.1 ATP-binding cassette domain-containing protein -
  MF133_RS15740 (MF133_15740) - 3387194..3387931 (+) 738 WP_010673073.1 arginine ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38189.90 Da        Isoelectric Point: 6.7436

>NTDB_id=656173 MF133_RS15615 WP_010673050.1 3365126..3366160(-) (pilT) [Aeromonas caviae strain 211703]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLDDLGAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVASLDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=656173 MF133_RS15615 WP_010673050.1 3365126..3366160(-) (pilT) [Aeromonas caviae strain 211703]
ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTCCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGGAAGTCCATGCTCTCATTTACGACA
TCATGAATGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAAGTACCCGGCATGGCACGC
TTTCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCCGTGTTTCGTACCATCCCGAGCACCGTTCTGACCCTGGA
CGATCTGGGGGCGCCGGAGATATTTCGCAAGATAGCGGAGTACCCTCGCGGCCTGGTGCTGGTGACTGGGCCCACCGGCT
CGGGCAAATCCACCACCCTGGCCGCCATGGTCGATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGATATCATCCTGGTAGGTGAAATGCGGGACCTCGAAACCATCCGCCTCG
CCATGACCGCCGCCGAAACCGGTCACCTGGTGTTTGGCACCCTGCACACCTCCTCGGCTGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGATCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTGGCCCAGCTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAGCAGCTGGTCAACCGGGGGGTCGTCGCCTCCCTCGATGCCAAGGCCAAGGCGGTCGATCCCAATACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081LS93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Pseudomonas stutzeri DSM 10701

78.299

99.128

0.776
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Pseudomonas aeruginosa PAK

76.833

99.128

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

71.471

98.837

0.706
  pilT Legionella pneumophila strain ERS1305867

71.471

98.837

0.706
  pilT Neisseria meningitidis 8013

69.027

98.547

0.68
  pilT Neisseria gonorrhoeae MS11

68.732

98.547

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.924

99.419

0.526
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407