Detailed information
Bioinformatically predicted
Overview
| Name | pilU | Type | Machinery gene |
| Locus tag | MF133_RS15610 | Genome accession | NZ_CP092181 |
| Coordinates | 3363993..3365102 (-) | Length | 369 a.a. |
| NCBI ID | WP_041214461.1 | Uniprot ID | A0A839D3X0 |
| Organism | Aeromonas caviae strain 211703 | ||
| Function | type IV pilus retraction (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 3321797..3388507 | 3363993..3365102 | within | 0 |
Gene organization within MGE regions
Location: 3321797..3388507
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MF133_RS15415 (MF133_15415) | rlmD | 3322559..3323911 (+) | 1353 | WP_043152994.1 | 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD | - |
| MF133_RS15420 (MF133_15420) | - | 3324099..3324608 (+) | 510 | WP_239054223.1 | GNAT family N-acetyltransferase | - |
| MF133_RS15425 (MF133_15425) | - | 3324690..3324860 (+) | 171 | WP_167418745.1 | DUF5363 family protein | - |
| MF133_RS15430 (MF133_15430) | tppF | 3324829..3325443 (-) | 615 | WP_029313748.1 | type IVa pilus pseudopilin TppF | - |
| MF133_RS15435 (MF133_15435) | - | 3325484..3326272 (+) | 789 | WP_041214451.1 | TatD family hydrolase | - |
| MF133_RS15440 (MF133_15440) | - | 3326486..3327760 (+) | 1275 | WP_201871602.1 | NupC/NupG family nucleoside CNT transporter | - |
| MF133_RS15445 (MF133_15445) | - | 3327884..3328054 (+) | 171 | WP_010673027.1 | DUF1427 family protein | - |
| MF133_RS15450 (MF133_15450) | deoC | 3328442..3329215 (+) | 774 | WP_039039946.1 | deoxyribose-phosphate aldolase | - |
| MF133_RS15455 (MF133_15455) | deoA | 3329298..3330629 (+) | 1332 | WP_239052873.1 | thymidine phosphorylase | - |
| MF133_RS15460 (MF133_15460) | - | 3330644..3331852 (+) | 1209 | WP_039039948.1 | phosphopentomutase | - |
| MF133_RS15465 (MF133_15465) | deoD | 3331986..3332702 (+) | 717 | WP_005341325.1 | purine-nucleoside phosphorylase | - |
| MF133_RS15470 (MF133_15470) | - | 3332767..3335013 (-) | 2247 | WP_239052874.1 | PAS domain S-box protein | - |
| MF133_RS15475 (MF133_15475) | - | 3335079..3335687 (-) | 609 | WP_010673032.1 | AhpA/YtjB family protein | - |
| MF133_RS15480 (MF133_15480) | serB | 3335881..3336885 (+) | 1005 | WP_239052875.1 | phosphoserine phosphatase SerB | - |
| MF133_RS15485 (MF133_15485) | - | 3336914..3337819 (+) | 906 | WP_043153013.1 | alpha/beta fold hydrolase | - |
| MF133_RS15490 (MF133_15490) | - | 3337876..3340251 (-) | 2376 | WP_239052876.1 | PilZ domain-containing protein | - |
| MF133_RS15495 (MF133_15495) | radA | 3340352..3341716 (+) | 1365 | WP_010673036.1 | DNA repair protein RadA | - |
| MF133_RS15500 (MF133_15500) | - | 3341770..3342156 (-) | 387 | WP_029313755.1 | PilZ domain-containing protein | - |
| MF133_RS15505 (MF133_15505) | - | 3342259..3342741 (-) | 483 | WP_029313757.1 | Rsd/AlgQ family anti-sigma factor | - |
| MF133_RS15510 (MF133_15510) | hupA | 3343080..3343352 (+) | 273 | WP_005305063.1 | nucleoid-associated protein HU-alpha | - |
| MF133_RS15515 (MF133_15515) | - | 3343466..3343891 (+) | 426 | WP_029313758.1 | hypothetical protein | - |
| MF133_RS15520 (MF133_15520) | - | 3343974..3344123 (-) | 150 | WP_080944170.1 | hypothetical protein | - |
| MF133_RS15525 (MF133_15525) | dusA | 3344126..3345082 (-) | 957 | WP_239052877.1 | tRNA dihydrouridine(20/20a) synthase DusA | - |
| MF133_RS15530 (MF133_15530) | - | 3345267..3346367 (+) | 1101 | WP_239052878.1 | site-specific integrase | - |
| MF133_RS15535 (MF133_15535) | - | 3346527..3347567 (+) | 1041 | Protein_3001 | IS481 family transposase | - |
| MF133_RS15540 (MF133_15540) | - | 3347717..3351787 (-) | 4071 | WP_239052879.1 | hypothetical protein | - |
| MF133_RS15545 (MF133_15545) | mobI | 3352348..3352923 (+) | 576 | WP_239052880.1 | conjugative transfer protein MobI(A/C) | - |
| MF133_RS15550 (MF133_15550) | - | 3353579..3354436 (+) | 858 | WP_239052881.1 | hypothetical protein | - |
| MF133_RS15555 (MF133_15555) | - | 3354711..3355058 (+) | 348 | WP_239052882.1 | hypothetical protein | - |
| MF133_RS15560 (MF133_15560) | - | 3355236..3355394 (-) | 159 | WP_239052883.1 | hypothetical protein | - |
| MF133_RS15565 (MF133_15565) | - | 3355636..3357318 (+) | 1683 | WP_239052884.1 | DUF927 domain-containing protein | - |
| MF133_RS15570 (MF133_15570) | - | 3357369..3357632 (+) | 264 | WP_045523228.1 | pyocin activator PrtN family protein | - |
| MF133_RS15575 (MF133_15575) | zur | 3358211..3358669 (+) | 459 | WP_039039955.1 | zinc uptake transcriptional repressor Zur | - |
| MF133_RS15580 (MF133_15580) | - | 3358659..3359120 (+) | 462 | WP_010673043.1 | chemotaxis protein CheX | - |
| MF133_RS15585 (MF133_15585) | - | 3359126..3359353 (+) | 228 | WP_161646839.1 | hypothetical protein | - |
| MF133_RS15590 (MF133_15590) | brnQ | 3359443..3360705 (-) | 1263 | WP_050485178.1 | branched-chain amino acid transport system II carrier protein | - |
| MF133_RS15595 (MF133_15595) | - | 3360941..3361651 (-) | 711 | WP_049636473.1 | methyltransferase | - |
| MF133_RS15600 (MF133_15600) | srmB | 3361779..3362999 (+) | 1221 | WP_041210934.1 | ATP-dependent RNA helicase SrmB | - |
| MF133_RS15605 (MF133_15605) | yaaA | 3363187..3363960 (-) | 774 | WP_239052885.1 | peroxide stress protein YaaA | - |
| MF133_RS15610 (MF133_15610) | pilU | 3363993..3365102 (-) | 1110 | WP_041214461.1 | PilT/PilU family type 4a pilus ATPase | Machinery gene |
| MF133_RS15615 (MF133_15615) | pilT | 3365126..3366160 (-) | 1035 | WP_010673050.1 | type IVa pilus ATPase TapT | Machinery gene |
| MF133_RS15620 (MF133_15620) | - | 3366200..3366901 (+) | 702 | WP_049636471.1 | YggS family pyridoxal phosphate-dependent enzyme | - |
| MF133_RS15625 (MF133_15625) | proC | 3367025..3367849 (+) | 825 | WP_239052886.1 | pyrroline-5-carboxylate reductase | - |
| MF133_RS15630 (MF133_15630) | - | 3367873..3368424 (+) | 552 | WP_010673053.1 | YggT family protein | - |
| MF133_RS15635 (MF133_15635) | yggU | 3368424..3368723 (+) | 300 | WP_010673054.1 | DUF167 family protein YggU | - |
| MF133_RS15640 (MF133_15640) | - | 3368742..3369162 (+) | 421 | Protein_3022 | DUF4426 domain-containing protein | - |
| MF133_RS15645 (MF133_15645) | - | 3369238..3370070 (-) | 833 | Protein_3023 | CPBP family intramembrane glutamic endopeptidase | - |
| MF133_RS15650 (MF133_15650) | - | 3370232..3370522 (-) | 291 | WP_039039963.1 | hypothetical protein | - |
| MF133_RS15655 (MF133_15655) | - | 3370572..3370946 (-) | 375 | WP_039039964.1 | YacL family protein | - |
| MF133_RS15660 (MF133_15660) | - | 3371153..3372268 (+) | 1116 | WP_039039965.1 | sugar diacid recognition domain-containing protein | - |
| MF133_RS15665 (MF133_15665) | - | 3372388..3373650 (+) | 1263 | WP_039039966.1 | GntP family permease | - |
| MF133_RS15670 (MF133_15670) | - | 3373660..3374793 (+) | 1134 | WP_239052887.1 | glycerate kinase | - |
| MF133_RS15675 (MF133_15675) | - | 3375061..3375625 (+) | 565 | Protein_3029 | NnrU family protein | - |
| MF133_RS15680 (MF133_15680) | - | 3375685..3376221 (+) | 537 | WP_010673063.1 | glutathione peroxidase | - |
| MF133_RS15685 (MF133_15685) | - | 3376304..3377239 (+) | 936 | WP_010673064.1 | LysR family transcriptional regulator | - |
| MF133_RS15690 (MF133_15690) | - | 3377422..3378619 (+) | 1198 | Protein_3032 | NupC/NupG family nucleoside CNT transporter | - |
| MF133_RS15695 (MF133_15695) | - | 3379230..3379724 (-) | 495 | WP_239052888.1 | methylated-DNA--[protein]-cysteine S-methyltransferase | - |
| MF133_RS15700 (MF133_15700) | arfB | 3379751..3380167 (-) | 417 | WP_010673067.1 | alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB | - |
| MF133_RS15705 (MF133_15705) | glnK | 3380369..3380707 (+) | 339 | WP_005332325.1 | P-II family nitrogen regulator | - |
| MF133_RS15710 (MF133_15710) | - | 3380936..3381943 (+) | 1008 | WP_239052889.1 | Fe(3+) ABC transporter substrate-binding protein | - |
| MF133_RS15715 (MF133_15715) | - | 3381983..3383608 (+) | 1626 | WP_151027177.1 | iron ABC transporter permease | - |
| MF133_RS15720 (MF133_15720) | - | 3383649..3384683 (+) | 1035 | WP_104451894.1 | ABC transporter ATP-binding protein | - |
| MF133_RS15725 (MF133_15725) | - | 3384773..3385678 (+) | 906 | WP_042864452.1 | Dyp-type peroxidase | - |
| MF133_RS15730 (MF133_15730) | argR | 3385758..3386228 (-) | 471 | WP_005308670.1 | transcriptional regulator ArgR | - |
| MF133_RS15735 (MF133_15735) | - | 3386429..3387169 (+) | 741 | WP_041214472.1 | ATP-binding cassette domain-containing protein | - |
| MF133_RS15740 (MF133_15740) | - | 3387194..3387931 (+) | 738 | WP_010673073.1 | arginine ABC transporter substrate-binding protein | - |
Sequence
Protein
Download Length: 369 a.a. Molecular weight: 40856.99 Da Isoelectric Point: 6.3155
>NTDB_id=656172 MF133_RS15610 WP_041214461.1 3363993..3365102(-) (pilU) [Aeromonas caviae strain 211703]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFGLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFGLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE
Nucleotide
Download Length: 1110 bp
>NTDB_id=656172 MF133_RS15610 WP_041214461.1 3363993..3365102(-) (pilU) [Aeromonas caviae strain 211703]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGAGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCTCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCACAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGGCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGAGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCTCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCACAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGGCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilU | Vibrio cholerae strain A1552 |
60.598 |
99.729 |
0.604 |
| pilU | Pseudomonas stutzeri DSM 10701 |
58.974 |
95.122 |
0.561 |
| pilU | Acinetobacter baylyi ADP1 |
57.79 |
95.664 |
0.553 |
| pilT | Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 |
43.155 |
91.057 |
0.393 |
| pilT | Legionella pneumophila strain Lp02 |
40.816 |
92.954 |
0.379 |
| pilT | Legionella pneumophila strain ERS1305867 |
40.816 |
92.954 |
0.379 |