Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L3Q72_RS02035 Genome accession   NZ_CP092106
Coordinates   412731..413837 (+) Length   368 a.a.
NCBI ID   WP_275131017.1    Uniprot ID   -
Organism   Vibrio sp. JC009     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 407731..418837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3Q72_RS02005 (L3Q72_02000) - 408458..409057 (-) 600 WP_275131011.1 XTP/dITP diphosphatase -
  L3Q72_RS02010 (L3Q72_02005) yggU 409084..409371 (-) 288 WP_275131012.1 DUF167 family protein YggU -
  L3Q72_RS02015 (L3Q72_02010) - 409371..409928 (-) 558 WP_275131013.1 YggT family protein -
  L3Q72_RS02020 (L3Q72_02015) proC 409947..410765 (-) 819 WP_275131014.1 pyrroline-5-carboxylate reductase -
  L3Q72_RS02025 (L3Q72_02020) - 410790..411500 (-) 711 WP_275131015.1 YggS family pyridoxal phosphate-dependent enzyme -
  L3Q72_RS02030 (L3Q72_02025) pilT 411525..412568 (+) 1044 WP_275131016.1 type IV pilus twitching motility protein PilT Machinery gene
  L3Q72_RS02035 (L3Q72_02030) pilU 412731..413837 (+) 1107 WP_275131017.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L3Q72_RS02040 (L3Q72_02035) ruvX 413854..414282 (-) 429 WP_275131018.1 Holliday junction resolvase RuvX -
  L3Q72_RS02045 (L3Q72_02040) - 414405..414968 (-) 564 WP_275131019.1 YqgE/AlgH family protein -
  L3Q72_RS02050 (L3Q72_02045) gshB 415010..415957 (-) 948 WP_275131020.1 glutathione synthase -
  L3Q72_RS02055 (L3Q72_02050) rsmE 415971..416702 (-) 732 WP_275131021.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L3Q72_RS02060 (L3Q72_02055) - 416793..417488 (-) 696 WP_275131022.1 endonuclease -
  L3Q72_RS02065 (L3Q72_02060) - 417566..418072 (-) 507 WP_275131023.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41044.90 Da        Isoelectric Point: 5.2134

>NTDB_id=655895 L3Q72_RS02035 WP_275131017.1 412731..413837(+) (pilU) [Vibrio sp. JC009]
MQLDQILESMLENKASDLYITVDAPCLLRVHGELTGVGEKLNEEGVFQFLDTIMDEQRKQDYLASKEANFAIVRNSGRFR
VSAFFQRELPGAVIRRIETVIPTFEELKLPEVLKELATAKRGLVLVVGATGSGKSTTMAAMTGYRNDNRSGHILTVEDPI
EFVHEHKKCLVTQREVGLDTESFEVALKNSLRQAPDMILIGEIRSRETMEFAMNFAETGHLCMATLHANNANQALERILN
LVPKEGRDQFLFDLSNNLKGVIAQQLIKDKNGNARHGCFELLLNTPRIADLIRRGDLHELKATMARSTESGMKTFDQSLY
ELVIEDKIAEDDALHSADSANDLRMMLKAKRGGNFGSGAMDGVSIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=655895 L3Q72_RS02035 WP_275131017.1 412731..413837(+) (pilU) [Vibrio sp. JC009]
ATGCAGTTGGATCAGATTCTGGAATCGATGCTGGAAAACAAAGCATCGGATCTTTATATCACGGTGGATGCACCCTGTTT
GTTGCGGGTGCATGGTGAGCTTACCGGCGTAGGTGAAAAACTGAATGAAGAGGGCGTGTTTCAGTTTCTTGATACTATTA
TGGATGAGCAGCGTAAGCAGGATTATCTGGCGAGTAAGGAGGCCAACTTTGCCATTGTCAGAAATAGTGGCCGTTTCCGT
GTCAGTGCTTTTTTTCAGAGGGAGTTGCCGGGGGCCGTTATCCGCCGGATTGAAACTGTGATTCCGACCTTTGAAGAGCT
GAAGCTTCCCGAAGTACTGAAAGAGCTGGCAACAGCTAAACGCGGCCTGGTGCTGGTGGTAGGGGCGACGGGTTCCGGTA
AATCAACTACCATGGCGGCAATGACGGGATACAGAAATGATAACCGGTCAGGTCATATTCTTACCGTGGAAGATCCCATT
GAGTTTGTCCACGAACATAAGAAGTGTCTGGTGACACAAAGGGAAGTGGGATTAGATACGGAGAGCTTTGAAGTGGCGCT
GAAAAACTCCCTTCGTCAGGCTCCGGATATGATTCTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAGTTTGCCATGA
ACTTTGCGGAAACCGGGCATCTGTGTATGGCGACTTTGCACGCCAATAATGCCAATCAGGCGCTGGAGCGAATTCTTAAC
CTTGTTCCGAAAGAGGGTAGGGATCAGTTTTTGTTTGATCTTTCCAATAATCTGAAGGGCGTAATAGCGCAGCAGTTGAT
CAAAGACAAAAACGGCAATGCCCGCCATGGCTGCTTTGAGTTATTGCTAAATACCCCAAGGATCGCCGATTTGATCCGGC
GAGGCGATCTGCATGAGCTGAAAGCGACCATGGCAAGATCAACCGAAAGCGGTATGAAGACTTTTGATCAGTCTCTTTAT
GAATTGGTGATCGAGGATAAGATCGCTGAAGATGATGCTCTGCACAGCGCAGATTCTGCCAATGATCTGAGAATGATGCT
AAAAGCGAAGCGCGGTGGCAATTTTGGTTCTGGCGCTATGGATGGGGTTAGTATTGATATGGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(103-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

77.989

100

0.78
  pilU Pseudomonas stutzeri DSM 10701

55.256

100

0.557
  pilU Acinetobacter baylyi ADP1

52.381

97.011

0.508
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.846

88.315

0.37
  pilT Vibrio cholerae strain A1552

41.846

88.315

0.37
  pilT Neisseria meningitidis 8013

39.13

93.75

0.367
  pilT Neisseria gonorrhoeae MS11

39.13

93.75

0.367
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.528

92.12

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.528

92.12

0.364