Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L3Q72_RS02030 Genome accession   NZ_CP092106
Coordinates   411525..412568 (+) Length   347 a.a.
NCBI ID   WP_275131016.1    Uniprot ID   -
Organism   Vibrio sp. JC009     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406525..417568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3Q72_RS02000 (L3Q72_01995) hemW 407307..408458 (-) 1152 WP_275131010.1 radical SAM family heme chaperone HemW -
  L3Q72_RS02005 (L3Q72_02000) - 408458..409057 (-) 600 WP_275131011.1 XTP/dITP diphosphatase -
  L3Q72_RS02010 (L3Q72_02005) yggU 409084..409371 (-) 288 WP_275131012.1 DUF167 family protein YggU -
  L3Q72_RS02015 (L3Q72_02010) - 409371..409928 (-) 558 WP_275131013.1 YggT family protein -
  L3Q72_RS02020 (L3Q72_02015) proC 409947..410765 (-) 819 WP_275131014.1 pyrroline-5-carboxylate reductase -
  L3Q72_RS02025 (L3Q72_02020) - 410790..411500 (-) 711 WP_275131015.1 YggS family pyridoxal phosphate-dependent enzyme -
  L3Q72_RS02030 (L3Q72_02025) pilT 411525..412568 (+) 1044 WP_275131016.1 type IV pilus twitching motility protein PilT Machinery gene
  L3Q72_RS02035 (L3Q72_02030) pilU 412731..413837 (+) 1107 WP_275131017.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L3Q72_RS02040 (L3Q72_02035) ruvX 413854..414282 (-) 429 WP_275131018.1 Holliday junction resolvase RuvX -
  L3Q72_RS02045 (L3Q72_02040) - 414405..414968 (-) 564 WP_275131019.1 YqgE/AlgH family protein -
  L3Q72_RS02050 (L3Q72_02045) gshB 415010..415957 (-) 948 WP_275131020.1 glutathione synthase -
  L3Q72_RS02055 (L3Q72_02050) rsmE 415971..416702 (-) 732 WP_275131021.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L3Q72_RS02060 (L3Q72_02055) - 416793..417488 (-) 696 WP_275131022.1 endonuclease -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38495.02 Da        Isoelectric Point: 6.4424

>NTDB_id=655894 L3Q72_RS02030 WP_275131016.1 411525..412568(+) (pilT) [Vibrio sp. JC009]
MDIAELLQFSVKHNASDLHLSAGVPPMIRIDGDVRKLGMPAFSHSDVHRLVFDIMNDAQRSEFEEKLETDFSFELPDVGR
FRVNAFNQSRGCAAVFRTIPTRIPTLDELSAPEIFSEIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNQHHNKHILTIED
PIEFVHQNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKNMVRSMLSESLRAVIAQKLLKRVGGGRVACHEVMIANNAIRNLIREDKVAQMLSIIQTGAASGMKTMEQSA
KQLLARGEVEQEEVNKKIEIVQMSSTF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=655894 L3Q72_RS02030 WP_275131016.1 411525..412568(+) (pilT) [Vibrio sp. JC009]
ATGGATATCGCTGAGTTACTGCAATTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCAGGTGTTCCTCCAAT
GATAAGGATAGATGGTGATGTAAGAAAGCTTGGAATGCCGGCATTTTCTCATTCTGATGTTCACAGACTGGTTTTTGACA
TTATGAATGATGCGCAAAGAAGCGAATTTGAAGAAAAGCTGGAGACGGATTTTTCCTTTGAGTTGCCGGATGTGGGGCGC
TTTAGGGTGAATGCCTTTAATCAGTCCAGAGGGTGTGCGGCTGTTTTTCGTACCATCCCGACAAGAATTCCGACACTGGA
TGAGCTTTCCGCTCCTGAGATTTTTTCTGAGATAGCAAACTATGAAAAAGGTCTGGTTCTGGTGACTGGCCCAACGGGCT
CTGGTAAGTCTACGACGCTGGCGGCAATGGTTGACTATGTTAACCAGCACCATAATAAGCATATCCTGACCATTGAAGAT
CCGATTGAGTTTGTTCATCAGAATAATAAGTGTCTGATTAACCAGCGGGAAGTGCACAGGGATACCCATAGCTTTAAAGC
GGCTCTGCGTTCTGCTTTACGTGAAGACCCGGATGTGATTCTGGTGGGTGAGCTTCGCGATCAGGAAACCATTAGTCTGG
CATTAACAGCAGCGGAAACCGGGCATCTGGTATTTGGTACTCTGCACACCAGTTCTGCGGCTAAAACCATTGACCGTATT
ATTGATGTGTTCCCGGGCTCTGAGAAAAATATGGTTCGTTCAATGCTGTCTGAGTCGCTTCGCGCGGTTATTGCTCAGAA
GCTGCTAAAGCGTGTTGGCGGGGGCAGGGTGGCCTGTCATGAGGTGATGATTGCTAACAACGCTATCCGTAACCTTATCC
GCGAAGATAAAGTAGCTCAGATGTTGTCTATTATTCAGACGGGGGCAGCTTCAGGTATGAAAACCATGGAGCAGAGTGCG
AAGCAGCTTCTGGCGCGGGGAGAGGTTGAGCAGGAAGAAGTAAATAAGAAAATTGAGATAGTGCAAATGAGTTCTACTTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.235

97.983

0.865
  pilT Vibrio cholerae strain A1552

88.235

97.983

0.865
  pilT Acinetobacter baumannii D1279779

73.394

94.236

0.692
  pilT Acinetobacter baumannii strain A118

73.394

94.236

0.692
  pilT Acinetobacter nosocomialis M2

73.089

94.236

0.689
  pilT Acinetobacter baylyi ADP1

73.089

94.236

0.689
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.118

0.674
  pilT Pseudomonas aeruginosa PAK

68.235

97.983

0.669
  pilT Legionella pneumophila strain Lp02

69.325

93.948

0.651
  pilT Legionella pneumophila strain ERS1305867

69.325

93.948

0.651
  pilT Neisseria meningitidis 8013

63.824

97.983

0.625
  pilT Neisseria gonorrhoeae MS11

63.529

97.983

0.622
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.211

94.236

0.501
  pilU Vibrio cholerae strain A1552

42.515

96.254

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.317

96.254

0.398
  pilU Acinetobacter baylyi ADP1

40.81

92.507

0.378