Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L8M16_RS18310 Genome accession   NZ_CP091977
Coordinates   3832205..3833248 (+) Length   347 a.a.
NCBI ID   WP_011236808.1    Uniprot ID   Q5P6H8
Organism   Aromatoleum aromaticum strain pCyN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3827205..3838248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8M16_RS18280 - 3827357..3828328 (+) 972 WP_011236802.1 aspartate carbamoyltransferase catalytic subunit -
  L8M16_RS18285 - 3828329..3829606 (+) 1278 WP_168954033.1 dihydroorotase -
  L8M16_RS18290 - 3829695..3829988 (-) 294 WP_011236804.1 DUF167 family protein -
  L8M16_RS18295 - 3829994..3830572 (-) 579 WP_168954032.1 YggT family protein -
  L8M16_RS18300 proC 3830579..3831391 (-) 813 WP_168954043.1 pyrroline-5-carboxylate reductase -
  L8M16_RS18305 - 3831400..3832101 (-) 702 WP_168954031.1 YggS family pyridoxal phosphate-dependent enzyme -
  L8M16_RS18310 pilT 3832205..3833248 (+) 1044 WP_011236808.1 type IV pilus twitching motility protein PilT Machinery gene
  L8M16_RS18315 pilU 3833384..3834517 (+) 1134 WP_168954030.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L8M16_RS18320 - 3834573..3835733 (-) 1161 WP_168954029.1 class I SAM-dependent RNA methyltransferase -
  L8M16_RS18325 - 3835839..3836258 (-) 420 WP_168954028.1 CopD family protein -
  L8M16_RS18330 - 3836258..3837145 (-) 888 WP_168954027.1 SDR family oxidoreductase -
  L8M16_RS18335 - 3837167..3838192 (+) 1026 WP_168954026.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38393.98 Da        Isoelectric Point: 6.7747

>NTDB_id=654831 L8M16_RS18310 WP_011236808.1 3832205..3833248(+) (pilT) [Aromatoleum aromaticum strain pCyN1]
MDITELLAFAVKNKASDLHLSSGLPPMIRVHGDVRRINLPPMEHKDVHSMVYDIMNDGQRKQFEEVLECDFSFAVPNLAR
FRVNAFNQNRGASAVFRTIPTKVLSLEELDAPKIFKEIANQPRGIVLVTGPTGSGKSTTLAAMVDYVNENDYGHILTIED
PIEFVHESKRCLINQREVARDTLSFNNALRSALREDPDVILVGELRDLETIRLALTGAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKDMVRAMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQAVGMQTLDQN
LADLVRRNVVSVGEARLRAQNKDNFAG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=654831 L8M16_RS18310 WP_011236808.1 3832205..3833248(+) (pilT) [Aromatoleum aromaticum strain pCyN1]
ATGGACATCACCGAACTGCTTGCCTTCGCGGTCAAGAACAAGGCATCCGACCTGCATCTGTCATCCGGACTGCCGCCGAT
GATCCGGGTGCATGGCGACGTACGGCGCATCAACCTGCCGCCGATGGAGCACAAGGACGTGCACTCGATGGTGTATGACA
TCATGAACGACGGCCAGCGCAAGCAGTTCGAGGAAGTGCTCGAATGCGACTTCTCGTTCGCGGTGCCGAACCTCGCGCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCTCGGCGGTGTTCCGGACGATTCCGACGAAAGTGCTGAGCCTCGA
GGAACTGGACGCGCCGAAGATCTTCAAGGAGATCGCGAACCAGCCGCGCGGCATCGTATTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACGACCCTCGCCGCGATGGTCGATTACGTCAATGAGAACGACTACGGCCACATCCTGACGATCGAGGAT
CCGATCGAATTCGTCCATGAATCGAAACGCTGCCTGATCAACCAGCGCGAAGTCGCCCGCGACACGCTGTCGTTCAACAA
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACGTGATCCTCGTCGGCGAGCTGCGCGACCTCGAGACGATCCGCCTCG
CGCTGACCGGCGCCGAGACCGGCCACCTCGTGTTCGGCACGCTGCACACTTCGTCGGCGGCGAAGACCGTCGACCGGATC
GTCGACGTCTTTCCGGCCGCCGAGAAGGACATGGTGCGCGCGATGCTGTCGGAGTCGCTGCGCGCGGTGATCTCGCAGAC
GCTCTTGAAGACCAAGGACGGCCAGGGCCGCGTCGCGGCGCACGAGATCATGATCGGCACTCCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGGCGGTGGGCATGCAGACGCTCGACCAGAAC
CTCGCCGACCTCGTGCGGCGCAACGTCGTGTCGGTCGGCGAAGCCAGGCTGCGCGCGCAGAACAAGGACAACTTCGCCGG
GTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5P6H8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.971

99.424

0.775
  pilT Acinetobacter baumannii D1279779

77.971

99.424

0.775
  pilT Acinetobacter baumannii strain A118

77.971

99.424

0.775
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

72.754

99.424

0.723
  pilT Legionella pneumophila strain ERS1305867

72.754

99.424

0.723
  pilT Neisseria meningitidis 8013

71.965

99.712

0.718
  pilT Neisseria gonorrhoeae MS11

71.676

99.712

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.833

96.83

0.686
  pilT Vibrio cholerae strain A1552

70.833

96.83

0.686
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.601

99.712

0.524
  pilU Acinetobacter baylyi ADP1

42.241

100

0.424
  pilU Pseudomonas stutzeri DSM 10701

41.159

99.424

0.409
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403