Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L8M16_RS18315 Genome accession   NZ_CP091977
Coordinates   3833384..3834517 (+) Length   377 a.a.
NCBI ID   WP_168954030.1    Uniprot ID   -
Organism   Aromatoleum aromaticum strain pCyN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3828384..3839517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8M16_RS18290 - 3829695..3829988 (-) 294 WP_011236804.1 DUF167 family protein -
  L8M16_RS18295 - 3829994..3830572 (-) 579 WP_168954032.1 YggT family protein -
  L8M16_RS18300 proC 3830579..3831391 (-) 813 WP_168954043.1 pyrroline-5-carboxylate reductase -
  L8M16_RS18305 - 3831400..3832101 (-) 702 WP_168954031.1 YggS family pyridoxal phosphate-dependent enzyme -
  L8M16_RS18310 pilT 3832205..3833248 (+) 1044 WP_011236808.1 type IV pilus twitching motility protein PilT Machinery gene
  L8M16_RS18315 pilU 3833384..3834517 (+) 1134 WP_168954030.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L8M16_RS18320 - 3834573..3835733 (-) 1161 WP_168954029.1 class I SAM-dependent RNA methyltransferase -
  L8M16_RS18325 - 3835839..3836258 (-) 420 WP_168954028.1 CopD family protein -
  L8M16_RS18330 - 3836258..3837145 (-) 888 WP_168954027.1 SDR family oxidoreductase -
  L8M16_RS18335 - 3837167..3838192 (+) 1026 WP_168954026.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  L8M16_RS18340 - 3838197..3838370 (+) 174 Protein_3608 regulator -
  L8M16_RS18345 - 3838524..3838832 (-) 309 WP_041645820.1 c-type cytochrome -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42247.53 Da        Isoelectric Point: 6.4340

>NTDB_id=654832 L8M16_RS18315 WP_168954030.1 3833384..3834517(+) (pilU) [Aromatoleum aromaticum strain pCyN1]
MERDQALKFMHDLLRLMVQKNGSDLFITAGFPPAIKVDGRVTPQSNQTLTAQHTTELARAIMNDRQAAEFESTKECNFAI
SPPGVGRFRANAFIQQGKVGLVLRTIAQTIPDFDELGMPAVLREIAMAKRGLVIFVGGTGTGKTTSLAAMVDFRNEHSYG
HIITVEDPIEYVHQHKNCIVTQREIGIDTDNWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRPQLLMDLSLNLRALISQRLLPLKERKGRVPAVEVLLNSPLIADLIFKGEVPGLKEVMKRSRELG
MQTFDQSLFDLYEEERISYEDALRNADSVNDLRLQIKLHSKHGDKDLVSGIQHLGIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=654832 L8M16_RS18315 WP_168954030.1 3833384..3834517(+) (pilU) [Aromatoleum aromaticum strain pCyN1]
ATGGAACGCGATCAGGCCCTGAAGTTCATGCACGACCTGCTGCGGCTGATGGTGCAGAAAAATGGCTCGGACCTTTTCAT
CACCGCGGGCTTTCCGCCAGCGATCAAGGTGGACGGGCGCGTCACGCCGCAATCGAACCAGACGCTGACGGCGCAGCACA
CGACCGAGCTCGCGCGCGCGATCATGAACGACCGCCAGGCGGCCGAGTTCGAGTCGACGAAGGAATGCAACTTCGCGATC
TCGCCGCCGGGCGTCGGGCGCTTCCGCGCCAACGCGTTCATTCAGCAAGGCAAGGTGGGCCTCGTGCTGCGCACGATCGC
GCAGACGATCCCGGACTTCGACGAGCTCGGCATGCCGGCGGTGCTGCGCGAGATCGCGATGGCCAAGCGCGGGCTGGTGA
TTTTCGTCGGTGGCACCGGCACCGGCAAGACGACGTCGCTCGCAGCGATGGTCGACTTCCGCAACGAGCACTCGTACGGC
CACATCATCACGGTCGAGGATCCGATCGAATACGTGCATCAGCACAAGAACTGCATCGTCACGCAGCGCGAGATCGGCAT
CGACACAGACAACTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGGCCCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACGATGGACTACGCGATCGCGTTCGCCGAAACCGGCCACCTGTGCCTCGCGACGCTGCACGCGAACAGCGCG
AACCAGGCAATCGACCGCATCATCAACTTCTTCCCCGAGGATCGCCGTCCGCAGCTCCTGATGGACCTGTCGCTGAACCT
GCGCGCGCTGATCTCGCAGCGCCTGCTGCCGCTCAAGGAGCGCAAGGGCCGCGTGCCGGCCGTCGAAGTGCTGCTGAATT
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGAAGTGCCGGGGCTCAAGGAAGTCATGAAGCGCTCGCGCGAGCTCGGC
ATGCAGACGTTCGACCAGAGCCTGTTCGACCTCTACGAGGAAGAGCGCATCAGCTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCCACAGCAAACACGGCGACAAGGACCTGGTGTCCGGCATCCAGCACC
TCGGGATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.155

96.021

0.597
  pilU Acinetobacter baylyi ADP1

58.217

95.225

0.554
  pilU Vibrio cholerae strain A1552

55

95.491

0.525
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.881

88.859

0.39
  pilT Pseudomonas aeruginosa PAK

42.388

88.859

0.377
  pilT Pseudomonas stutzeri DSM 10701

42.388

88.859

0.377
  pilT Acinetobacter baumannii strain A118

42.598

87.798

0.374
  pilT Acinetobacter nosocomialis M2

42.598

87.798

0.374
  pilT Acinetobacter baumannii D1279779

42.598

87.798

0.374
  pilT Acinetobacter baylyi ADP1

44.062

84.881

0.374
  pilT Legionella pneumophila strain Lp02

42.296

87.798

0.371
  pilT Legionella pneumophila strain ERS1305867

42.296

87.798

0.371