Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L3V77_RS02080 Genome accession   NZ_CP091975
Coordinates   417157..418200 (+) Length   347 a.a.
NCBI ID   WP_275135505.1    Uniprot ID   -
Organism   Vibrio sp. DW001     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412157..423200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3V77_RS02050 (L3V77_02050) hemW 412879..414027 (-) 1149 WP_275136675.1 radical SAM family heme chaperone HemW -
  L3V77_RS02055 (L3V77_02055) - 414052..414651 (-) 600 WP_275135500.1 XTP/dITP diphosphatase -
  L3V77_RS02060 (L3V77_02060) yggU 414690..414977 (-) 288 WP_275135501.1 DUF167 family protein YggU -
  L3V77_RS02065 (L3V77_02065) - 414977..415531 (-) 555 WP_275135502.1 YggT family protein -
  L3V77_RS02070 (L3V77_02070) proC 415573..416391 (-) 819 WP_275135503.1 pyrroline-5-carboxylate reductase -
  L3V77_RS02075 (L3V77_02075) - 416422..417132 (-) 711 WP_275135504.1 YggS family pyridoxal phosphate-dependent enzyme -
  L3V77_RS02080 (L3V77_02080) pilT 417157..418200 (+) 1044 WP_275135505.1 type IV pilus twitching motility protein PilT Machinery gene
  L3V77_RS02085 (L3V77_02085) pilU 418205..419326 (+) 1122 WP_275135506.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L3V77_RS02090 (L3V77_02090) ruvX 419376..419807 (-) 432 WP_275135507.1 Holliday junction resolvase RuvX -
  L3V77_RS02095 (L3V77_02095) - 419868..420431 (-) 564 WP_275135508.1 YqgE/AlgH family protein -
  L3V77_RS02100 (L3V77_02100) gshB 420450..421397 (-) 948 WP_275135509.1 glutathione synthase -
  L3V77_RS02105 (L3V77_02105) rsmE 421432..422163 (-) 732 WP_275135510.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L3V77_RS02110 (L3V77_02110) - 422282..422782 (-) 501 WP_275135511.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38529.37 Da        Isoelectric Point: 6.0507

>NTDB_id=654777 L3V77_RS02080 WP_275135505.1 417157..418200(+) (pilT) [Vibrio sp. DW001]
MDITELLDFSVKHNASDLHLSAGVAPMIRIDGDVRKLGIPPLSHSEVHHLLFEIMTDAQRSEFEEKLEVDFSYEIPQIGR
FRINAFNQRRGCSAVLRTIPMDIPTLEELDAPSIFSEISEMEKGLILVTGPTGSGKSTTLAAMVNHINRNFNKHILTIED
PIEFVHSNIKCLINQREVHKDTHSFEAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLKTVIAQKLLKKIGGGRVACHEIMMGTPAIRNLIREDKVAQMYSIIQTSSSVGMRTMEQSA
KQLVAKNIVDIDEVNKKIEMEVMSFGS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=654777 L3V77_RS02080 WP_275135505.1 417157..418200(+) (pilT) [Vibrio sp. DW001]
ATGGATATCACTGAGCTATTAGATTTTAGTGTAAAACATAATGCCTCAGATCTACATCTTTCTGCTGGTGTTGCCCCCAT
GATACGCATTGATGGCGATGTTAGGAAGCTAGGTATTCCTCCTTTAAGTCATTCTGAGGTGCATCACCTGTTATTTGAAA
TAATGACGGATGCACAAAGAAGTGAATTTGAAGAGAAGCTTGAAGTCGATTTTTCGTATGAAATTCCACAAATAGGCCGA
TTCCGCATCAATGCTTTTAATCAAAGACGTGGGTGTTCAGCTGTTTTGCGCACCATCCCAATGGATATCCCTACGTTAGA
AGAGTTAGATGCACCAAGTATATTTAGTGAAATTTCCGAAATGGAAAAAGGCTTGATATTGGTTACTGGGCCAACGGGTT
CTGGGAAGTCAACAACGTTAGCGGCAATGGTTAATCATATCAATCGTAACTTTAATAAGCATATATTAACCATCGAAGAT
CCAATTGAATTTGTGCATTCAAATATTAAGTGTTTGATTAATCAACGTGAGGTGCATAAAGACACCCATAGCTTTGAGGC
TGCGCTAAGGTCGGCACTGCGTGAAGACCCAGACGTCATTCTTGTTGGTGAATTAAGGGACCAAGAAACCATTAGTTTAG
CATTGACGGCTGCGGAAACGGGTCATTTGGTATTTGGTACTCTGCATACAAGCTCAGCGGCGAAAACGATTGATCGTATT
ATTGATGTTTTCCCAGGAAGTGACAAATCGATGGTTCGGTCAATGTTGTCTGAATCTCTTAAGACCGTTATTGCACAAAA
ATTATTGAAAAAAATTGGTGGTGGCCGAGTTGCATGTCACGAAATCATGATGGGCACACCCGCAATCCGAAATCTTATCC
GTGAAGATAAAGTTGCCCAAATGTATTCCATAATTCAAACGAGCTCTTCCGTTGGCATGAGAACAATGGAACAGAGTGCG
AAGCAGTTGGTGGCGAAGAACATCGTCGACATTGATGAAGTAAATAAGAAAATTGAGATGGAGGTGATGTCGTTTGGGTC
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

80.87

99.424

0.804
  pilT Vibrio cholerae strain A1552

80.87

99.424

0.804
  pilT Acinetobacter baylyi ADP1

69.184

95.389

0.66
  pilT Pseudomonas aeruginosa PAK

67.059

97.983

0.657
  pilT Acinetobacter baumannii D1279779

68.693

94.813

0.651
  pilT Acinetobacter baumannii strain A118

68.693

94.813

0.651
  pilT Pseudomonas stutzeri DSM 10701

66.766

97.118

0.648
  pilT Acinetobacter nosocomialis M2

68.389

94.813

0.648
  pilT Legionella pneumophila strain Lp02

67.477

94.813

0.64
  pilT Legionella pneumophila strain ERS1305867

67.477

94.813

0.64
  pilT Neisseria meningitidis 8013

60.519

100

0.605
  pilT Neisseria gonorrhoeae MS11

60.231

100

0.602
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.102

96.254

0.473
  pilU Vibrio cholerae strain A1552

43.077

93.66

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.673

94.236

0.383
  pilU Acinetobacter baylyi ADP1

41.121

92.507

0.38