Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L3V77_RS02085 Genome accession   NZ_CP091975
Coordinates   418205..419326 (+) Length   373 a.a.
NCBI ID   WP_275135506.1    Uniprot ID   -
Organism   Vibrio sp. DW001     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413205..424326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3V77_RS02055 (L3V77_02055) - 414052..414651 (-) 600 WP_275135500.1 XTP/dITP diphosphatase -
  L3V77_RS02060 (L3V77_02060) yggU 414690..414977 (-) 288 WP_275135501.1 DUF167 family protein YggU -
  L3V77_RS02065 (L3V77_02065) - 414977..415531 (-) 555 WP_275135502.1 YggT family protein -
  L3V77_RS02070 (L3V77_02070) proC 415573..416391 (-) 819 WP_275135503.1 pyrroline-5-carboxylate reductase -
  L3V77_RS02075 (L3V77_02075) - 416422..417132 (-) 711 WP_275135504.1 YggS family pyridoxal phosphate-dependent enzyme -
  L3V77_RS02080 (L3V77_02080) pilT 417157..418200 (+) 1044 WP_275135505.1 type IV pilus twitching motility protein PilT Machinery gene
  L3V77_RS02085 (L3V77_02085) pilU 418205..419326 (+) 1122 WP_275135506.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L3V77_RS02090 (L3V77_02090) ruvX 419376..419807 (-) 432 WP_275135507.1 Holliday junction resolvase RuvX -
  L3V77_RS02095 (L3V77_02095) - 419868..420431 (-) 564 WP_275135508.1 YqgE/AlgH family protein -
  L3V77_RS02100 (L3V77_02100) gshB 420450..421397 (-) 948 WP_275135509.1 glutathione synthase -
  L3V77_RS02105 (L3V77_02105) rsmE 421432..422163 (-) 732 WP_275135510.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L3V77_RS02110 (L3V77_02110) - 422282..422782 (-) 501 WP_275135511.1 SprT family zinc-dependent metalloprotease -
  L3V77_RS02115 (L3V77_02115) - 422841..423635 (-) 795 WP_195702149.1 DUF2189 domain-containing protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41788.85 Da        Isoelectric Point: 6.4356

>NTDB_id=654778 L3V77_RS02085 WP_275135506.1 418205..419326(+) (pilU) [Vibrio sp. DW001]
MDFNKAIDINTLLHEMSVNKASDIYITVDSPCLQRIDGQLRAIGEKLNKERVSFLLNALMDDEKKSEFNRTKEANFAVVR
NSGRYRVSAFFQRELPGAVIRRIETEIPTFDELMLPEIMKELSVAKRGLVLIVGSTGSGKSTTMAAMTGYRNQNRSGHIL
TVEDPIEFVHEHDQCIVTQREVGVDTESYEVALKNSLRQAPDMILIGEIRSRETMEFAMTFAETGHLCMATLHANNANQA
LERILNLVPKNHKEQFLFDLSSNLKGIIGQQLIIDKHGQGRHGVFEVLLNTPRVSDLIRRGDLHELKPTMVKSTESGMRT
FDQSLYQLVVEGKITEVDALHSADSANDLRLMMKTNKGELNSSALAGVKVDMG

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=654778 L3V77_RS02085 WP_275135506.1 418205..419326(+) (pilU) [Vibrio sp. DW001]
ATGGATTTTAACAAGGCTATTGATATCAATACTCTTCTGCATGAGATGAGCGTTAATAAAGCGTCAGATATATATATTAC
GGTCGATTCGCCCTGCCTGCAGCGTATTGATGGGCAGTTGCGTGCCATCGGAGAAAAACTGAATAAAGAGCGCGTTTCTT
TTTTGCTCAATGCTCTTATGGATGATGAAAAAAAATCGGAATTTAATCGAACTAAAGAAGCGAACTTTGCGGTAGTACGC
AACAGTGGCCGATATCGTGTGAGTGCTTTTTTTCAACGAGAGCTGCCTGGTGCTGTGATAAGAAGAATAGAGACAGAAAT
CCCTACCTTTGATGAATTGATGCTCCCAGAGATAATGAAAGAGCTTTCTGTTGCCAAAAGAGGACTCGTTTTGATTGTCG
GTTCTACTGGTTCAGGTAAATCGACTACCATGGCAGCAATGACTGGATATCGAAATCAAAACAGAAGCGGTCATATTTTG
ACAGTGGAAGACCCAATTGAATTTGTTCATGAACATGACCAATGCATTGTTACGCAAAGGGAGGTTGGCGTTGATACTGA
AAGCTATGAAGTTGCATTGAAAAATTCACTTCGCCAAGCACCAGATATGATTTTAATTGGTGAGATTCGTAGTCGTGAAA
CCATGGAATTTGCCATGACATTTGCCGAGACAGGCCATCTATGTATGGCGACATTGCACGCAAACAATGCGAATCAAGCG
TTAGAACGTATTCTTAATCTGGTTCCTAAAAATCATAAAGAACAGTTCTTATTTGACCTATCGAGCAACTTGAAGGGCAT
AATTGGCCAGCAGTTGATTATAGATAAACATGGACAAGGTCGACATGGTGTTTTTGAGGTCTTACTTAATACACCAAGAG
TTTCCGATCTTATTCGGCGCGGTGATCTTCATGAACTGAAACCAACAATGGTTAAATCAACAGAATCAGGGATGAGGACA
TTTGACCAGTCGCTTTACCAACTTGTGGTAGAAGGCAAAATCACAGAAGTTGATGCATTACACAGTGCTGATTCGGCAAA
TGATTTACGACTAATGATGAAGACAAATAAGGGTGAACTCAACAGTAGTGCATTGGCTGGTGTAAAGGTGGATATGGGGT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

74.932

98.391

0.737
  pilU Pseudomonas stutzeri DSM 10701

53.846

100

0.544
  pilU Acinetobacter baylyi ADP1

52.137

94.102

0.491
  pilT Legionella pneumophila strain ERS1305867

40.708

90.885

0.37
  pilT Legionella pneumophila strain Lp02

40.708

90.885

0.37
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.294

91.153

0.367
  pilT Acinetobacter baumannii D1279779

39.823

90.885

0.362
  pilT Acinetobacter baumannii strain A118

39.823

90.885

0.362
  pilT Acinetobacter nosocomialis M2

39.823

90.885

0.362
  pilT Pseudomonas aeruginosa PAK

39.823

90.885

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.785

88.74

0.362
  pilT Vibrio cholerae strain A1552

40.785

88.74

0.362