Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5M76_RS15490 Genome accession   NZ_CP091833
Coordinates   3525607..3526644 (-) Length   345 a.a.
NCBI ID   WP_037420775.1    Uniprot ID   A0A501XE78
Organism   Shewanella xiamenensis strain HD6452     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3520607..3531644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5M76_RS15455 (K5M76_15420) - 3520771..3521085 (+) 315 WP_224021806.1 hypothetical protein -
  K5M76_RS15460 (K5M76_15425) yciH 3521248..3521577 (+) 330 WP_047533731.1 stress response translation initiation inhibitor YciH -
  K5M76_RS15465 (K5M76_15430) - 3521646..3522209 (+) 564 WP_037429424.1 YqgE/AlgH family protein -
  K5M76_RS15470 (K5M76_15435) ruvX 3522239..3522664 (+) 426 WP_037420762.1 Holliday junction resolvase RuvX -
  K5M76_RS15475 (K5M76_15440) hemH 3522728..3523741 (-) 1014 WP_181159868.1 ferrochelatase -
  K5M76_RS15480 (K5M76_15445) - 3523780..3524313 (-) 534 WP_224021805.1 glutathione peroxidase -
  K5M76_RS15485 (K5M76_15450) pilU 3524485..3525597 (-) 1113 WP_037420771.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5M76_RS15490 (K5M76_15455) pilT 3525607..3526644 (-) 1038 WP_037420775.1 type IV pilus twitching motility protein PilT Machinery gene
  K5M76_RS15495 (K5M76_15460) - 3526680..3527393 (+) 714 WP_224021804.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5M76_RS15500 (K5M76_15465) proC 3527617..3528435 (+) 819 WP_047533742.1 pyrroline-5-carboxylate reductase -
  K5M76_RS15505 (K5M76_15470) - 3528510..3529058 (+) 549 WP_037420784.1 YggT family protein -
  K5M76_RS15510 (K5M76_15475) yggU 3529058..3529348 (+) 291 WP_037420787.1 DUF167 family protein YggU -
  K5M76_RS15515 (K5M76_15480) - 3529422..3529856 (+) 435 WP_037420790.1 DUF4426 domain-containing protein -
  K5M76_RS15520 (K5M76_15485) rdgB 3529971..3530588 (+) 618 WP_224021803.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38278.98 Da        Isoelectric Point: 6.9096

>NTDB_id=653809 K5M76_RS15490 WP_037420775.1 3525607..3526644(-) (pilT) [Shewanella xiamenensis strain HD6452]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHPNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=653809 K5M76_RS15490 WP_037420775.1 3525607..3526644(-) (pilT) [Shewanella xiamenensis strain HD6452]
ATGGAAATCACTGAGTTATTAGCTTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATCAATCTGCCCGCGCTCGATCATCAGGGTGTACACAGCCTTGTTTATGACA
TTATGAATGATAAACAACGCAAGGACTTTGAAGAACATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTGAACGCCTTTAACCAATCTCGCGGCGCTGCGGCGGTATTTCGTACCATTCCCAGTGATATCTTGTCACTTGA
GCAGCTAGGTGCGCCTGAAATTTTTAAAAAAATTGCCAGTTTTCCCCGTGGGTTAGTGTTAGTCACTGGGCCTACAGGTT
CCGGTAAGAGTACCACACTTGCGGCCATGGTCGATTACATCAATGAAAACCGCCATGATCATATCTTAACCATTGAAGAC
CCAATCGAATTTGTTCATCCCAACAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGTCATACCCACAGCTTTAACGC
GGCGCTGCGTAGCGCATTGCGTGAAGACCCTGACGTTATCCTCGTGGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CGATGACGGCGGCTGAAACGGGTCACTTAGTTTTTGGCACCTTACACACCACGTCGGCGGCTAAGACTATTGACCGTGTG
GTTGACGTTTTCCCTGCCGGTGAAAAGGACATGGTGCGTACCATGTTGTCTGAATCATTACAGGCGGTTATTTCGCAAAC
CCTGATTAAGAAAATCGGTGGTGGTCGTGTTGCTGCCCACGAAATTATGATGGGTACGCCCGCTATCCGAAATCTTATCC
GTGAAGATAAAGTGGCGCAGATGTACTCAGCCATTCAAACGGGGATGGCCCATGGCATGCAAACACTCGAACAGTGTCTG
CAAAACTTAGTGAACCGTGGCCTCATCACCCGTGAGGATGCCATGGCGAAGAGCTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A501XE78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Acinetobacter nosocomialis M2

80.117

99.13

0.794
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.691

99.42

0.414
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391