Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5M76_RS15485 Genome accession   NZ_CP091833
Coordinates   3524485..3525597 (-) Length   370 a.a.
NCBI ID   WP_037420771.1    Uniprot ID   -
Organism   Shewanella xiamenensis strain HD6452     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3519485..3530597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5M76_RS15450 (K5M76_15415) - 3520032..3520592 (-) 561 WP_037420751.1 DUF4136 domain-containing protein -
  K5M76_RS15455 (K5M76_15420) - 3520771..3521085 (+) 315 WP_224021806.1 hypothetical protein -
  K5M76_RS15460 (K5M76_15425) yciH 3521248..3521577 (+) 330 WP_047533731.1 stress response translation initiation inhibitor YciH -
  K5M76_RS15465 (K5M76_15430) - 3521646..3522209 (+) 564 WP_037429424.1 YqgE/AlgH family protein -
  K5M76_RS15470 (K5M76_15435) ruvX 3522239..3522664 (+) 426 WP_037420762.1 Holliday junction resolvase RuvX -
  K5M76_RS15475 (K5M76_15440) hemH 3522728..3523741 (-) 1014 WP_181159868.1 ferrochelatase -
  K5M76_RS15480 (K5M76_15445) - 3523780..3524313 (-) 534 WP_224021805.1 glutathione peroxidase -
  K5M76_RS15485 (K5M76_15450) pilU 3524485..3525597 (-) 1113 WP_037420771.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5M76_RS15490 (K5M76_15455) pilT 3525607..3526644 (-) 1038 WP_037420775.1 type IV pilus twitching motility protein PilT Machinery gene
  K5M76_RS15495 (K5M76_15460) - 3526680..3527393 (+) 714 WP_224021804.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5M76_RS15500 (K5M76_15465) proC 3527617..3528435 (+) 819 WP_047533742.1 pyrroline-5-carboxylate reductase -
  K5M76_RS15505 (K5M76_15470) - 3528510..3529058 (+) 549 WP_037420784.1 YggT family protein -
  K5M76_RS15510 (K5M76_15475) yggU 3529058..3529348 (+) 291 WP_037420787.1 DUF167 family protein YggU -
  K5M76_RS15515 (K5M76_15480) - 3529422..3529856 (+) 435 WP_037420790.1 DUF4426 domain-containing protein -
  K5M76_RS15520 (K5M76_15485) rdgB 3529971..3530588 (+) 618 WP_224021803.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41117.37 Da        Isoelectric Point: 6.5054

>NTDB_id=653808 K5M76_RS15485 WP_037420771.1 3524485..3525597(-) (pilU) [Shewanella xiamenensis strain HD6452]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESVMTEAQKKEFHTTRECNFAFAVKELGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVAKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKEPASSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=653808 K5M76_RS15485 WP_037420771.1 3524485..3525597(-) (pilU) [Shewanella xiamenensis strain HD6452]
ATGGATGTCCGTCCGTTTTTAAAAGTCATGGTGGAGCGTAAAGCCTCGGACTTGTTTATTACCGCAGGTTTTCCGCCTAG
CGCCAAAATTGATGGCGAGTTACGCCCATTAGCCGAGAGCGCCTTTACGCCCGCGCAGTCTTTGGATTTTGTCGAGTCCG
TGATGACGGAGGCGCAAAAGAAGGAATTTCACACGACTCGCGAGTGTAACTTTGCTTTTGCGGTTAAGGAGTTGGGTCGT
TTTCGCGTCAGCGCATTCTGGCAGCGTGAGTCTCCGGGTTGTGTTATGCGCCGGATTGAGACCAAAATCCCTGAAGTGGA
TGATTTAAAACTGCCACCGATTTTAAAAGATCTGGTGATGAGTAAGCGTGGACTTATCATCATGGTGGGGGGGACAGGGA
CCGGTAAATCGACTTCCTTAGCTGCATTAGTCGGTTACCGCAATGCCCATGCCCGCGGGCATATCTTGACGATTGAAGAC
CCTGTGGAATTTGTTCACGATCATCGCAAAAGTATCATTACTCAACGTGAAGTGGGCATAGATACAGAGTCCTTTGATGC
GGCGCTGAAAAGCTCGTTGCGTCAGGCGCCTGATGTGATCCTTATCGGTGAAATTCGAACCCAAGAAACCATGGAGTTTG
CGCTCTCCTTTGCTGAAACGGGCCATCTGTGTATGGCGACACTGCATGCTAACAATGCTAACCAAGCATTAGACCGGATT
ATGCACTTAGTGCCAGAGAGTAAACATCAACAGCTATTATTCGATTTGTCACTCAACCTGCGCGGTATTGTGGCGCAGCA
GCTGGTGGCTAAAGTTGATGGCACAGGGCGCCGGGCGGCGATTGAAGTCTTGATCAATACGCCACGTGTCGCGAGTTTGA
TTGCTAAAAACGAGCTGCATTTGCTCAAAGAAACCATGGCCAAATCGAATGAGCAAGGAATGCAGACTTTTGACCAAGCT
CTGTTAAAGCTCTATGTCGATGGGGAGATCAGTTATGCCGACGCGCTTCACCATGCTGACTCACCCAACGACTTGCGGTT
AATGATTAAATTGCAAAATAAAGAACCCGCCAGTTCGAGCTTTATGGAAGGCGTGACCTTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.108

100

0.603
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.988

88.649

0.381
  pilT Vibrio cholerae strain A1552

42.988

88.649

0.381
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.29

93.243

0.376
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Legionella pneumophila strain Lp02

40.785

89.459

0.365
  pilT Legionella pneumophila strain ERS1305867

40.785

89.459

0.365