Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SUTH_RS01320 Genome accession   NZ_AP012547
Coordinates   270778..271821 (+) Length   347 a.a.
NCBI ID   WP_041096478.1    Uniprot ID   W0SEA9
Organism   Sulfuritalea hydrogenivorans sk43H strain DSM 22779     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 265778..276821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SUTH_RS01295 (SUTH_00262) - 266913..268049 (-) 1137 WP_052473039.1 DEAD/DEAH box helicase -
  SUTH_RS01300 (SUTH_00263) - 268065..268358 (-) 294 WP_041096470.1 DUF167 family protein -
  SUTH_RS01305 (SUTH_00264) - 268462..269040 (-) 579 WP_041096472.1 YggT family protein -
  SUTH_RS01310 (SUTH_00265) proC 269040..269852 (-) 813 WP_041096474.1 pyrroline-5-carboxylate reductase -
  SUTH_RS01315 (SUTH_00266) - 270045..270731 (-) 687 WP_041096476.1 YggS family pyridoxal phosphate-dependent enzyme -
  SUTH_RS01320 (SUTH_00267) pilT 270778..271821 (+) 1044 WP_041096478.1 type IV pilus twitching motility protein PilT Machinery gene
  SUTH_RS01325 (SUTH_00268) pilU 271943..273079 (+) 1137 WP_041096479.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SUTH_RS20300 - 273087..273533 (-) 447 WP_408054971.1 CZB domain-containing protein -
  SUTH_RS20160 (SUTH_00269) - 273558..274565 (-) 1008 Protein_267 methyl-accepting chemotaxis protein -
  SUTH_RS01335 (SUTH_00270) - 274540..275058 (-) 519 WP_197539631.1 PAS domain-containing protein -
  SUTH_RS01340 (SUTH_00271) - 275055..276230 (-) 1176 WP_041096481.1 class I SAM-dependent RNA methyltransferase -
  SUTH_RS01345 (SUTH_00272) - 276233..276649 (-) 417 WP_041096483.1 CopD family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38377.21 Da        Isoelectric Point: 7.0671

>NTDB_id=65347 SUTH_RS01320 WP_041096478.1 270778..271821(+) (pilT) [Sulfuritalea hydrogenivorans sk43H strain DSM 22779]
MDITELLTFVVKNKASDLHLSSGMPPMIRVHGDVRRINLPAMEHKDVHAMIYDIMNDGQRKSYEENLECDFSFAVPNLAR
FRVNAFVQNRGAAAVMRTIPSKILSLEELKAPKIFEELADYPRGLVLVTGPTGSGKSTTLAAMVNHKNESEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLAMSGAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKEMIRAMLSESLRAVISQTLCKTMDGTGRVAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGQQFGMQTLDQN
LQDLVKRKVIAPSEARAKAANKDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=65347 SUTH_RS01320 WP_041096478.1 270778..271821(+) (pilT) [Sulfuritalea hydrogenivorans sk43H strain DSM 22779]
ATGGACATCACCGAACTGCTCACCTTCGTCGTCAAGAACAAGGCGTCCGATTTGCATCTGTCCTCAGGCATGCCGCCGAT
GATCCGCGTCCATGGCGACGTACGGCGCATCAACCTGCCGGCCATGGAGCACAAGGATGTGCATGCCATGATCTATGACA
TCATGAACGACGGGCAACGAAAGTCCTACGAGGAAAATCTCGAGTGCGACTTTTCGTTCGCGGTGCCGAACCTGGCGCGC
TTCCGCGTCAATGCCTTTGTCCAGAACCGCGGCGCGGCGGCGGTGATGCGGACCATTCCGTCGAAAATCCTTTCGCTGGA
GGAACTCAAGGCGCCGAAAATCTTTGAAGAACTGGCCGACTATCCGCGCGGCCTGGTGCTGGTGACGGGGCCGACCGGTT
CGGGAAAGTCGACCACGCTGGCCGCGATGGTCAATCACAAGAACGAGAGCGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGTCCGCATACCCTGTCCTTCAACAA
CGCCCTGCGCTCGGCCTTGCGCGAAGACCCGGACGTGATCCTGGTCGGCGAAATGCGCGACCTGGAAACCATCCGCCTCG
CCATGTCCGGCGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACTTCGTCGGCGGCGAAAACCATCGACCGGATC
ATCGACGTCTTCCCCGCCGCGGAAAAGGAAATGATCCGCGCCATGCTGTCGGAATCGCTGCGGGCGGTGATCTCGCAGAC
CCTGTGCAAGACCATGGACGGCACCGGCCGCGTTGCTGCGCACGAGATCATGGTCGGCACGCCGGCCATCCGCAACCTGA
TTCGCGAGGCCAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCCAGCAGTTCGGCATGCAGACGCTGGACCAGAAC
CTGCAGGACCTGGTGAAGAGAAAGGTCATCGCTCCGAGCGAGGCGCGCGCCAAGGCCGCCAACAAAGACAACTTCCCCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0SEA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Pseudomonas aeruginosa PAK

73.333

99.424

0.729
  pilT Pseudomonas stutzeri DSM 10701

73.043

99.424

0.726
  pilT Legionella pneumophila strain Lp02

72.174

99.424

0.718
  pilT Legionella pneumophila strain ERS1305867

72.174

99.424

0.718
  pilT Neisseria meningitidis 8013

70.145

99.424

0.697
  pilT Neisseria gonorrhoeae MS11

69.855

99.424

0.695
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.343

97.406

0.666
  pilT Vibrio cholerae strain A1552

68.343

97.406

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.424

0.496
  pilU Vibrio cholerae strain A1552

41.983

98.847

0.415
  pilU Acinetobacter baylyi ADP1

41.379

100

0.415
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.424

0.401

Multiple sequence alignment