Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SUTH_RS01325 Genome accession   NZ_AP012547
Coordinates   271943..273079 (+) Length   378 a.a.
NCBI ID   WP_041096479.1    Uniprot ID   -
Organism   Sulfuritalea hydrogenivorans sk43H strain DSM 22779     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 266943..278079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SUTH_RS01300 (SUTH_00263) - 268065..268358 (-) 294 WP_041096470.1 DUF167 family protein -
  SUTH_RS01305 (SUTH_00264) - 268462..269040 (-) 579 WP_041096472.1 YggT family protein -
  SUTH_RS01310 (SUTH_00265) proC 269040..269852 (-) 813 WP_041096474.1 pyrroline-5-carboxylate reductase -
  SUTH_RS01315 (SUTH_00266) - 270045..270731 (-) 687 WP_041096476.1 YggS family pyridoxal phosphate-dependent enzyme -
  SUTH_RS01320 (SUTH_00267) pilT 270778..271821 (+) 1044 WP_041096478.1 type IV pilus twitching motility protein PilT Machinery gene
  SUTH_RS01325 (SUTH_00268) pilU 271943..273079 (+) 1137 WP_041096479.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SUTH_RS20300 - 273087..273533 (-) 447 WP_408054971.1 CZB domain-containing protein -
  SUTH_RS20160 (SUTH_00269) - 273558..274565 (-) 1008 Protein_267 methyl-accepting chemotaxis protein -
  SUTH_RS01335 (SUTH_00270) - 274540..275058 (-) 519 WP_197539631.1 PAS domain-containing protein -
  SUTH_RS01340 (SUTH_00271) - 275055..276230 (-) 1176 WP_041096481.1 class I SAM-dependent RNA methyltransferase -
  SUTH_RS01345 (SUTH_00272) - 276233..276649 (-) 417 WP_041096483.1 CopD family protein -
  SUTH_RS01350 (SUTH_00273) - 276637..277527 (-) 891 WP_041096485.1 NAD-dependent epimerase/dehydratase family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42456.17 Da        Isoelectric Point: 6.5085

>NTDB_id=65348 SUTH_RS01325 WP_041096479.1 271943..273079(+) (pilU) [Sulfuritalea hydrogenivorans sk43H strain DSM 22779]
MERDEGLKFMYQLLTMMMQKKGSDLFITSGFPPAMKIDGKMTPATTQPLSSQHTQELARAIMNDKQAAEFEATKECNFAI
SPASIGRFRVNAFVQQGRVGMILRTINVAIPEMEDLKLPPVLKDVVMTKRGLVLFVGGTGSGKSTSLAAMIGYRNQNSYG
HIITIEDPVEYVHDHRNCVITQREVGVDTDSWEIALKNTLRQAPDVILIGEIRDREVMEHAIAFAETGHLAMGTLHANNA
NQAIDRIINFFPEERRPQLLMDLSLNLKAIVSQRLIPVKEGKGRAAAVEILLNSPLIADLIFKGEIHEVKEIMKKSRELG
MKTFDMALFDLFEEGRISYDDALRFADSMNEVRLMIKLHSKQSQEMDRNSGIQHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=65348 SUTH_RS01325 WP_041096479.1 271943..273079(+) (pilU) [Sulfuritalea hydrogenivorans sk43H strain DSM 22779]
ATGGAACGCGATGAAGGCCTGAAGTTCATGTACCAGCTTCTGACGATGATGATGCAGAAGAAGGGCTCCGACTTGTTCAT
TACCTCCGGCTTCCCGCCGGCGATGAAGATCGACGGCAAGATGACGCCGGCGACCACCCAGCCCTTGTCGTCGCAGCACA
CGCAGGAGCTGGCGCGCGCGATCATGAACGACAAGCAGGCCGCCGAGTTCGAGGCGACCAAGGAATGCAACTTCGCGATC
TCCCCCGCCAGTATCGGCCGCTTCCGCGTCAATGCCTTCGTCCAGCAGGGCCGCGTCGGCATGATCCTGCGCACCATCAA
CGTGGCGATTCCGGAAATGGAAGACCTCAAGCTGCCGCCGGTACTCAAGGACGTGGTGATGACCAAGCGCGGCCTGGTGT
TGTTCGTCGGCGGCACGGGTTCCGGCAAGTCGACCTCGTTGGCGGCCATGATCGGCTATCGCAACCAGAACAGTTACGGC
CACATCATCACCATCGAAGACCCGGTCGAATATGTTCACGACCATCGCAATTGCGTGATTACCCAGCGCGAGGTCGGCGT
CGATACCGACTCATGGGAAATCGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTCATCCTGATCGGTGAAATCCGCG
ACCGGGAAGTGATGGAGCACGCCATCGCCTTTGCCGAAACCGGCCATCTGGCGATGGGCACGCTGCACGCCAACAACGCC
AACCAGGCGATCGATCGCATCATCAACTTCTTCCCGGAAGAGCGTCGGCCGCAGCTGTTGATGGATCTCTCGCTCAACCT
CAAGGCCATCGTCTCGCAGCGCCTGATCCCTGTGAAGGAAGGCAAGGGCCGCGCGGCGGCGGTGGAGATATTGCTCAATT
CCCCGCTGATTGCCGACCTGATCTTCAAGGGCGAAATTCACGAGGTCAAGGAGATCATGAAGAAGTCCAGGGAACTCGGC
ATGAAGACCTTCGACATGGCGCTCTTCGACCTCTTCGAGGAAGGTCGCATCAGTTACGACGATGCCCTGCGTTTTGCCGA
TTCGATGAACGAAGTGCGGCTCATGATCAAGCTGCACAGCAAGCAGTCGCAGGAGATGGATCGCAACAGCGGCATCCAGC
ACCTCGACATCGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.124

93.651

0.601
  pilU Acinetobacter baylyi ADP1

58.146

94.18

0.548
  pilU Vibrio cholerae strain A1552

56.358

91.534

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.114

88.36

0.381
  pilT Legionella pneumophila strain Lp02

42.598

87.566

0.373
  pilT Legionella pneumophila strain ERS1305867

42.598

87.566

0.373
  pilT Pseudomonas aeruginosa PAK

42.296

87.566

0.37
  pilT Acinetobacter baylyi ADP1

40.95

89.153

0.365
  pilT Pseudomonas stutzeri DSM 10701

41.692

87.566

0.365
  pilT Acinetobacter nosocomialis M2

41.896

86.508

0.362
  pilT Acinetobacter baumannii D1279779

41.896

86.508

0.362
  pilT Acinetobacter baumannii strain A118

41.896

86.508

0.362

Multiple sequence alignment