Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LZ558_RS19490 Genome accession   NZ_CP091424
Coordinates   4397224..4398261 (+) Length   345 a.a.
NCBI ID   WP_268118556.1    Uniprot ID   -
Organism   Methylobacter sp. YRD-M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4392224..4403261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ558_RS19465 (LZ558_19415) - 4393118..4394461 (-) 1344 WP_326498424.1 selenium-binding protein SBP56-related protein -
  LZ558_RS19470 (LZ558_19420) - 4394470..4394742 (-) 273 WP_268118552.1 DUF4242 domain-containing protein -
  LZ558_RS19475 (LZ558_19425) - 4394801..4395397 (-) 597 WP_268118553.1 TetR/AcrR family transcriptional regulator -
  LZ558_RS19480 (LZ558_19430) - 4395755..4396417 (+) 663 WP_268118554.1 hypothetical protein -
  LZ558_RS19485 (LZ558_19435) - 4396429..4397112 (-) 684 WP_268118555.1 YggS family pyridoxal phosphate-dependent enzyme -
  LZ558_RS19490 (LZ558_19440) pilT 4397224..4398261 (+) 1038 WP_268118556.1 type IV pilus twitching motility protein PilT Machinery gene
  LZ558_RS19495 (LZ558_19445) pilU 4398271..4399404 (+) 1134 WP_268118557.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LZ558_RS19500 (LZ558_19450) djlA 4399456..4400262 (-) 807 WP_268118558.1 co-chaperone DjlA -
  LZ558_RS19505 (LZ558_19455) - 4400336..4401229 (+) 894 WP_268118559.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  LZ558_RS19510 (LZ558_19460) radC 4401233..4401907 (-) 675 WP_268118560.1 DNA repair protein RadC -
  LZ558_RS19515 (LZ558_19465) coaBC 4402002..4403198 (+) 1197 WP_268120866.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38521.43 Da        Isoelectric Point: 6.8938

>NTDB_id=651100 LZ558_RS19490 WP_268118556.1 4397224..4398261(+) (pilT) [Methylobacter sp. YRD-M1]
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDIRRINIPALEHKEVHALMYDIMNDKQRRDYEEFLETDFSFELPGIAR
FRVNAFNQERGAAGVFRTIPSKVMSLEELKAPRFFEELTRKPRGLILVTGPTGSGKSTTLAAMINHININDYAHILTVED
PIEFVHESQKCLINQREVHRDTHGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLLKKIGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGLITAKTAMVKAVNKDIFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=651100 LZ558_RS19490 WP_268118556.1 4397224..4398261(+) (pilT) [Methylobacter sp. YRD-M1]
ATGGATATTGCCGAGTTATTGGCCTTTTCAGTTAAAAACAACGCGTCGGATTTGCATCTGTCGGCCGGCCTGCCGCCCAT
GATCAGGGTCGATGGCGATATCCGCCGCATCAATATTCCCGCTCTGGAGCATAAGGAAGTGCATGCGCTGATGTATGACA
TCATGAACGACAAGCAGCGCCGCGATTATGAAGAATTTCTGGAAACCGACTTTTCTTTCGAATTGCCCGGCATTGCCAGG
TTCCGTGTCAACGCATTCAATCAGGAGCGCGGTGCGGCCGGCGTTTTCAGAACGATTCCGTCCAAAGTCATGAGCCTTGA
AGAGTTGAAGGCGCCCAGGTTTTTTGAGGAACTGACGCGCAAACCGCGCGGCCTGATTCTGGTGACGGGCCCTACCGGCT
CGGGCAAATCGACAACGCTGGCGGCCATGATCAACCATATCAACATCAACGATTACGCGCATATCCTGACCGTTGAAGAC
CCGATCGAATTTGTGCACGAAAGCCAGAAATGCCTGATCAACCAGCGCGAGGTTCACCGCGACACCCACGGCTTCAATGA
AGCGTTGCGCTCGGCGCTGCGTGAAGACCCGGACGTTATTCTGGTCGGTGAGATGCGCGACCTGGAAACGATCAGGCTGG
CCCTGACCGCAGCCGAAACCGGCCACCTGGTATTCGGAACCTTGCACACCTCCTCGGCCGCCAAAACCATCGACCGTATT
ATCGACGTCTTCCCGGCCGCCGAAAAAGACATGATCCGCTCGATGCTGTCCGAATCGCTGCAGGCGGTTATTTCGCAAAC
CCTGCTCAAGAAAATCGGCGGCGGCCGTATTGCCGCGCATGAAATCATGGTCGGCACGCCCGCGATCAGAAACCTGATCC
GCGAAGCCAAGGTCGCGCAAATGTACTCAGCCATCCAGACGGGCCGGAAAGACGGCATGCAGACGCTTGATCAGAACCTG
AAAGAATTGGTCGACAAAGGCCTGATTACCGCCAAGACCGCGATGGTCAAAGCCGTCAATAAAGATATATTCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Legionella pneumophila strain Lp02

73.256

99.71

0.73
  pilT Legionella pneumophila strain ERS1305867

73.256

99.71

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.681

0.684
  pilT Vibrio cholerae strain A1552

70.03

97.681

0.684
  pilT Neisseria meningitidis 8013

67.055

99.42

0.667
  pilT Neisseria gonorrhoeae MS11

66.764

99.42

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Pseudomonas stutzeri DSM 10701

40.462

100

0.406
  pilU Acinetobacter baylyi ADP1

40.882

98.551

0.403
  pilU Vibrio cholerae strain A1552

39.306

100

0.394