Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LZ558_RS19495 Genome accession   NZ_CP091424
Coordinates   4398271..4399404 (+) Length   377 a.a.
NCBI ID   WP_268118557.1    Uniprot ID   -
Organism   Methylobacter sp. YRD-M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4393271..4404404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ558_RS19470 (LZ558_19420) - 4394470..4394742 (-) 273 WP_268118552.1 DUF4242 domain-containing protein -
  LZ558_RS19475 (LZ558_19425) - 4394801..4395397 (-) 597 WP_268118553.1 TetR/AcrR family transcriptional regulator -
  LZ558_RS19480 (LZ558_19430) - 4395755..4396417 (+) 663 WP_268118554.1 hypothetical protein -
  LZ558_RS19485 (LZ558_19435) - 4396429..4397112 (-) 684 WP_268118555.1 YggS family pyridoxal phosphate-dependent enzyme -
  LZ558_RS19490 (LZ558_19440) pilT 4397224..4398261 (+) 1038 WP_268118556.1 type IV pilus twitching motility protein PilT Machinery gene
  LZ558_RS19495 (LZ558_19445) pilU 4398271..4399404 (+) 1134 WP_268118557.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LZ558_RS19500 (LZ558_19450) djlA 4399456..4400262 (-) 807 WP_268118558.1 co-chaperone DjlA -
  LZ558_RS19505 (LZ558_19455) - 4400336..4401229 (+) 894 WP_268118559.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  LZ558_RS19510 (LZ558_19460) radC 4401233..4401907 (-) 675 WP_268118560.1 DNA repair protein RadC -
  LZ558_RS19515 (LZ558_19465) coaBC 4402002..4403198 (+) 1197 WP_268120866.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  LZ558_RS19520 (LZ558_19470) dut 4403179..4403634 (+) 456 WP_326498425.1 dUTP diphosphatase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42219.30 Da        Isoelectric Point: 5.3195

>NTDB_id=651101 LZ558_RS19495 WP_268118557.1 4398271..4399404(+) (pilU) [Methylobacter sp. YRD-M1]
MDFKTLLALMVQKKASDLFITAGRAPTMKVDGSLVEVSKSPLTEDQALKIVLSIMNQKQRDEFENTKECQFAISVPSLAR
FRVSAFTQRDAAGMVLRRIENDIPDFESLHLPPVLKDLIMEKRGLVIFVGATGTGKSTSLASLIKYRNQNSSGHIITIED
PMEYEHQHLGCIITQREVGVDTESYEVALKNTLRQAPDVILIGEIRTRETMQNAITFAETGHLCLSTLHANNANQALDRI
LHFFPEEMHDQLFMDLSLNLRGVVAQQLIKRADGKGRYPAVEVLINTPLAKDYIRKGEVNKLKELMKDSREQGMQTFDQA
LYDLYVAGKISYDDALNSADSRNEVRLMIKLGADSSGTFDDNSMVLTETDDDKGVIF

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=651101 LZ558_RS19495 WP_268118557.1 4398271..4399404(+) (pilU) [Methylobacter sp. YRD-M1]
ATGGATTTTAAAACGCTACTTGCATTGATGGTGCAGAAAAAAGCATCCGATTTGTTTATTACGGCAGGCCGGGCCCCGAC
CATGAAAGTCGACGGCTCGCTTGTGGAAGTGTCCAAATCGCCGTTGACGGAAGATCAGGCATTAAAGATCGTCCTCAGCA
TCATGAACCAGAAGCAGAGGGACGAGTTTGAAAACACCAAGGAATGCCAGTTCGCGATCAGCGTGCCGAGCCTGGCTCGA
TTCAGGGTCAGCGCCTTCACGCAGCGCGATGCGGCCGGCATGGTGCTCCGGCGTATTGAAAATGATATTCCGGATTTCGA
AAGCCTGCATTTGCCGCCCGTATTGAAAGACCTGATCATGGAAAAACGCGGCCTGGTCATTTTCGTCGGCGCGACCGGCA
CCGGTAAATCCACCTCGCTGGCGTCATTGATCAAATACCGCAACCAGAACAGCAGCGGCCACATCATCACGATCGAAGAC
CCGATGGAGTACGAACATCAGCACCTGGGCTGCATCATCACCCAGCGCGAAGTCGGCGTCGATACCGAATCCTATGAAGT
CGCGTTAAAAAATACCCTGCGGCAGGCGCCCGATGTCATCCTGATCGGCGAGATCAGAACGCGCGAAACCATGCAGAATG
CGATCACCTTCGCCGAAACCGGGCATTTATGCCTGTCGACGCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGGATT
CTGCATTTCTTTCCCGAAGAGATGCACGATCAATTGTTCATGGACTTGTCGCTGAACCTGCGCGGCGTCGTAGCCCAGCA
GCTGATCAAGCGCGCGGACGGCAAAGGCCGCTATCCGGCCGTTGAAGTTTTGATCAATACGCCGCTGGCCAAGGATTACA
TCCGCAAAGGCGAGGTCAACAAGCTCAAGGAGCTCATGAAAGACTCGCGCGAGCAAGGCATGCAGACGTTTGACCAGGCG
CTGTACGACCTGTATGTCGCCGGCAAGATCAGCTACGACGACGCCCTCAACTCGGCCGACTCCAGAAATGAAGTACGCCT
GATGATCAAGCTGGGCGCTGACAGCTCCGGCACTTTTGACGATAACAGCATGGTATTGACGGAAACCGATGACGACAAGG
GCGTGATTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.211

100

0.647
  pilU Acinetobacter baylyi ADP1

63.536

96.021

0.61
  pilU Vibrio cholerae strain A1552

57.143

92.838

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.934

96.552

0.395
  pilT Legionella pneumophila strain ERS1305867

41.691

90.981

0.379
  pilT Legionella pneumophila strain Lp02

41.691

90.981

0.379
  pilT Acinetobacter nosocomialis M2

41.003

89.92

0.369
  pilT Acinetobacter baumannii strain A118

41.003

89.92

0.369
  pilT Acinetobacter baumannii D1279779

41.003

89.92

0.369
  pilT Pseudomonas aeruginosa PAK

41.003

89.92

0.369
  pilT Acinetobacter baylyi ADP1

40.469

90.451

0.366